Instrumental Analysis Chemistry

it must be stable, pure and not exist in the initial sample
it Must be measurable, giving an elution peak well resolved on the chromatogram
its retention time must be close to that (or those) of the solute(s) to be quantified
its concentration must be close to, or above that of the analytes to quantify to gain
response from the detector
a linear

it must not interfere and co-elute with a sample component.

Problems
6,1) Explain why a Component in a sample placed
on a chromatography column
spreads out in a band as it moves through the column.
6 . 2) In chromatography,what is plate height? Is a larger or smaller plate height
desirable? Explain.
6.1) In a chromatographic analysis of low-molecular-weight acids, butyric acid elutes
with a retention time of 7.63 min. The column's void time is 0.31 min. Calculate
the capacity factor for butyric acid. (Ans: 23.6)
6.4) In the same chromatographic analysis for low-molecular-weight acids considered
in 6-3, the retention time for isobutyric acid is 5.98 min. What is the selectivity
factor for isobutyric acid and butyric acid? (Ans: 1.29). .
6.5) A chromatographic analysis for the chlorinated pesticide DieIdrin gives a peak
■vith a retention time of 8.68 min and a baseline width of 0.29 min. How many
theoretical plates are involved in this separation? Given that the column used in
is is 2.0 meters long, what is the height of a theoretical plate? (Ans:0.1
this analysis 4
mm)
6. of benzene, toluene,
6) A mixture a nd m e t h a n e w a s i nj e c t e d i n t o a g a s
chrom.atograph. Methane gave a sharp spike in 42s, whereas benzene required 251
s and toluene was eluted in 333s. Find the adjusted retention tinge capacity
factor for each solute. Also, find the relative retention of the two solutes. (Ans: a =

1.39)
data were obtained for four compounds separated on a 20-in
6.7) The following
capillary column.
t r OW w (min)
ConlPound 8.04 0.15
A 8.26 0.15
13 8.43 0.16
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