Professional Documents
Culture Documents
Page 10
Page 10
measuring cylinders
Page 10
Page 10
Page 10
volumetric flasks
Page 10
There are various sizes of measuring cylinders and volumetric flasks ranging from 5 cm³ to 1 dm³.
Volumetric pipettes are made in only a few sizes, the most common being 10 cm³ and 25 cm³.
A burette is used to accurately deliver up to 50 cm³ of liquid.
Page 11
When we measure out volumes of liquids, we need to think
about the accuracy required. A burette or a volumetric pipette
is much more accurate than a measuring cylinder. The scale
divisions on a burette can be read to the nearest 0.1 cm³ but a
100 cm³ measuring cylinder might have scale divisions only
every 2 cm³.
Page 11
A volumetric pipette is used to measure out a single fixed
volume of liquid very accurately. A burette is used if you want
to measure out volumes more accurately than by using a
measuring cylinder. It is also used in titrations (see Topic 11.2)
when you are not sure of the exact volume of the solution you
will be adding.
Page 11
Volumes of gases
Gas volumes can be measured using either a gas syringe or an
upturned measuring cylinder. A burette can also be used in this
way for greater accuracy.
Page 11
Accuracy
Accurate measurements are very close to the true value.
Page 11
1.6 Paper chromatography Simple chromatography
We make use of many coloured chemicals in our lives.
Ink and food colourings are just two examples.
If you then add a few drops of water to the ink, the colour
spreads out from the ink drop and separates into several
different colours.
https://youtu.be/zpf0vvkb4_8
Page 12
The colours separate if:
2. the pigments have different degrees of attraction for the filter paper.
Page 12
Retention factor (Rf)
Put these amino acids in order of how far they would move up the chromatography paper.
Put the one that would move farthest first.
lysine 0.14; serine 0.27; alanine 0.38; valine 0.60
Increasing the distance of spot from the base line
Page 13 Valine 0.60 would move farthest first.
2 When setting up a chromatogram, suggest why the base line where the dyes are placed is not drawn with a pen.
Page 13