LABORATORY ACTIVITY

BENEDICT’S TEST FOR SUGAR

BY:

CYRSTEL JOY ABUAN

HEIDI PAULINE ABUAN
THEALYSSA SONIA CASTRO
CHARLES SPENCER DELA CRUZ
JUSTINE LEIGH DELA CRUZ
SHIELA MAE LORETO
CLEMCY PEARL MONIS
MARY ANGEL NATURA
KATHLEEN MAE PATACSIL
ALEXANDRA YUZON

The above names are students of BSN – 1A BIOCHEM LAB – 1. We are writing this letter to
you, Mr. Jeff Hexton Otero as a correspondence to the activity and or experiment we performed
and discussed on October 16, 2021 about BENEDICT’S SOLUTION test for sugar. Attached in
here are the results/conclusions as well as the documentation of our performed activity.

MATERIALS:

 TEST TUBE
 ALCOHOL LAMP
 URINE, FRUIT JUICE, MILK, AND MILO
 BENEDICT’S SOLUTION

PROCEDURE:

Before anything else, we made sure that the materials needed are sanitized and was ready to
use. After a whole lot of preparation and delayed performance of this activity due to
circumstances we finally made it happen, thanks to Sir Jeff for the kind consideration. First, we
added 5 ml of Benedict’s solutioninto each of the test tube. Then using a dropper or a pipette we
added 8 dropsHEATING
of each sampleUP THE
(urine, fruit SAMPLES
juice, milk, andIN THE
milo) TEST
into each test tube of 5 ml
Benedict’s carefully and accordingly. After TUBE
which we then observed each of the changes if there
is before heating the solution for a minute using the alcohol lamp.
 DATA AND RESULT

SAMPLES COLOR INTERPRETATION

URINE Blue, no change There were no changes
after a minute. after cooling down for 3
minutes but there are
floating particles in the
solution of the test tube.
FRUIT JUICE Blue/dark brown to There were orange
light green to dark particles in the bottom of
green. the solution of the test
tube.
MILK Blue white to brown After cooling down for 3
to light orange. minutes, there were solid
particles under the solution
of the test tube.
MILO Blue to brown to There were dark particles
yellow to dark in the bottom of the
orange. solution of the test tube.

Benedict’s solution is use to test for simple carbohydrates like glucose. Benedict’s
solution is blue, if simple carbohydrates are present, it will change color – green/yellow
if the amount is low and red if it is high. The hotter the final color of the reagent, the
higher the concentration of reducing sugar.

The data shown above is an indication that fruit juice is low in carbohydrates when we
burn them in our body, as well as milk and milo. Therefore urinating has nothing to do
with burning or reducing carbohydrates or fats in the body.
PUTTING OF BENEDICT’S
IN THE TEST TUBE

PUTTING OF EACH
SAMPLES IN THE TEST
TUBES