RF TURBILATEX Code SEO2SR| Product Name RF Turbine Pack ae Son ‘Quantitative determination of Rheumatod Factors (RF) iy Sioreat26¢ Principle oT Method TheRFTubiateris2quantatveturidmetic tes orthe rmeasiromentofRFinhamansenenor plasma Liter paces costes wih human garimaglobul are sggutnated wen ized with samples contaiing RF. The Spputnston caures an abserbance charge, cependent "upon he AF comets of sample hat canbe gua by cerpaison roma calraerof oun concent, Cina signiiance Rneumatlé acters ae a group of annie dreced to determinants nthe Fe potion of the inmunogibulin & rmolecule.Atough rheumato ‘acters ae fund in 2 umber of heumataidesorde's, such as stem ips eytematesus (SLE ane idre's syndrome, 25 welas in roneheumateeoneans, seen ae In ee ess tly asan ain tne agnosis fmeumatid artis RA Astuay tthe American Colege of Rheumatley” shows Uhattheg0 4% e1RApatents ere postive Tisbate 0 ma Reagent: Divert Preservathe, (Blox pares coated win garimapabu, Presenatne Reagent 2: Latex Antigen ‘albrator The RF Regent 3:RF Calibrator | concenration states onthe val abel Precautions Components far hunan etgh have Been teste ane found tobe negative ote presence of HEstg, HCV and antbody te V2 However hand cautious aspetenty infectious Catiration Use RF Calratr Proved wth kt. The senatty of te assay an the target val of the carat have been standarized against be ntenatonl Reference Sancaré ‘rom NIBSC 64/02, Recairate when conte resus are lout of specied tolerances, when sing ciferet lt of Teagentandwhen he instruments aused Preparation AF Calloator: Resa ture, B Caran Curve: Prepare the foloning RF caltater suas nNoC! 9 i, Muti the concentration a he RF Calbretor by the coverpendng factor sted in table besentocblntheRF encentatoneteveh von, [riborenton] 1] 2 [2] *[ 5] 6 CarterRr by | —|_25 | 80 | 700 [200] 200 WaCt sgt [A00] 375 | 350 | 300] 200 [50625] 725 | 075] 05 | To Feo [0 Storage And tbiy [Alte component othe kare stable unt the exaation ae onthe lab! wen tred tly cosed 324°C are Contamination prevented dng ther use. Do net use reagertsovertheexpirtondate Reagent Deterioration: Presence efartcles natu, Reconstitte Calibrator: Sadie or I ath at 28°C 03 monte 20°, De nat eeze; zen Latex or Diuent cou change the functonaltyofthetes, ‘Addional Equipment Thermestate bath st 27, Spectiophotomete or photometer thrmestatable at _PCwih a 6S0nm he, samples Fresh seu, sabe 7 aay 28°C 6 Smet at. 20°C Samples wt presence ef fvinsheuld be centfugee berate testing Do not use Nighy Memolzes © ipemie samples. Procedure "Bring the reagent and photometer feet Nader) to are 2.Bssay conden: Wavelength «550nm 600-650) Temperature are Ccwtelgthpath tem 4 Agustheinsrumerttezerwithdistied wate 4 Pipeteimaacavete Ton Sequence ane Divert 3009 Toten 2 00H ‘SMix and read the absorbance (Blank reagent) 6 Acdtresamplecalate: “BEACON DIAGNOSTICS PVT. LTD. 42, WEW GIDC, KABILPORE,WAVSARI- 986.428 NDIAmesetun | 7 Talbstorer amp BL) 7 Galbrior Sompe "x and ed te absorbance ater 2 minstes (A fhe samgieaeiton Cateultons Calesate the absorbance deren (Kenan Of €8¢h point ofthe ealvation curve and plot he ales ebtaied against the RF concenratan ofeach easter dion Rhewmater factor concentration in the sample is calelsted by interpolation of RF (A-Ags nd I the calistoncarve Quality Contra Conta era ae recorimende te mantorthe performance ofmonuolaneautomatesassy precedes, Esch aborstey shoud establen is on Quay Conol ‘heme and crrecve acon i conrls do nt mest the accptabletlerarces. Reference Values Noinsvaues vato201Uin. Each aseratory shoul stabs ts own reference range Perormance Characteristics ‘Limi detection: Vues les than 6 [In give non rexroducleresuts _2Mearurement range: 6:60 WinL, under the descbed! tssay contions. Samples wth higher cencetatiens Should be dute U5 in NaCl 9 gL ane retested again. The ineay bmi and measurement range éenenes on the sample to reagent, as well as the analyzer sed. tilde higher by ceceasing the sarpevelume, although the senstivty ofthe est willbe prepetonaly tecescee a Presone eect No prozone tec was deleted upon ‘Theresuts ofthe perfomance characters dependon theanaeruse, Inerterences Hemoglobin 0 gl}, iin (20 mg and pei 0 Si), danotntertere Ohersuastancesmay rere Notes Cincl eiagnoss stould not be made on fangs of single test esl, but shoul integrate beth clic! ane laderatryasta Bibliography “LFrecrick Welle etal. Arts and Rheumatism 18834 ‘95-960 Rober W Derner at al, Clinica Chimica Acta 1987, er 121 Rober Hi Shmeting et a Medicine 99% 91528 — 524, 44 Vladimir Mulé et, Seand J Rheumatology 1972: 1 eter Paul R et al, Chn Chem 1879; 25/1 1909 — 114 65 Young. eects of ergs once aberatory test th eA AACC Pres, 195, he Amesean Jounal oot Sent 3 348 Ui Shrecson Ta meng [ws | 3 Ge [FA] Sot Und ontabet wv a 23 var] aoie sete kona tl ts Tay Fo free Tenis se) OR) SS ae] terns 3 past Accuracy: Ress ebtainee using ths reagent were ompavediothose atanadsing a conmercileagent bi with ler enaraceritcs, 86 samples ranging er 10160 lun. of BF were assayed. The carectsion ceetfcep wae 095 ane tre regression equation y o7e7et075, YZ Gorvose Eww] nv Dagrastes ele