Document Number : EÖ-FR.

88
Revision : 00
TED BURSA COLLEGE
Release Date : 29.09.11
Page Number : 1/1

Date: 16.09.2020
Class:10 IB
2020-2021
Name:
Subject: Calculating Magnification and Size

Calculating Magnification and Size

Scanning electron microscope image of a snout beetle
http://remf.dartmouth.edu/images/insectPart2SEM/source/20.html Image: public domain. Feb. 2012

Calculating Linear Magnification

of an Electron-Micrograph using it's scale
bar

There are three steps:

Measure the length of the scale bar - in mm.

32mm
Convert your measurement into µm.

32 x 1000 = 32000µm

Calculate the magnification.

(Magnification = measured length /the
number written. As long as you have the
SAME UNITS)
32000µm / 100 µm = 320x magnification

Magnifiation = the number of times bigger the scale bar actually

is, compared to the measurement written on it?

Scanning electron microscope image of a snout beetle

http://remf.dartmouth.edu/images/insectPart2SEM/source/20.html Image: public domain. Feb. 2012

Calculating Specimen Size

using a scale bar

There are four steps:

Measure the length of the Specimen in mm.

(show working)
83mm
Measure the length of the scale bar in mm.

32mm
Calculate how many scale bar lengths make the
specimen.
(Divide length of specimen by length of scale bar)
83mm / 32mm = 2.6
Calculate the size. Multiply the scale bar label by the last answer.
(UNITS are the same as the scale bar)

2.6 x 100 µm = 260µm

1
Calculating Magnification and Size

Scanning electron microscope image of a snout beetle

http://remf.dartmouth.edu/images/insectPart2SEM/source/20.html Image: public domain. Feb. 2012

Calculating Specimen Size

using magnification

There are three steps:

Measure the length of the Image of the

Specimen in mm. (show working)

Convert the length of the Specimen

image to µm. (x 1000)
Magnifiation = x 320

Calculate the actual size.

Divide the length of the specimen image by the
magnification (UNITS are µm)

Electron microscope image of pollen grains from oriental lily. Calculating Linear Magnification
http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html Image: public domain. Feb. 201
of an Electron-Micrograph using it's scale
bar

There are three steps:

Measure the length of the scale bar - in mm.

Convert your measurement into µm.

Calculate the magnification.

(Magnification = measured length /the
number written. As long as you have the
SAME UNITS)

0
magnification
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

0°
0 1 2 3 4 5

Magnifiation = the number of times bigger the scale

bar actually is, compared to the measurement
written on it?

2
Calculating Magnification and Size
Calculating Specimen Size
Electron microscope image of pollen grains from oriental lily. using a scale bar
http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html Image: public domain. Feb. 201

There are four steps:

Measure the length of the Specimen in mm.

(show working)

Measure the length of the scale bar in mm.

Calculate how many scale bar lengths make the

specimen.
(Divide length of specimen by length of scale bar)

Calculate the size. Multiply the scale bar label by the

last answer.
(UNITS are the same as the scale bar)
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

0°

0 1 2 3 4 5

Calculating Specimen Size

Electron microscope image of pollen grains from oriental lily.
http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html Image: public domain. Feb. 201 using magnification

There are three steps:

Measure the length of the Image of the

Specimen in mm. (show working)

Convert the length of the Specimen

image to µm. (x 1000)

Calculate the actual size.

Divide the length of the specimen image by the
magnification (UNITS are µm)

Magnification = x 2500

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

0°
0 1 2 3 4 5

3
Calculating Magnification and Size
Human leukocyte ­ showing golgi apparatus
http://remf.dartmouth.edu/images/humanBloodCellsTEM/source/3.html Image: public domain. Feb. 2012
Calculating Linear Magnification
of an Electron-Micrograph using it's scale bar

There are three steps:

Measure the length of the scale bar ‐ in mm.

Convert your measurement into nm this time.

Calculate the magnification.

(Magnification = measured length /the
number written. As long as you have the
SAME UNITS)

magnification

Magnifiation = the number of times bigger the scale

bar actually is, compared to the measurement written
on it
Calculating Specimen Size
using a scale bar

There are four steps:

Measure the length of the Specimen in mm.

(show working)

Measure the length of the scale bar in mm.

Calculate how many scale bar lengths make the

specimen. (Divide length of specimen by
length of scale bar)

Calculate the size. Multiply the scale bar label by the

last answer. (UNITS are the same as the scale bar)