@uizyme, UREA (NED method) Code ProductName Packs Tsuen | Tamme Deo NED need! [foo Intended Use Diagnostic reagent fr quantative nvr deterination ofUreain human serum andl Cina sigitcance ress the end product of protein metabolism, IIs _yhesedin the ver fom the amma preeiced by tne CGtabolsm of amino ald tis Wansprtd bythe Blood tothe kdoey rom wheres exceed ineesedlevels are feund In real elsease, unary abstuctons, shock congestive heat faiure and utr. Decree levels are foundiniveralureand pregnancy Prince reas an acemeciam condenses wtho-phtaliehyde snd nepty! tylene Diamine o form a eolre complex. he fate of formato of ths camper mesure a6 an increase insbseranceinafixtme wich sraporbonslto thewreaconcenrateninthesanple, Reagent Competition Reagent 1: OPA Reagent ont >i nmol Reagent 2=NED Reagent NED at nmol Reagent 3 Uren Standard Ready touse Materials Requred But Not Proved ‘lean ry conti Labratry Glass PipetesorMicploctis Tis Colrineteorio Cristy Anny Reagent Preparation Reagent isiqu,reaeyto use Sabi And Storage he unopened reagents ate stable tl the expiry dite tele th botlesnchtiabelwnen treet 28°C Someia Specimen Collection And Hneling Use unheamegte serum or plasma fhepan, EDTA and sie. is recommen ofl NCCLS praceces or simiarstandardizes conten) Stabity Seays a2-8¢ Dice contaminated specimens. Calibration Caltraton withthe Utea standard provided in the kts recommenced B ualty Conta! Itstecommended run nomal and abner! cent! sera tovabatereagentperormance Expected Values Serum 15-50gie Ins recommended that enchlaboratory vey this ange or derives referenceintera forthe population tzerves Performance Dats Data contained wth tis secton is represeratwe of performance on Beacon system. Data obtained in your laboratory may er omthesvalues. Limit of quanteation : tg Lnesiy 200 nei Messuingrange 1-200 mg Precision nie assay preeiion] Mean | SD] Ov itninran 20) | gmgien | dey | 0) Sample 2 [os [2s “Some? ne [is [tat interassay preasion] Ween | SD |v Run toran 20) | ¢mgied | dnote | _¢) ‘Sample [080 [208 Comparison 1 comparison between Urea i) and 2 commercially avalabletestpusing 20 samples gavetlowingresuits: y= osezx-0182 + = 0987 Warning AndPrecowtons Fer in tro dagnesc use. Tobe handle by eile ane petessonaly educates serson, Waste Management Peasretrteocallealrequiement Assay Procedure Wavelength 505 nm wets ten Tailor Sequence | Sandord | Sample Reagent 70003 7000 ‘Sunde soul Sample sou Reagent? 5004 5004 BEACON DIAGNOSTICS PVT. LTD. 44, NEW GIDG, KABLPORE, HAVSARI- 396.424 NDIAMxwetaneresethe ina absorbamce Aor he staneard nates ater exact 60 second Read arses absorbance ‘cote stndar ane test exact 60 secon tera 505 in. alate he change in absorbance bath stanearé rates Caleulation oe. rea ita x50 os. Assay ParametersForPhotometers ose Fred Tine Wavelength | 505 Sample Volume ih 30 Reagent 1 700 Reagent 2 50048 Tagine ec) 0 Reaitine Sec} a Tresbaten temp.) v Nona Loe gl = Norma Figh al 30 neariyLow inh t TDreory High al 700 ‘SiandrdConcenrson sono Ua naa References Comal, A, Bacal C.J, David, MM: J Bi chem 7,751,198, 2.doumas,B.1, Bayse, O.0akel: Cin. Chem. 27, 1642, 198 S.chromy, V, Flscher, 1: Clin. Chem. 23, 758, 187 “Crom. Viste) Nemieek. J: Z Me Labor Diagn 21 33,1880 5 Tietz Textbook of Cina Chemisty ond Matecuat sdagnests. urs, CA. 6 Ashwood, ER, Bruns, DE; St eden, We Seunders ‘Symbols Used On Labels ee] SI a aoutecuer Ci ie ] vonove [RO]. nvr ongnosts let