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  • Macrogen RNA QC Criteria

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    waheed akram
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    This document provides quality control criteria for various RNA sequencing library preparation kits, platforms, and library types. It includes information on concentration, volume, total amount, RNA integrity, rRNA ratio, DV200, and viability for each library preparation. Most of the libraries have a concentration of 0 ng/ul, volume of 0 ul, total amount of 0-1 ug, RNA integrity and rRNA ratio of 0%, DV200 of 50-70%, and viability of 70-100%. PacBio Iso-Seq libraries for the RS II and Sequel platforms aim for total amounts of 2-3 ug, RNA integrity of 7, and other metrics similar to the other libraries.

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    This document provides quality control criteria for various RNA sequencing library preparation kits, platforms, and library types. It includes information on concentration, volume, total amount, RNA integrity, rRNA ratio, DV200, and viability for each library preparation. Most of the libraries have a concentration of 0 ng/ul, volume of 0 ul, total amount of 0-1 ug, RNA integrity and rRNA ratio of 0%, DV200 of 50-70%, and viability of 70-100%. PacBio Iso-Seq libraries for the RS II and Sequel platforms aim for total amounts of 2-3 ug, RNA integrity of 7, and other metrics similar to the other libraries.

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    © All Rights Reserved
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    25 views3 pages

    Macrogen RNA QC Criteria

    Uploaded by

    waheed akram
    This document provides quality control criteria for various RNA sequencing library preparation kits, platforms, and library types. It includes information on concentration, volume, total amount, RNA integrity, rRNA ratio, DV200, and viability for each library preparation. Most of the libraries have a concentration of 0 ng/ul, volume of 0 ul, total amount of 0-1 ug, RNA integrity and rRNA ratio of 0%, DV200 of 50-70%, and viability of 70-100%. PacBio Iso-Seq libraries for the RS II and Sequel platforms aim for total amounts of 2-3 ug, RNA integrity of 7, and other metrics similar to the other libraries.

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    © All Rights Reserved
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    of 3

    RNAQCCriteria

    Platform LibraryType LibraryKit Type Conc. Volume TotalAmount Rin rRNARatio DV200 Viability
    (ng/ul) (ul) (ug) (%) (%)
    TruSeqRNAAccess FFPERNA 0.0 0.0 0.1 0.0 0.0 50.0 0
    TruSeqRNAAccess TotalRNA 0.0 0.0 0.1 0.0 0.0 50.0 0
    ChromiumSingleCell3pRNALibraryv2 TotalRNA 0.0 0.0 0.0 0.0 0.0 0.0 70
    ChromiumSingleCell3pRNALibraryv2 mRNA 0.0 0.0 0.0 0.0 0.0 0.0 70
    ChromiumSingleCellVDJLibraryv1 TotalRNA 0.0 0.0 0.0 0.0 0.0 0.0 70
    ChromiumSingleCellVDJLibraryv1 mRNA 0.0 0.0 0.0 0.0 0.0 0.0 70
    HiSeq2500 TruSeqmRNA(Microbe) TotalRNA 0.0 0.0 1.0 7.0 1.0 0.0 0
    HiSeq4000
    HiSeqXten TruSeqStrandedmRNA(Microbe) TotalRNA 0.0 0.0 1.0 7.0 1.0 0.0 0
    MiSeq mRNALibrary
    SureSelectRNADirect_Human TotalRNA 0.0 0.0 0.1 0.0 0.0 50.0 0
    NovaSeq
    NextSeq500 SureSelectRNADirect_Human FFPERNA 0.0 0.0 0.1 0.0 0.0 50.0 0
    SureSelectRNADirect_Mouse TotalRNA 0.0 0.0 0.1 0.0 0.0 50.0 0
    SureSelectRNADirect_Mouse FFPERNA 0.0 0.0 0.1 0.0 0.0 50.0 0
    ChromiumSingleCell3pRNALibraryv3 TotalRNA 0.0 0.0 0.0 0.0 0.0 0.0 70
    ChromiumSingleCell3pRNALibraryv3 mRNA 0.0 0.0 0.0 0.0 0.0 0.0 70
    TruSeqmRNA TotalRNA 0.0 0.0 1.0 7.0 1.0 0.0 0
    TruSeqmRNA(InsertSize) TotalRNA 0.0 0.0 4.0 7.0 1.0 0.0 0
    Platform LibraryType LibraryKit Type Conc. Volume TotalAmount Rin rRNARatio DV200 Viability
    (ng/ul) (ul) (ug) (%) (%)
    TruSeqStrandedmRNA TotalRNA 0.0 0.0 1.0 7.0 1.0 0.0 0
    SMARTerUniversalLowRNALibrary TotalRNA 0.0 0.0 0.05 7.0 1.0 0.0 0
    SMARTerUltraLowInputRNALibrary TotalRNA 0.0 0.0 0.01 7.0 1.0 0.0 0
    SMARTerStrandedRNALibrary TotalRNA 0.0 0.0 0.05 7.0 1.0 0.0 0
    mRNALibrary TruSeqmRNA mRNA 0.0 0.0 0.1 0.0 0.0 0.0 0
    TruSeqmRNA cDNA 0.0 0.0 0.1 0.0 0.0 0.0 0
    HiSeq2500 TruSeqStrandedmRNA mRNA 0.0 0.0 0.1 0.0 0.0 0.0 0
    HiSeq4000
    HiSeqXten TruSeqmRNA ExosomalRNA 0.0 0.0 0.01 0.0 0.0 0.0 0
    MiSeq TruSeqStrandedmRNA ExosomalRNA 0.0 0.0 0.01 0.0 0.0 0.0 0
    NovaSeq
    NextSeq500 TruSeqTotalRNAwithRibo-ZeroBacteria TotalRNA 0.0 0.0 1.0 7.0 1.0 0.0 0
    TruSeqStrandedTotalRNAwithRibo-ZeroH/M/R TotalRNA 0.0 0.0 0.5 7.0 1.0 0.0 0
    TruSeqStrandedTotalRNAwithRibo-ZeroH/M/R_Gold TotalRNA 0.0 0.0 0.5 7.0 1.0 0.0 0
    TruSeqStrandedTotalRNAwithRibo-ZeroH/M/R_Globin TotalRNA 0.0 0.0 0.5 7.0 1.0 0.0 0
    TotalRNALibrary
    TruSeqStrandedTotalRNAwithRibo-ZeroPlant TotalRNA 0.0 0.0 0.5 7.0 1.0 0.0 0
    TruSeqStrandedTotalRNAwithRibo-ZeroBacteria TotalRNA 0.0 0.0 1.0 7.0 1.0 0.0 0
    iR-Profile_ShortReadLibrary TotalRNA 0.0 0.0 0.05 7.0 1.0 0.0 0
    TruSeqStrandedTotalRNA(NEBMicrobe) TotalRNA 0.0 0.0 1.0 7.0 1.0 0.0 0
    Platform LibraryType LibraryKit Type Conc. Volume TotalAmount Rin rRNARatio DV200 Viability
    (ng/ul) (ul) (ug) (%) (%)
    TruSeqTotalRNA(NEBMicrobe) TotalRNA 0.0 0.0 1.0 7.0 1.0 0.0 0
    TotalRNALibrary TruSeqTotalRNAwithRibo-Zero TotalRNA 0.0 0.0 1.0 7.0 1.0 0.0 0
    TruSeqTotalRNAwithRibo-Zero(Insertsize) TotalRNA 0.0 0.0 1.0 7.0 1.0 0.0 0
    NEBNextSmallRNALibrary TotalRNA 0.0 0.0 1.0 7.0 1.0 0.0 0
    HiSeq2500 TruSeqSmallRNALibrary TotalRNA 0.0 0.0 3.0 7.0 1.0 0.0 0
    HiSeq4000
    HiSeqXten TruSeqSmallRNALibrary SmallRNA 0.0 0.0 0.1 0.0 0.0 0.0 0
    MiSeq SMARTerSmallRNALibrary TotalRNA 0.0 0.0 0.05 7.0 1.0 0.0 0
    NovaSeq
    NextSeq500 SmallRNALibrary SMARTerSmallRNALibrary SmallRNA 0.0 0.0 0.01 0.0 0.0 0.0 0
    NEBNextSmallRNALibrary SmallRNA 0.0 0.0 0.1 0.0 0.0 0.0 0
    TruSeqSmallRNALibrary ExosomalRNA 0.0 0.0 0.01 0.0 0.0 0.0 0
    SMARTerSmallRNALibrary ExosomalRNA 0.0 0.0 0.01 0.0 0.0 0.0 0
    NEBNextSmallRNALibrary ExosomalRNA 0.0 0.0 0.01 0.0 1.0 0.0 0

    Platform LibraryType LibraryKit Type Conc. Volume TotalAmount Rin rRNARatio DV200 Viability
    (ng/ul) (ul) (ug) (%) (%)
    RSⅡ PacBioRSIIIso-SeqLibraryConstruction TotalRNA 0.0 0.0 2.0 7.0 1.0 0.0 0
    PacBioLibrary PacBioSequelIso-SeqLibraryConstruction TotalRNA 0.0 0.0 2.0 7.0 1.0 0.0 0
    Sequel
    PacBioSequelIso-SeqLibraryConstruction(+4kb) TotalRNA 0.0 0.0 3.0 7.0 1.0 0.0 0

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