Temporary Immersion Bio-Reactor (basic DIY diagram)

Timer AC
Power

Syringe Filter PTFE

Membrane 25mm Syringe
Filter PTFE0.22μm,HPLC
Air Pump
Standard
aquarium Vent to atmosphere
type vinyl
tubing
3/16 ID 5/16 OD food /
medical grade silicone
rubber hose
(autoclavable)

Automotive grade
RTV silicone sealant

When air pressure is

applied media is
Humidifier forced up into growth
chamber, then slowly
Bath dripping back into
reservoir via cotton
weep wick.

Holes for tubing must

be melted with
chucked end of
appropriatesized
heated drill bit
ZipLock brand 1qt poly propylene
(#5) round food container with Liquid media
screw on lid / 2 containers double reservoir
stacked for growth chamber – media
reservoir sealed with RTV silicone
constructed in such a way these 2 ~1/8 inch cotton cloth weep to
containers can easily separate for drip media back into reservoir
cleaning

This is a basic diagram of the homemade DIY Bio-Reactor created in the style of the “RITA TIB”
This diagram is intended to be part of a complete DIY manual.
This diagram, the accompanying manual, and the DIY Temporary Immersion Bio-Reactor is an
original work by Keven Wilstead 2020, published with the intent to help anyone that may want to
experiment with a bio-reactor, but not to be used, or produced commercially without writen permission
from the creator.
kevenwilstead@gmail.com