PLANTS AOAC ÜFFICIAL METHODS 0F

Chapter
_ 3, p. 28
___..:___ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ ANf\l.Ys1s ((1)1\¡

3.5.04 3.5.06
AOAC Official ~ethod 930,0 7
AOAC Official Method 906.01
Sucrose m Plants
Sugars (Reducing) in Plants
lnversion Methods
Munson-Walker General Method
First Actlon 1930
Flrst Actlon 1906
FinalAction
Final Actlon
A. Hydroch/oric Acid lnversion
See 906.03 (see 44.1.16).
Using aliquot of cleared solution, 931.02B (see 3
5
as in 925.05 (see 4.7.02). · .O\ ), pr°¾
3.5.05 B. /nvertase lnvers/on
AOAC Official Method 921.03
(a) For p/ants givi~g hydrolysis end point within h.- .
Sugars (Reducing) in Plants 2
aliquot of cleared solutton, 931.02B (see 3.5.01 ), into Pille!
Quisumbing-Thomas Method 400
First Action 1921 beaker and make slightly acid to methyl red with CH C~li>yrC\
3
Final Action 3 drops 1% solution of Wallerstein invertase scales L H_-Add
. et ílllXI\\¡
stand at room temperature 2 h . Add reagents as in e
A. Reagents 923
(see 44.1.15), and determine reducing power. Calculate ·1198
(a) Copper sulfate solution.-Dissolve CuSO 4-5H 2O in hot . resu\~
invert sugar. Deduct reducmg power of original sol t' as
water to make saturated solution, and filter. Determine Cu u ion al
expressed as invert sugar, and multiply difference by o.95 ' so
electrolytically and dilute solution so that 25 mL contains 525 mg
(b) For plants giving slower hydrolysis endpoint,-Plac. .
Cu (41.2 g CuSO 4-5H 2O/500 mL solution). . ) . ea11qu~
of solutton, 931.028 (see 3.5.0 1 , m small volumetric flask M
(b) Alkaline tartrate solution .-Dissolve NaOH in equal weight slightly acid to methyl red with CH3COOH. Add 3 drops I¾ · 1ake
•SO Ut10¡¡
H20 and let stand severa! days until insoluble carbonates and other ofWallerstein invertase scales and few drops toluene. Stopperflask
impurities settle out. Siphon off clear solution and determine its let stand ovemight ~r longer at ro~m temperature. Dilute to vol:
alkalinity by titration with standard acid. Dissolve 173 g highest with HP and use ahquot for reducmg power as above.
purity KNa tartrate-4H 2O (Rochelle salt) in HP in 500 mL
volumetric flask and add calculated quantity NaOH solution so that CAS-57-50-1 (sucrose)
500 mLofthis alkaline tartrate solution contains exactly 65 g NaOH.
Dilute to volume with H2O.
(e) Ferric ammonium sulfate solution.-Add 200 mL H2SO4 to 3.5.07
AOAC Official Method 930.09
ca 500 mL H 2O and cool. Dissolve 240.9 g FeNHiSO 4)r 12HP in
Ether Extract of Plants
the dilute H2 SO4 and dilute to 1 L.
Gravimetric Method
(d) Jndicator solution.-Dissolve O.IS g ophenanthroline•H2O First Action 1930
and 0.07 g FeSO4 in 10 mL H2O. Final Action

B. Determination See 920.398 (see 4.5 .01).

Measure exactly 25 mL each of CuSO4 and alkaline tartrate

solutions into 400 mL Pyrex beaker, diameter ca 9 cm. Add 50 mL 3.5.08
sugar solution containing preferably 50-150 mg sugar. Cover AOAC Official Method 930.10
beaker with watch glass and place in water bath held at 80ºC. Fiber (Crude) in Plants
After digesting exactly 30 min, filter Cu2O by suction through Digestion Method
mat of asbestos in Gooch. Wash precipitate with H2O; then with aid First Action 1930
of stirring rod transfer asbestos mat and Cu2O back into beaker in Final Action
which reduction took place. Rinse inside of crucible and lip of See 962.09 (see 4.6.01 ).
beaker with 10 mL FeNHiSO4)2 solution from pipet. Receive
rinsings in beaker containing the Cu 2O. Holding crucible over
beaker, stir contents of beaker thoroughly with stirring rod until 3.5.09
Cu 2O dissolves. Wash crucible with ca 25 mL hot water (80ºC), . AOAC Official Method 978.04
receiving washings in beaker. Stir contents ofbeaker and then raise Nitrogen (Total) (Crude Protein)
beaker to determine if any undissolved particles of Cu 2O are resting in Plants
on bottom. If so, press each one with point of stirring rod until ali Kjeldahl Methods
dissolve. Add ca 125 mL additional hot water and 1 drop indicator First Action 1978
solution. Titrate at once, with continua! stirring, with O.OS N Final Action 1978
KMnO 4. (In long titration, preferably add indicator just before end
A. Kjeldahl Method for Nitrate-Free Samples
point is reached.) Standardize KMnO4 as in 955.57 (Appendix C).
Calculate weight sugar from table, 11 th Ed. See 955.04 (see 2.4.03).
B. Kjeldahl Method for Nitrate-Containing samp/es
References: J Am. Chem. Soc. 43, 1503(1921).
JAOAC 15, 71(1932). See 970.02 (see 2.4.04).

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