1.

An image analysis method for analyzing an image (78) of a

tissue collected from a subject using a deep learning
algorithm (60) of a convolutional neural network comprising an
input layer (50a), an output layer (50b) and an intermediate
layer (50c) configured by a plurality of layers, the image
analysis method comprising:
generating analysis data (80) from an analysis target
image (78) that includes an analysis target tissue by
generating a plurality of analysis data (80) for each region
in the analysis target image (78) having a predetermined
number of pixels, the analysis data (80) comprising, for each
of the predetermined number of pixels, color density value
data (81) for one or more primary colors;
inputting the analysis data (80) of each region having
the predetermined number of pixels to the deep learning
algorithm (60) in order to generate, for each region having
the predetermined number of pixels, a label value (83)
indicating that a pixel located at the center of the analysis
data of that region is a nucleus region and estimated value
indicating a layer structure of one of a plurality of layer
structures or that the pixel located at the center of the
analysis data of that region is a region other than a cell
nucleus; and
generating a layer structure nucleus region weighted
image (84) indicating the nucleus region of each layer
structure,
wherein the deep learning algorithm (60) is trained by:
first training data and second training data, and the
convolutional neural network learns the relationship between
the first and second training data,
wherein the second training data includes a label value
which indicates a nuclear region of a layer structure and the
label value is a value estimating the layer structure.
2. The image analysis method according to claim 1, wherein the
analysis target image (78) is an image of a sample for
histological diagnosis.

3. The image analysis method according to claim 1 or 2,

wherein
the analysis target image (78) is a bright field image
of a stained analysis target tissue.

4. The image analysis method according to claim 1, wherein the

plurality of layer structures comprises at least one selected
from an epithelial cell layer, an endothelial cell layer, a
connective tissue layer, and a muscle layer.

5. The image analysis method according to any one of claims 1

to 4, wherein
the tissue is taken from the stomach, small intestine,
or large intestine, and
the plurality of layer structures includes at least one
selected from among a mucosal epithelial cell layer, a mucosal
lamina propria, a muscularis mucosae, a submucosal layer, an
intrinsic muscle layer, and a serosal layer.

6. The image analysis method according to any one of claims 1

to 4, wherein
the tissue is collected from a trachea or a bronchus,
and
the plurality of layer structures comprises at least one
selected from among a mucosal epithelial cell layer, a mucosal
lamina propria, a smooth muscle layer, a submucosal layer.

7. The method according to any one of claims 1 to 4, wherein

the tissue is collected from the uterine body, and the
plurality of layer structures comprises at least one selected
from a mucosal epithelial cell layer, a mucosal lamina
propria, a muscle layer, an adventitia layer.
8. The image analysis method according to any one of claims 1
to 4, wherein
the tissue is collected from a gall bladder; and
the plurality of layer structures includes at least one
selected from a mucosal epithelial cell layer, a mucosal
lamina propria, a muscle layer, and a serosal layer.
9. The image analysis method according to any one of claims 1
to 4, wherein
the tissue is a glandular tissue collected from tissues
other than the stomach, small intestine, large intestine and
uterus, and
the plurality of layer structures comprises at least one
selected from a gland epithelial cell layer, a muscle layer,
and a connective tissue layer.
10. The method according to any one of claims 1 to 4, wherein
the tissue is a heart, and the plurality of layer structures
comprises at least one selected from among an endocardial
layer, a myocardium layer, an epicardial layer, and an
epicardial tissue.
11. The image analysis method according to any one of claims
1 to 4, wherein
the tissue is a blood vessel, and
the plurality of layer structures comprises at least one
selected from an endothelial cell layer and a muscle layer.
12. An image analysis apparatus (100) for analyzing an image
(78) of a tissue collected from a subject using a deep
learning algorithm (60) of a convolutional neural network
comprising an input layer (50a), an output layer (50b) and an
intermediate layer (50c) configured by a plurality of layers,
the image analysis apparatus (100) comprising:
a processor (10) configured to:
 generate analysis data (80) from an analysis target
image (78) that includes a tissue or cell to be analysed by
generating a plurality of analysis data (80) for each region
in the analysis target image (78) having a predetermined
number of pixels, the analysis data (80) comprising, for each
of the predetermined number of pixels, color density value
data (81) for one or more primary colors;
input the analysis data (80) of each region having the
predetermined number of pixels to a deep learning algorithm
(60) in order to generate, for each region having the
predetermined number of pixels, a label value (83) indicating
that a pixel located at the center of the analysis data of
that region is a nucleus region and estimated value indicating
a layer structure of one of a plurality of layer structures or
that the pixel located at the center of the analysis data of
that region is a region other than a cell nucleus; and
generate a layer structure nucleus region weighted image
(84) indicating the nucleus region of each layer structure,
wherein the deep learning algorithm (60) is trained by: first
training data and second training data, and the convolutional
neural network learns the relationship between the first and
second training data,
wherein the second training data includes a label value
which indicates a nuclear region of a layer structure and the
label value is a value estimating the layer structure.