MCB3020L General Microbiology Laboratory

CLASS ACTIVITY WORKSHEET

Name: Anjane Moss
Date: 06/27/2022
Section: MCB 3020L U01
Group: #8
Environment:Human

Table 1:
Test Picture Result Description
OF Medium Tube with Oil Metabolic reaction:
(anaerobic): after inoculation
testing, it is neutral
Reaction:
under aerobic
fermentative
conditions
Color: yellow/green (oxidatively) and
pH: slightly acidic fermentatively
acidic under
anaerobic conditions
Tube without Oil
(aerobic):
Enzymatic activity:
Reaction: oxidative
After incubation,
Color: green growth occurs in
pH: neutral each test tube.
Facultative bacteria
produce acid under
aerobic and
anaerobic
conditions. In this
case, the tube w/ oil
was slightly acidic,
and the tube w/o oil
was neutral.

Other:
Under acidic
conditions, the
medium will turn
yellow and blue
under alkaline
conditions. It

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appears green under

neutral conditions.
Kligler’s Triple Reaction: -- Metabolic reaction:
Iron Agar positive/negative?
positive This reaction
indicated that
Color:
glucose, lactose, and
yellow/yellow
sucrose have been
pH: acidic fermented
slant/acidic butt
Other:
Enzymatic activity:
this bacteria sample
can utilize glucose,
lactose, and or
sucrose fermenter
gas (+ or -), H2S (+
or -) which results in
a yellow/yellow
color. This means
the bacteria has an
acidic slant and
acidic butt. Since the
medium did not turn
black in the butt of
the tube, hydrogen
sulfide was not a
production

Other:

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Litmus Milk Reaction: -- Metabolic reaction:

positive/negative?
negative Fermentation of
lactose occurs under
Color: bluish/purple
acidic conditions
pH: basic causing the litmus to
Other: turn pink. In this
case no lactose was
fermented hence
why the color is
bluish which means
it is basic

Enzymatic activity:
This test results
based on the
reaction with or
proteolysis of milk
proteins in the test
tube. Because the
pH is basic, no
lactose was
fermented

Other:

Nitrate Reaction: -- Metabolic reaction:

Reduction positive/negative? w/o nitrate, there
W/o nitrate has a (+) was no color change
result, w/ nitrate has after the addition of
a (-) result both reagents
indicating nitrate
Color: w/o nitrate
can’t be determined.
there was no color
w/ nitrate, after the
change, w/ nitrate
addition of zinc the
the color changed to
bacteria is not able
red
to reduce nitrate
pH: cannot be
determined with this
test Enzymatic activity:
w/o nitrate – after

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Other: adding two reagents

the color remains
unchanged
indicating a positive
result overall
w/ nitrate – after
adding the two
reagents the color
changes to red
indicating a negative
result for the overall
test, which also
means that the
bacteria is not
capable of reducing
nitrate to nitrite

Other:
There was no color
change to both test
tubes after nitrite
was added. After
zinc was added, the
test tube w/ nitrate
turned red after a
while, meanwhile,
the test tube w/o
nitrate remained the
same
Gelatin Reaction: -- Metabolic reaction:
Hydrolysis positive/negative?
negative This tests for
liquefaction (gelatin
Color: clear
breakdown) as
pH: neutral gelatin is solid
Other: below 28 degrees
Celsius

Enzymatic activity:
After sitting in an
ice bath for ten
minutes, the gelatin

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remains solid which

indicates a negative
test result. This
means that after
growth, gelatin
breakdown does not
occur in the bacteria
sample

Other:

Urease Reaction: -- Metabolic reaction:

positive/negative?
negative This tests the ability
of the bacteria
Color: yellow
sample to produce
pH: acidic urease with agar that
Other: contains peptone
and glucose with 2%
urea

Enzymatic activity:
The yellow indicates
that no urease
enzyme is present,
but bacterial growth
causes acidic
conditions

Other:

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Starch Reaction: -- Metabolic reaction:

Hydrolysis positive/negative?
negative This tests the ability
of the bacteria
Color: yellow
sample to produce
pH: cannot be amylase enzyme
determined in this from starch
test containing agar
Other:
Enzymatic activity:
The bacteria do not
produce amylase
that is digested
within the starch
which is why there is
not a clear zone
surrounding the
colonies after the
gram’s iodine is
added

Other:

Methyl Red (48 Reaction: -- Metabolic reaction:

hours) positive/negative? this test determined
that the bacteria
positive
produced enough
Color: red fermentation under
pH: acidic acidic conditions,
therefore, this
Other: reaction is
fermentative

Enzymatic activity:
The methyl red
indicator detects a
positive result with
the color change to
a red color. This
means the bacteria
produced enough
fermentation of
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glucose to lower the

pH through the
production of stable
acids

Other:

Voges- Reaction: -- Metabolic reaction:

Proskauer (48 positive/negative? this test involves
hours) glucose
positive
fermentation
Color: red alongside with the
pH: cannot be methyl-red test
determined with this
test
Enzymatic activity:
Other:
Adding potassium
hydroxide to the
bacterial sample
resulted in a color
change to red which
indicates a positive
test result. This test
involves reaction
following glucose
fermentation.

Other:

Catalase Reaction: -- Metabolic reaction:

positive/negative? the formation of O2
gas results in
positive
bubbles and
Color: clear indicates that cells
pH: acidic growing in the
presence of oxygen.
Other: Peroxide is cleaved
into water and
oxygen gas

Enzymatic activity:

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Bubbles forming
immediately after a
portion of the colony
is exposed to
hydrogen peroxide
indicates a positive
test result. This
means that the
bacteria samples
have the catalase
enzyme present

Other:

Oxidase Reaction: -- Metabolic reaction:

positive/negative? this test allows the
bacteria with the
positive
reagent Cyt-C to
Color: dark blue oxidize the oxidase
pH: cannot be test reagent to a
determined with this color change of blue
test
Other: the color Enzymatic activity:
immediately turned
Cyt-C is present in
blue then back to its
the bacteria which
normal color
allowed it to be able
to oxidize the
oxidase test reagent
to a blue color. The
color change to blue
indicates a positive
result

Other:

NOTE: Be as descriptive as possible with each biochemical test result.