Professional Documents
Culture Documents
An undergraduate thesis
presented to
Morong, Rizal
In Partial Fulfilment
Engineering
February 2022
CHAPTER 1
BACKGROUND
OF THE STUDY
flavoured milk as it enhances the flavour of the chocolate without making it harsh or
bitter, provides an overall smoothness and flavourable mouth-feel and extends its shelf-
life (Bixler et al., 2001). Chocolate milk is formulated with milk, cocoa powder, sugar and
hydrocolloids. The final composition, physical and sensory properties of the chocolate
milk largely depend on the levels of the ingredients including fat, the type of cocoa and
all UHT flavored milk to increase the creaminess of the final product and impart a more
lasting taste (Anonymous, 2000). The largest dairy application for kappa-carrageenan (j-
and provides long-term suspension of the cocoa particles (Bixler et al., 2001). The
Heat treatment significantly increases the shelf-life of flavoured beverages and also
aids the hydration of the hydrocolloid. It has also been shown that heating milk
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gels and improves the long-term stability of the product. This has been attributed to the
interactions which are largely responsible for formation of the weak gel network
However, during sterilization, problems may arise due to the increased viscosity
achieved by the hydrocolloids and also due to the interactions between the constituents
of the flavour and milk that result in flocculation, coagulation and sediment formation
(Ramesh et al., 1993; Tziboula and Horne, 2000). If the amount and type of stabilizer
are not satisfactory, the finished product can exhibit a number of undesirable
Traditionally j-car has been used as a stabilizer in protein systems, particularly dairy
carrageenan (k-car) (Zhang et al., 2004), suggesting the potential importance of k-car in
dairy processing.
(Folkenberg et al., 1999), rheological and optical properties (Yanes et al., 2002b). The
effect of heat treatment on the stability of chocolate milk has revealed three types of
light- and dark-coloured layers (Yanes et al., 2002b) that arise due to interactions
between the ingredients of the chocolate and milk components (van den Boomgaard et
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al., 1987). Fouling or deposit formation is an every-day concern of the dairy industry and
is a very common problem during UHT processing of chocolate flavored milk. No work
has been reported on fouling that takes place during UHT treatment of chocolate milk.
The main aim of the present work was to study UHT processing of chocolate milk with
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Chapter 2
Design
Methodology
models, and the statistical treatment which will be applied and utilized in
Reconstituted whole milk was prepared by mixing whole milk powder into water to a
final concentration of 120 g per L at 35 C using a heavy duty mixer (van den Bo
omgaard et al., 1987). Standard chocolate milk was prepared by adding cocoa powder
(1.5%), caster sugar (7%, 9% and 11%) and carrageenan (j-car or k-car) (0.00%,
powder was supplied by Frutex Australia (New South Wales, Australia). j-car (WG80 M)
was supplied by Woods & Woods Pty Ltd. (NSW, Australia) and k-car (Seakem CM611)
UHT Processing
A bench-top UHT plant, similar to the one described by Wadsworth and Bassette
(1985), was used in the experimental trials. The holding times in the preheating and
treatment the milk was cooled to 6 35 C in 25 s in a water-jacketed cooler. The flow rate
of the milk was maintained at 120 mL/min. The Reynolds Number (Re) of the flow was
calculated as 4.03 104 , which falls in the turbulent flow regime (Holman, 2002).
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The bench-top plant was instrumented with thermocouples located at the inlet and outlet
of the pre-heater and the outlet of the sterilizer and high-temperature holding sections.
The thermocouple at the outlet of preheater was at the inlet of the sterilizer section. All
Company Ltd., Taiwan) which measured and recorded inputs. The system used a PC-
based data acquisition system, VISIDAQ, that allowed real-time monitoring of data and
generated data logs which were used for data management in MS-Excel. Milk flow rate
was determined by measuring the time required to collect a known volume of milk.
Three measurements were recorded for every 5 min of the entire run and their average
Fouling measurement
(OHTC) which was measured using the following equation from Kastanas
(1996):
G cp∆θ
OHTC =
A ∆ T lm
where G = mass flow rate of the milk in kg/s; Cp = specific heat of chocolate
milk in J/(kg°C) – this was calculated taking into account the specific heat
capacity and mass fraction of each component (milk powder, specific heat
capacity = 3750 J/(kg°C) Walstra et al. (2005)); cocoa powder, specific heat
capacity = 1200 J/(kg°C) (per- sonal communication Dr. Ulrich Krause); sugar
specific heat capacity = 1250 J/(kg°C), Walstra et al. (2005). The specific heat
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capacity of carrageenan was not included as a negligible amount of
the inlet and outlet of the UHT section, in °C; A= heat exchanging surface area
of the tubing = 1.627 × 10—5 m2; DTlm = logarithm mean temperature difference
Where To is the temperature of oil in the high-temperature section in C; Tmo and Tmi
are the temperatures of milk at the inlet and outlet of the high-temperature section,
respectively (Kastanas, 1996). The bench-top UHT plant was operated and cleaned as
described by Kastanas (1996). The experiment was stopped when the temperature at
the outlet of the high-temperature section dropped below 120 C or earlier if deposits
blocked the channel or if the back-pressure could not be maintained at 0.4 MPa. The
indicator of the pressure gauge fluctuated from its set point of 0.4 MPa with fouling, and
the fluctuation became more pronounced as deposit built up on the walls of the tubes.
Apparent Viscosity
The apparent viscosity of the UHT-processed chocolate milk was measured at room
Sediment Measurement
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particles, under gravity. Sediment in the sterilized chocolate milk was measured
estimated by a centrifugation method, using calibrated centrifuge tubes (50 mL). The
weight of the centrifuge tubes was recorded and milk was weighed (40 ± 0.1 g) into
them. The samples were placed in a centrifuge (Model 5702 R, Eppendorf) at 3000g for
15 min. After centrifugation, the solid sediment was separated from the supernatant by
decanting. The tubes were then placed in an oven at 120 C for 36 h and the dry weight
of the sediment was measured. For each sample, duplicate analyses were carried out.