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REVIEW
New Investigator Review Award
The differential role of reactive oxygen species in early and late stages of
cancer
Mohamad Assi
Laboratory “Movement, Sport and Health Sciences,” University of Rennes II-Ecole Normale Superieur Rennes, France
Submitted 27 June 2017; accepted in final form 20 August 2017
Assi M. The differential role of reactive oxygen species in early and late stages
of cancer. Am J Physiol Regul Integr Comp Physiol 313: R646 –R653, 2017. First
published August 23, 2017; doi:10.1152/ajpregu.00247.2017.—The large doses of
vitamins C and E and þ-carotene used to reduce reactive oxygen species (ROS)
production and oxidative damages in cancerous tissue have produced disappointing
and contradictory results. This therapeutic conundrum was attributed to the double-
faced role of ROS, notably, their ability to induce either proliferation or apoptosis
of cancer cells. However, for a ROS-inhibitory approach to be effective, it must
target ROS when they induce proliferation rather than apoptosis. On the basis of
recent advances in redox biology, this review underlined a differential regulation
of prooxidant and antioxidant system, respective to the stage of cancer. At early
precancerous and neoplastic stages, antioxidant activity decreases and ROS appear
to promote cancer initiation via inducing oxidative damage and base pair substi-
tution mutations in prooncogenes and tumor suppressor genes, such as RAS and
TP53, respectively. Whereas in late stages of cancer progression, tumor cells
escape apoptosis by producing high levels of intracellular antioxidants, like
NADPH and GSH, via the pentose phosphate pathway to buffer the excessive
production of ROS and related intratumor oxidative injuries. Therefore,
antioxidants should be pro- hibited in patients with advanced stages of cancer
and/or undergoing anticancer therapies. Interestingly, the biochemical and
biophysical properties of some poly- phenols allow them to selectively recognize
tumor cells. This characteristic was exploited to design and deliver nanoparticles
coated with low doses of polyphenols and containing chemotherapeutic drugs into
tumor-bearing animals. First results are encouraging, which may revolutionize the
conventional use of antioxidants in cancer.
cancer stages; reactive oxygen species; metabolism; genetic mutations; antioxidants
Initiation/promotion MNU-induced hepatocellular Papaya extracts With MNU-Treatment 1 Body weight; 2 Anemia, 1 SOD, CAT and GPx activity; 2
carcinoma in mice (61) inflammation and liver dysplasia DNA damage
B(a)P-induced lung carcinoma in Chrysin Before B(a)P-treatment 2 Tumor incidence and 1 SOD, CAT and GPx activity; 2
mice (32) proliferation of alveolar COX-2 and NF-nB expression
epithelium
PhIP-induced prostate cancer in a, þ, μ, and 6 Tocopherol Before PhiP-treatment 2 Prostatic intraepithelial neoplasia
1 PTEN expression; 2 P-Akt
mice (9) expression and 8-oxoG content
DMH-induced colon cancer in Quercetin With DMH-treatment 2 Colon dysplasia and inflammatory —
mice (52) cell infiltration
ROS IN
Low-grade (AH1) prostate Pomegranate jus With tumor implantation 2 Tumor size and proliferation 1 Bax/Bcl-2 ratio and cleaved
tumor-bearing rats (20) caspase-3; 2 P-ERK and 8-
oxoG content
Progression/metastasis/ Hepatocellular carcinoma Diterpenoid Isoforretin A: After tumor injection 2 Tumor volume 1 8-oxoG and DNA fragmentation;
cachexia (HepG2) tumor-bearing thioredoxin inhibitor 2
mice (62) GSH content and thioredoxin activity
High-grade colon (C26) tumor- Vitamin C and E,
bearing mice (2) quercetin, and With tumor injection 1 Cancer cachexia; 2 Survival 1 Ki-67 (proliferation); 2 4-HNE
curcumin content
Mice bearing human mammary Inhibition of glutathione
tumors xenografts (25) and thioredoxin After tumor transplantation 2 Malignant mammary tumor growth 2 Nrf-2 signaling
Mice bearing human melanoma NAC After tumor transplantation 1 Metastasis in blood and Metastatic nodules: 1 NADPH
xenografts (43) visceral organs generation via folate pathway
Mice Tyr-Cre LSL-BRAFCA/+ Trolox and NAC After tumor induction 1 Lymph node metastasis Metastatic nodules: 1 GSH/GSSG
PTENfl/fl (melanoma) (36) ratio
Mice iCre LSL-KRASG12D and Vitamin E and NAC After tumor induction 1 Lung cancer progression; 1 BrdU (proliferation); 2 p53
mice BRAFV600E (lung cancer) 2 expression and 8-oxoG content
(55) survival
AO, antioxidants; DMH, dimethylhydrazine; MNU, N-methyl-N-nitrosourea; B(a)P, benzo(a)pyrene; PhiP-P, 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine; NAC, N-acetylcysteine; PTEN,
phosphatase TENsin homolog; SOD, superoxide dismutase; CAT, catalase; GPx, glutathione peroxidase.
ROS IN CANCER R649
pathway to synthesize NADPH and regenerate GSH (51). zymes in tumor tissue appear to decrease during neoplastic
Consequently, NADPH and GSH buffer OS and allow cells to stages but increase in more advanced and malignant stages (6,
survive. Mechanistically, malignant cancer cells produce ex- 17, 27, 34, 53, 55) (Table 2). Similarly, inflamed tissue, a
cessive levels of H2O2 near the toxicity threshold; thus, giving precursor condition for cancer development, exhibits a de-
an antioxidant like N-acetylcysteine (NAC) reduces OS and crease in antioxidant capacities comparing to cancerous tissue
promotes proliferation (35). Panieri and Santoro (44) have in which antioxidants are highly present (60). Globally,
recently reviewed and listed the impact of some agents target- clinical observations and preclinical data suggest a tumor-
ing the tumor antioxidant defense. They found that depleting protective role for antioxidants in late stages of cancer.
NADPH and GSH enhanced the susceptibility of the malignant ROS-mediated tumor suppression routes. Mitochondrial
colon, ovarian, and lung cancer cells to ROS-mediated apopto- dysfunction and ROS accumulation are important proapoptotic
sis (44). This is in line with our research and that of other events occurring in cancer cells (71). They lead to the expres-
laboratories, showing that vitamins C and E, NAC, and quer- sion of the cell cycle inhibitor p53, cell cycle arrest at the
cetin accelerated cancer progression, metastasis, and cachexia G2/M phase, DNA fragmentation, and subsequent apoptosis
development in rodents, while inhibitors of thioredoxin and induction (71). Early in vivo observations demonstrated that
GSH induced tumor regression (2, 25, 36, 45, 57, 64) (Table 1). ROS can trigger apoptosis through increasing lipid, protein,
Clinically, the expression and activity of antioxidant en- and DNA oxidation within the tumor itself (72). Recent
zymes, as well as GSH content, are increased in malignant studies have confirmed that chemotherapeutic agents, like
tumors comparing to adjacent normal tissue (30, 46), and a cyclophos- phamide, increase ROS-induced lipid peroxidation
higher ratio of both levels (malignant/normal) is associated and apopto- sis in sarcoma-180 tumor tissues (29).
with poor survival (30). Interestingly, in melanoma, breast, and Mitochondria-dependent apoptosis is the most studied path.
ovary cancers, the expression and activity of antioxidant en- Excessive levels of ROS induce the oxidation of cardiolipin
(phospholipids), which, in
Table 2. Clinical studies demonstrating that the expression/activity of antioxidant enzymes and GSH content increase in
malignant tumors comparing to benign hyperplasia or precursor lesions
Patient
Type of Cancer Number Malignant Versus Benign or Precursor Events Antioxidant Defense Detection Method
Thyroid (51) 34 Carcinoma vs. adenoma 1 SOD, CAT, and GPx activity Colorimetric method
Ovary (17) 26 Malignant vs. benign 1 GSH content Colorimetric method
Breast (17) 26 Malignant vs. benign 1 GSH and GPx activity Colorimetric method
Prostate (6) 27 Cancerous vs. neoplastic 1 CuZnSOD expression IHC
Breast (34) 50 Stage III vs. stage I/II 1 SOD, CAT, and GPx activity Colorimetric method
Skin (53) 36 Melanoma vs. actinic keratosis precancerous lesions 1 CuZnSOD, MnSOD, and CAT IHC
expression
Pancreas (58) 13 Cancerous vs. pancreatitis 1 MnSOD expression WB
Ovary (27) 29 Carcinoma vs. benign cystadenoma 1 CuZnSOD and MnSOD expression WB and IHC