Republic of the Philippines

Department of Agriculture
Bureau of Fisheries and Aquatic Resources
National Integrated Fisheries Technology Development Center
MICROBIOLOGY AND FISH HEALTH LABORATORY
Bonuan Binloc, Dagupan City, Pangasinan, Philippines
Tel. Nos. (075) 649-6086; (075) 649-6068
Email address: bfarniftdc@yahoo.com/ bfarniftdc.microlab@gmail.com

CLINICAL LABORATORY RECORD

CASE CODE NO: AAHL-01-28-2021-001 Date Examined: January 28, 2021

Name of Client : Bureau of Fisheries and Aquatic Resources – Regional Office 1

Address : San Fernando City, La Union, 2500
Specimen : 1 pooled sample of marketable size milkfish ( Chanos chanos) and 2
brackishwater
Average Weight : ~100 grams of marketable size milkfish
Source : Fish Pen at San Fabian, Pangasinan
Analysis Conducted: External and Internal Examination of Fish (Necropsy) | Bacterial Enumeration |
Identification of Random Bacterial Colonies Isolated from Internal Organs
Transported : Live: ____Preserved: ____ Dead (Iced): X_

Laboratory Findings:

Necropsy:
- Fish samples were noted with sulphidy
(rotten-egg and extremely fish odor)
- Eyes were clear with no signs of
inflammations or bruises.
- Internally, pale to grayish white colored
gills with signs of infarcted gill tissues,
liver appeared to be brownish to
yellowish in color.
- Flesh/muscle tissues and other internal
organs were soft.
Examined milkfish Chanos chanos exhibited
brownish to yellowish colored liver and
marbled appearance of the gills with pale and
Bacteriological: necrotizing areas.
Water samples and pieces of tissue of liver, spleen and
kidney were taken for bacteriological testing. Results are as follows:

Table 1.1 – Results of Aerobic Plate Count, Presumptive Vibrio Count and Luminous Bacterial
Count of the internal organs processed
Presumptive Percentage of
Aerobic Plate Count Percentage of Green Luminous
Tissue Processed Vibrio Count Yellow Vibrio Remarks
(cfu/ml) Vibrio Colonies (%) Bacterial (cfu/ml)
(cfu/ml) Colonies (%)
*above the normal range
Kidney 4.1 x 107 TNTC 0% 100% ND for APC and TVC
*above the normal
Liver 1.5 x 105 2.8 X 103 0% 100% ND
range for TVC
*above the normal
Spleen 6.7 x 105 TNTC 0% 100% ND
range for TVC
 Table 1.2 – Result of Aerobic Plate Count, Presumptive Vibrio Count and Luminous Bacterial
Counts of water samples processed
Presumptive Percentage of
Aerobic Plate Count Percentage of Green Luminous
Sample Code Vibrio Count Yellow Vibrio Remarks
(cfu/ml) Vibrio Colonies (%) Bacterial (cfu/ml)
(cfu/ml) Colonies (%)
S1FP 3.2 x 103 1 x 102 0% 100% ND *within the normal range
S2M 1 x 102 0 0% 100% ND *within the normal range

*Normal Range: (Safe/Optimum Levels as per SEAFDEC Manual)

Total Bacterial Count <5.0 x 106 - NORMAL
5.1 x 106-8.9 x 106 - Slightly high
9.0 x 106-5.0 x 107 - High
5.1 x 107-9.0 x 107 - Very high
9.1 x 107-1.5 x 107 - Extremely high
Note: ND – means Not Detected
TNTC – means Too Numerous to Count

Table 1.3 – Bacterial isolates identified from pieces of tissue of liver, spleen and kidney
processed
Cedecea lapagei
Acinetobacter spp.
Predominant Bacterial Isolates
Flavimonas oryzihabitans
Chryseomonas luteola

Remarks:

The bacterial isolates identified were part of the normal microflora in the fish gut and water
which are ubiquitous in nature.

Examined by: Checked by: Noted by:

JOHN CARLO G. GOSILATAR EDITHA C. ROXAS CORDELIA B. NIPALES

Laboratory Analyst Senior Aquaculturist Supervising Aquaculturist
Head, Microbiology and Officer-In-Charge, BFAR - NIFTDC
RIZALYN J. BAUTISTA Molecular Pathology Laboratory
Laboratory Analyst Head, Microbiology and Aquatic
Animal Health Laboratory

NOTE: Result(s) as per sample(s) received and analyzed. This report may be reproduced except in full and with written consent from
BFAR-NIFTDC Microbiology and Fish Health Laboratory Section.