Instrumental Analysis Che

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Beii Xuan Vali

(a)Calculate the n
number of to plate
for the column.
(b) Calculate the
average hei ght of a the
plate.

(c)Explain why it is

possible for each

compound to have a

different nu m b e r o f
 theoretical plates.
a component
placed on a 15-m
colu m n i f
column.

-n a 30_rn
6.8) Calculate the plate
height.gn ram)
has a retention
time of 2.41
minutes and a
baseline peak
of0 How
width Akan
many plates for the

component are

associated with

this column?

6.9) Consider the

following

chromatography data for

components sep ar at ed

a)Calculate the retention

factor for component A.
b)Calculate the
resolution for
components A and B.
Do these peaks
overlap to a
significant extent?
Explain.
6.10) A mixture placed in
an Erlenmeyer flask
comprises 6mL of silica
gel and 40mL of
a solvent containing, in
solution, 100mg of a
non-volatile
compound. After
stirring, the mixture was
left to stand before a
10mL aliquot of the
solution was
extracted and evaporated
to dryness. The residue
weighed 12mg.
Calculate the
adsorption
coefficient, K =
Cs/C M, of the
compound in this
experiment.
6.11) Moody studied the
efficiency of a GC
separation of 2-
butanone on a dinonyl
phthalate column.
Evaluating the plate
height as a function of
flow rategave a van
Deemter equation for
which A is 1.65 mm, B
is 25.8 mm x mL x min-1,
and C is
0.0236 mm x min x m1;1.

(a)Prepare a graph of
H versus it for flow
rates in the range of 5-
120 mL/mins
(b)For what range of
flow rates does each
th e
g re at est
effe c t?
 in the van Deemter
equation have
(c)What are the
optimum flow rate
f l o w
r a t e ?
the height of a
theoretical plate at
that