Name: Tan Jing Wen

Student ID: J18026253

Section: B2

Experiment 4
Title: Techniques in Microscopy

Results:
I. THE COMPUND MICROSCOPE

Objective lenses

Lens Magnification
Scanning (small) lens 4x
Low-power (medium) lens 10x
High-power (large) lens 40x
Oil immersion (largest) lens 100x

Mixed Bacillus Gram Stained w.m. Mixed Bacillus Gram Stained w.m.
II. STEREOSCOPIC MICROSCOPES

Head of cricket Tail of cricket

Worm
Question:
1. In the table below, describe how the properties of the image change as you use different objectives
on your microscope.

Objectives
Image Properties
Scanning Low High
The magnification of the image increases from scanning
Magnification
objective to high objective.
Field of view Magnification increases, field of view will decrease.
Brightness of field Field of view decreases, brightness of field will also decrease.
Resolving power Resolving power will increase when the brightness decreases.

Problems encountered in the experiment

1. The image is hard to be focused using light microscope.
2. The body of cricket is not fully viewed through the stereoscopic microscope.

Solutions to overcome the problems

1. Adjust the location of the object with the knob of the microscope to get a clearer image.
2. Divide the image of cricket into two parts to be observed which is head and tail part.