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1.

5 Apparatus for measuring

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measuring cylinders

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volumetric flasks

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There are various sizes of measuring cylinders and volumetric flasks ranging from 5 cm³ to 1 dm³.
Volumetric pipettes are made in only a few sizes, the most common being 10 cm³ and 25 cm³.
A burette is used to accurately deliver up to 50 cm³ of liquid.

1 dm³ = 1000 cm³ 1 dm³ = 1000 cm³

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When we measure out volumes of liquids, we need to think
about the accuracy required. A burette or a volumetric pipette
is much more accurate than a measuring cylinder. The scale
divisions on a burette can be read to the nearest 0.1 cm³ but a
100 cm³ measuring cylinder might have scale divisions only
every 2 cm³.

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A volumetric pipette is used to measure out a single fixed
volume of liquid very accurately. A burette is used if you want
to measure out volumes more accurately than by using a
measuring cylinder. It is also used in titrations (see Topic 11.2)
when you are not sure of the exact volume of the solution you
will be adding.

Measuring cylinders are most useful for making up fairly large


volumes of solution or in cases where accuracy is not as
important. If we want to make up a solution of a solid dissolved
in a liquid accurately we use a volumetric flask. We add the
weighed solid to the volumetric flask, then add the liquid until
it reaches the line scratched on the glass.

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Volumes of gases
Gas volumes can be measured using either a gas syringe or an
upturned measuring cylinder. A burette can also be used in this
way for greater accuracy.

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Accuracy
Accurate measurements are very close to the true value.

Three ways to improve the accuracy of results for an experiment.

• repeat your measurements in the same way each time

• use apparatus with small scale divisions

• use the apparatus carefully.

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1.6 Paper chromatography Simple chromatography
We make use of many coloured chemicals in our lives.
Ink and food colourings are just two examples.

These are often mixtures of several different dyes.


We can demonstrate this by placing a drop of ink on a piece of
filter paper.

If you then add a few drops of water to the ink, the colour
spreads out from the ink drop and separates into several
different colours.

https://youtu.be/zpf0vvkb4_8
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The colours separate if:

1. the pigments have different solubilities in the solvent,

2. the pigments have different degrees of attraction for the filter paper.

If the mixture of pigments is not soluble in water,


other solvents such as ethanol or propanone can be used.

We can get more information about the substances present in


a mixture of dyes by using a special chromatography apparatus.

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Retention factor (Rf)

3 The Rf values of four amino acids are shown below:

alanine 0.38; lysine 0.14; serine 0.27; valine 0.60

Put these amino acids in order of how far they would move up the chromatography paper.
Put the one that would move farthest first.
lysine 0.14; serine 0.27; alanine 0.38; valine 0.60
Increasing the distance of spot from the base line
Page 13 Valine 0.60 would move farthest first.
2 When setting up a chromatogram, suggest why the base line where the dyes are placed is not drawn with a pen.

Because pen ink contains a variety of compounds which would


become separated pen ink by the chromatography process.

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