Li@uizyme
CALCIUM
(arsenazo Ill Method}
Code_[ProductName Packie
Sonshine Coun ieverazemi) [251
£50388] Lgutqye Caicum serazeith | 807,
LS034¢ | Uizyne Colca isenaze i [700.1
0340 [ Lily Cal sera | 10 x100
S034 | Lcuizyme Calcium Prsenazo ty] 1450 ni
Ls034F [ igsnyme Cat fsenaze | 250"
[LS0386 igunyne Cau fsenaze i) | 79%50 mr
Intended Use
Diagnostic reagent for qunttave in wito deteminaton
lineal signiteanee
Calesmhasumerous uncon thin the body, nt only 28
‘structural far in bones and teeth, bu a nal
neuromoscltfunclonatste citing of 90d
Hypercalcemia may eevelop in patents with Pagers
«seas of bone ar hypeparatyrldsm, The cause of
Iypercaleaens in malgnancy ‘5 an increased bore
respon ei cased by melstss By hur
‘actors prducedby he tuner cel
In Rekets, Coeloe eseases, iopatle steatrhes,
‘esteomalaci, topical sprue and fellewing surgical
resection ofthe sl intestine, serum eau is oten
mederately reducee, usualy In asseciation wth low
plasmaprotenconcetation
Principle
rsenazoll combines wih eeu ion at pH 6 Storm a
colored cremaphore, the absorbance ef wich is
measured a 650 rn (680-660 nn ands proportional
‘alum concent,
‘snare has high affnity (C= 1x10 fr eae ons
Sand sos no interference rom other estions nelly
bresertinseram plasma une.
Reagent Composition
Reagent: Callum Reagent
rsenazo 010 nme
bute > 50mm
Reagent 2: Cael Standard
Ready tose
sami
Reagent Preparation
Reagents ae que ready 10456
Motels Requed But Net Provided
ean & ry contain
Laboratory lssPippetesfNiapppetes Tis
ColemeterarBo-ChemistyAralaet,
BEACON
Stability Andstorage
“The unopened reagents ae sable ti the expy date
stnled onthe Bote ae bel wen store 3t Room
temperature
‘Specimen Callecon AndHnling
Use unheamolyse serum,
Is ecommendedo ftow NCCLS process fr snlor
sancardzee conditions.
Stab In Serum
Tass at20-25°¢
awe sure
Events stave
Catiratin
Chbratoniththe Colm slandre provides hes
recommended
ually Control
Iestecommended oun normal and abnormal cen! sera
tovalcatereagentperermance
Unit conversion
rmgidx025=mnel
ExpectedVaues
serum a5stonsid
Ins ecommended that enehlaboratary very this ange
or derverreferenceinteratorthe populstontzerves,
Performance Data
Dita contained wth tis secton x representative of
performance ondeacensyem,
Dita ebtiined in your aborstary may ete rom these
Precision
Inte assaypretiion] Mean | SD] eV
wininran 20) | gga | ire | 0)
Sample rs a
Sample 7 | on | 095
interassay precision] Wean | SD [cv
Runtoruniy-20) | gic | iia | 6)
Sample? ax | oo | on
Comparison
2 comparison between Ligunyme Caléum fy) and 2
omercaty avaiable test) using 20 samples gave
follwingresuts
y= 09825-0138
r= 0985
“BEACON DIAGNOSTICS PVT. LTD. 42, WEW GIDC, KABILPORE,WAVSARI- 986.428 NDIAInterterences References
Following substances dontinertre 1.8ihep,M.C OUBEBYON LAUFEN, JL, car Aa
haemagiooin up to 10 gi, biltubin up to 4Omgrd, _aavweod. Tet 10.81
teerides upto 500 mg
Waring AnaPrecautions
For invita eagnetc se. Tobe had by elie ant
profesanalyeduestedpeson
Reagentsrtnektaronatclsied ike dangerous
Waste Management
Pease refertolcalegalrequrerets
Assay Procedure
Wavelength 200m
cwette tem
Pipette into clean ery test tubes labeled as Blank
Standre(s and Test:
adivon Sequence [Reagent Bark [Siandare | SE
Reagent 10003 | 1000! | 1000p
‘Sancard 20
Same 2H
Ditied Wier 2
[Micand incubate at for 2 in. Messe absorbance of
tanga os) atest Abs, T aginst reagent ink
3t6300m,
Caleulton
Abs.
Caléum fmgia}= — 10
Boss
‘Applications for automatic analysers
onrequest.
Assay Parameters For Photometers
PS ee] 8 Montscare
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