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According to Husna et. Al. (2017), Oranges is one of the most abundant and
well-known fruit which can be eaten fresh as well as to create juice. However, around
50% of the raw processed fruit waste were generated in manufacturing orange juices.
common local crop. Every year, 22,000 tons of squeezed lees from mandarin oranges
are discarded by orange juice companies in Ehime (Nikkei Business Daily, 2009).
In order to extract the biofuel in orange peels, Husna et. Al (2017) state that a
700g of orange peels were cleaned as well as manually split the pith of orange peels.
The peels were then cut into smaller pieces and were air dried in order to eliminate the
presence of moisture. The air-dried orange peels were then crush using an electric
crusher. In a jar or vessel, the petroleum ether is combined into the orange peels at a
ratio of 1:1.5 and was left to rest for a day. After 24 hours, it was filtered using a sieve.
a study by Husna
The data presented in a study by Husna et. Al. (2017) were determined by
842kg/m3. According to them, a 23.33% percentage of oil was obtained. Moreover, the
Fuel properties
The table above shows the comparison the fuel properties of orange peel oil in
comparison to Diesel and Petrol. Husna et. Al (2017) stated that, the higher the calorific
value, the higher the ability of utilizing oil as fuel. In the table above, it shows that
orange peel oil has a calorific value of 41543KJ/kg that is lower than diesel. However, it
may be utilized as fuel for other purposes. Viscosity is the most important feature of all
fuel qualities. Oil with a lower viscosity is easier to atomize as well as to pump in order
to obtain a smaller droplet for optimum mixing. The table shows that orange peel oil has
a higher kinematic viscosity than other oils. Thus, it can also be utilized as fuel for other
purposes as well.
References:
Husna, A., Ferajee, M., & Akhtar, K. (2017, December 30). [PDF] Extraction of Biofuel
https://www.semanticscholar.org/paper/Extraction-of-Biofuel-from-Orange-Peel-
and-its-Ferajee-Akhtar/3e0ba94f45fd89497b36a63a4986ba701ae88a30
Nikkei Business Daily. (2009, March). Survey Report on the Business Possibility of
At the start of the 20th century, starch was used for the first time in the United
States to produce fuel ethanol. Utilizing around 205 active plants, the majority of the
59.8 BL of bioethanol generated in the USA in 2019 was made mostly from starch.
fuel (Krajang et. Al, 2021). Moreover, cassava starch is used in a wide variety of
Thailand is cassava (Manihot esculenta Crantz), which produces over 23 million tons of
cassava root annually. Half of this crop of cassava roots is exported as cassava chips
and pellets, which are primarily utilized to make ethanol fuel. The remaining portion is
used as a raw material for the manufacture of cassava starch, which alone produces
over 1.7 million tons of solid cassava pulp waste following starch extraction.
Extraction of Bioethanol on cassava pulp and peel
yield of the samples, determine the percent sugar content of the filtrate, the percent
alcohol content of the filtrate, determine the specific gravity of the filtrate, and determine
whether or not the peaks representing ethanol bonding were present in the pretreated
sample with H2S04 acid, they used different concentrations of 2 percent, 6 percent, and
Materials
The tools used include a weighing scale, conical flasks, beakers, measuring
cylinders, spatulas, funnels, filter paper, cotton wool, and aluminum foil, as well as a
Chemicals
Sample Preparation
To remove soil and dust that had adhered to the cassava peel samples, they
were carefully washed with distilled water. The peels were exposed to the sun to dry
before being pounded into a powder using a pestle and mortar. To standardize the
particle size range of 1mm, the ground powder was then sieved through a 1mm screen.
The sample was maintained at room temperature in a container that was firmly sealed.
The organisms, Aspergillus niger and Saccharomyces cerevisiae, were taken from
cultures.
Bioethanol Production
Acid Hydrolysis
measuring 500 cm3. Distilled water, 2 percent, 6 percent, and 10 percent sulphuric acid
were then added to the appropriate conical flasks. The flasks were filled with sterile
distilled water to the 200 cm3 mark, and sterile cotton wool wrapped in aluminum foil
was inserted to prevent contamination. The samples were then heated for 2 hours at
98°C in a water bath, sterilized for 15 minutes in an autoclave, allowed to cool, and then
filtered through No. 1 Whatman filter paper. Using 10% NaOH, the pH of the filtrate
sample was brought down to 4.5. For all treatments, distilled water was used to wash
the leftover samples and create a pH balance. The four samples were dried in an oven
at 90C for 12 hours and were examined in further detail. The samples were given the
following labels:
C10 = Cassava peel samples that have been pretreated with 10% H2SO4
Fermentation Process
process (SSF). The hydrolyzed samples were placed in a conical flask, wrapped in
aluminum foil, cotton wool-lined, and autoclaved at 121C for 15 minutes. After the
autoclave, the samples were allowed to cool at ambient temperature. While the control
Saccharomyces cerevisiae were aseptically added to each flask holding the hydrolyzed
samples. The flasks were shaken and incubated for three days at room temperature (28
oC 2 oC) with cotton wool used as a stopper. To ensure a uniform distribution of the
Distillation Process
liquid was poured into a flask with a circular bottom, set on a heating mantle attached to
a distillation column, and submerged in flowing water. The distillate was collected at
78C (the normal temperature for ethanol production) in another flask that was fastened
to the other end of the distillation column. In accordance with the approach, this was
percentage of sugar content, the specific gravity and alcohol content of the filtrate, FTIR
analysis of the treated and untreated samples, and a confirmatory test for the presence
of bioethanol.
Table _: the amount of ethanol generated, the amount of sugar, the specific
The table above indicates the percentage of alcohol produced in cassava peels
as well as the quantity of ethanol, sugar content, and specific gravity as stated by
References:
Akaracharanya, A. (2010, November 15). Evaluation of the waste from cassava starch
https://annalsmicrobiology.biomedcentral.com/articles/10.1007/s13213-010-
0155-8#citeas
Caballero, B. (2003, June 28). Encyclopedia of Food Sciences and Nutrition 2nd
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/
cassava-starch
Efeovbokhan, V. E., Egwari, L., Alagbe, E. E., Adeyemi, J. T., & Taiwo, O. S. (2019).
Production of bioethanol from hybrid cassava pulp and peel using microbial and
https://bioresources.cnr.ncsu.edu/resources/production-of-bioethanol-from-
hybrid-cassava-pulp-and-peel-using-microbial-and-acid-hydrolysis/
Krajang, M. (2021, March 16). Single-step ethanol production from raw cassava starch
using a combination of raw starch hydrolysis and fermentation, scale-up from 5-L
for Biofuels and Bioproducts. BioMed Central. Retrieved July 1, 2022, from
https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-021-
01903-3
Hauwa, M., Dahiru, S., Abdulrahman, B., & Abdullahi, I.I. (2019). Ethanol Production
from Cassava ( Manihot Esculenta ) Waste Peels Using Acid Hydrolysis and
FERMENTATION PROCESS.
Corn as a Renewable Source of Biofuel
Corn grain is an efficient biofuel feedstock because of its high starch content and
ease of ethanol conversion. The corn ethanol industry benefits from a system that
allows it to produce, harvest, and store large amounts of corn. As one bushel of corn
grain produces 17 pounds of distillers’ grains and 2.8 gallons of ethanol. Farm Energy
(2019). According to the Alternative Fuels Data Center (n.d.), the starch in corn grain
produced 94% of ethanol in the US. Ethanol corn based has a positive energy balance,
which indicates that the process of generating the fuel does not consume more energy
than the fuel itself. With this, most of the gasoline in the US contains ethanol commonly
the E10 that oxygenates the fuel to reduce air pollution. Also, Ethanol is available as
E85 or flex fuel that is applied in flexible fuel vehicles while the E15 is eligible for use in
In the study of Aamri et al., (2018), it states that corn is a type of biomass
resource that can be found in farms then making it to extract biofuels. With that, the
researcher conducted an experiment where biofuels are extracted from corn and
sugarcane using the steam distillation. There is no included solvent in the process, just
the distilled water is used to dilute the required oil and disperse it with the water vapor.
The exposed material has been observed for fermentation to extract the fuel that is
The figure above simply shows the assembled experiment where the steam
distillation occurs. The methods of producing biofuel using corn are also presented.
First, the preparation for the fermentation of corn. As the dry corn is washed and
grinded into smooth consistency, it is weighed to get the needed quantity which is about
100 grams. Then, add the corn quantity to the distilled water and stir it well so it will not
get hard. By that, place the mixture into the electric heater until it is blended and when
removed from it let it cool for a while. After that, add the sulfuric acid to the solution of
the corn as well as add the prepared solution of sodium hydroxide and distilled with a
quantity of 10 ml. As the chemicals are added and to enhance the extraction of biofuels,
let it for a period of time to homogenize and interact with the solution thoroughly. With
this, prepare the yeast solution that is weighed by 8 grams. This yeast solution will be
added to the corn solution then leave it for 5 days until it ferments. Lastly, the
fermentation solution of corn will be placed in the distillation equipment where the
boiling point is at 78°C that will take 4-5 hours to extract a large quantity of biofuels.
References:
Aamri, S., Adeeb, M., & Walke, S. (2018). Production of Biofuel Using Corn and
http://www.ijtrd.com/papers/IJTRD14620.pdf
Corn for Biofuel Production. (2019, April 3). FARM ENERGY. Retrieved May 21, 2022,
from https://farm-energy.extension.org/corn-for-biofuel-production/
Ethanol Fuel Basics. (n.d.). Alternative Fuels Data Center. Retrieved May 21, 2022,
from https://afdc.energy.gov/fuels/ethanol_fuel_basics.html
Lemon as Renewable Source of Biofuel
STUDIES
In agreement with (A.T Alisarei et al., 2016), Citrus waste (CW) contains a variety
for the production of biological biofuels such as ethanol and biogas. In 2014, Iran's
horticultural product cultivation area was approximately 2.59 million hectares. In the
same year, citrus production was 4,320,041.29 t. Mazandaran Province has the highest
grade product in the processing industry, where citrus fruits generate 682,987.97 t of
waste. The findings indicated that citrus waste has a high potential for biofuel production
in Iran. CW's amount of ethanol and biogas is estimated to be 26.98 million liters and
In proportion to (B. Ashok et al., 2017), This study used a systematic approach to
determine the suitability of lemon peel oil for internal combustion engines and generator
applications. Extracted lemon peel oil is found to have a much lower viscosity, flash
point, and boiling point than conventional diesel. Various lemon peel oil blends were
prepared with traditional diesel at volumetric concentrations of 20%, 40%, 50%, and
100%, and their physical and chemical properties were evaluated for suitability for
As claimed by (B. Ashok et al., 2017), The steam distillation process extracts
lemon peel oil (LPO) from the lemon rinds. The steam vapor heats the lemon peels that
are placed on top of a grid inside the steam chamber. Fumes containing lemon essence
and steam vapor are routed to a condensing chamber to cool. A small ether is mixed
with liquid water and lemon peel oil. When this mixture is placed over a water bath, the
ether evaporates due to the heat of the water bath. This removes the volatile substance
According to D.C SIKDAR (2017), Lemon peel wastes can reduce environmental
pollution in India by reusing the peels for extraction using steam distillation. In 100 g of
Lemon peels, oil produces the best amount of citrus oil for about 1.4 ml at the optimum
temperature of 96 deg C in the time 60 min and a solid to solvent ratio of 100g/200ml.
The Gas chromatography of citrus oil states that D-limonene, Beta-Myrcene, Alpha-
Pinene, and Octanol content in the citrus oil is 94.13%, 3.79%, 1.24%, and 0.8%.
Bangalore, India. It will be the compound for the steam distillation process. It is
manually Cleaned and pith the lemon peels that are pre-heated at a temperature of 45
deg C and kept for two hours. A distillation flask, basket heater, horizontal condenser,
and conical flask perform the distillation unit. The distillation flask has 100 g of pre-
treated lemon peels, then put a 200mL of water. The basket heater is temperature-
In the initial stage, the temperature is 88 deg C for 60 min then the distillate is
contained in the conical flask. The distillate has two layers, and with the help of a
separating funnel, the less thick upper layer gets the citrus oil kept in a glass bottle.
time and the solid to solvent ratio start to vary the temperature at an interval of 2 deg C
Based on the result, as the temperature increases, oil yield increases at the
state nothing and are still the same due to having a high temperature that lemon peels
keeping the temperature and solid to solvent ratio constant starts to vary the distillation
time with an interval of 15 min from 15 min to 75 min giving the best distillation time.
In the given result, as the distillation time increases, the oil yield increases in the
maximum time of 60 min duration. But as the distillation time increases to 75 min, the oil
For the last phase, keeping the distillation time and temperature constant to vary
the solid to solvent ratio from 100g/160 mL to 100 g/ 240 mL gives the best condition for
The result shows that as the distillation content solid to solvent ratio increases,
the oil yield increases and reaches only 100g/200mL solid to solvent ratio. As it
increases, the oil yield decreases due to the bubbling that occurs, affected by the high
source of fuel by using the direct injection diesel engine without using any
transesterification process due to the low viscosity and low boiling point in nature. The
100% lemon peel oil fueled the diesel engine that gives a 12% higher brake thermal
fuel in a full load condition. Significant reductions have been observed in BSCO, BSH
Can smoke emissions with 100% lemon peel oil in diesel engine without any
modifications.
distillation process from the lemon rinds. The water will be in the main steam
distillation chamber. The water converted to steam absorbs the heat through the
bottom walls, lemon peels are on the top of the grid inside the steam chamber
heated by the steam vapor, and lemon peel steam goes to condensing for
cooling purposes. The mixture of liquid water and lemon peel oil collected in the
tank will separate due to their density. Along with the certain impurities joined in
the lemon peel will be drained into the collecting tank and mixed with a small
quantity of ether. The mixture will be over a water bath, then the heat of the water
bath makes the ether evaporate, leaving the pure lemon peel oil left behind to
remove the volatile substance present in the oil. Finally, the solid particles are
states the different physical and chemical properties of lemon peel oil and diesel fuels.
The boiling point is 176 deg C and the chemical used is Ethanol.
The result shows the comparative properties of tested fuels that various blends of
lemon peel oil-diesel have been prepared by increasing the concentration of LPO in the
volumetric proportion of 20%, 40%, 50% and 100%. It indicates that the LP020
preparation is blending 20% lemon essential oil with 80% diesel. The LPO20 biodiesel
was used as fuel in a compression-ignition engine and found that LPO20 performed
LITERATURE
carbohydrates and fermentable sugars are converted into biogas and ethanol.
Compared to gasoline or petroleum fuel, biofuels can reduce carbon dioxide emissions
fruit waste account for 20–50% of household waste, while citrus peel waste (CPW) is a
significant residue under the specific category. Global citrus production (orange, lemon,
lime, know, sweet orange, and so on) exceeds 121 106 t per year, while the industrial
during fruit processing consists primarily of peels and pressed pulp (seeds and segment
As per Kesterson and Braddock (1975), a suitable method can extract 5–11.5 kg
of lemon peel oil and 3–5 kg of orange peel oil from one Metric Ton (MT) peel. In this
study, the standard cold-pressed distillation method extracts lemon peel oil from its
rinds. The various physical and chemical properties of lemon peel oil have been
engines.
Biomall (2017) indicates that scientists at the VIT University at Vellore in India
found a way to extract biofuel from lemon fruits. K Nanthagopal and the other
researcher extract the lemon essential oil from lemon rinds using the steam distillation
The flash and fire point may have nearly similarities, but when it comes to handling and
transportation, a lemon essential oil is easy compared to diesel fuel because of its lack
of viscosity and fatty acid compositions easily atomized during the combustion process.
It is concluded that 50% lemon peel oil could be readily used in diesel engine and
that having a low viscous and low boiling point fuel like lemon essential oil blended with
diesel accelerates the air-fuel mixing process, enhancing the evaporation process in
REFERENCES (LITERATURE):
Biomall. (2017, November 20). Lemon essential oil as a cleaner, partial biofuel
substitute for petroleum diesel. Retrieved May 19, 2022, from Biomall Blog
website: https://blog.biomall.in/lemon-essential-oil-as-a-cleaner-partial-
biofuel-substitute-for-petroleum-diesel/
Mahato, N., Sharma, K., Sinha, M., Dhyani, A., Pathak, B., Jang, H., Park, S.,
q=https://www.mdpi.com/2227-9717/9/2/220/
pdf&sa=D&source=docs&ust=1653132007734297&usg=AOvVaw1WwYjR
wSAxp0WjRs4a51Yy
Patsalou, M., Samanides, C. G., Protopapa, E., Stavrinou, S., Vyrides, L., &
Koutinas, M. (2019, July 4). A Citrus Peel Waste Biorefinery for Ethanol
https://www.google.com/url?q=https://www.mdpi.com/1420-
3049/24/13/2451/
pdf&sa=D&source=docs&ust=1653130202496920&usg=AOvVaw37bCCV
7MxENhmXqO_Bqtb7
https://ift.onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1975.tb02236.x
Alisaraei, A. T., Hosseini, S. H., Ghobadian, B., & Motevali, A. (2016, October).
https://www.researchgate.net/publication/309154314_Biofuel_production_f
rom_citrus_wastes_A_feasibility_study_in_Iran
paper=extraction-of-citrus-oil-from-lemon-citrus-limon-peels-by-steam-
distillation-and-its-characterizations.pdf
Ashok, B., Raj, R. T. K., Nanthagopal, K., Krishnan, R., & Subbarao, R. (2017,
from
https://www.academia.edu/31622356/Lemon_peel_oil_A_novel_renewabl
e_alternative_energy_source_for_diesel_engine
Ashok, B. & Kasianantham, Nanthagopal & Balusamy, Saravanan & Muniappan,
SenthilKumar & Dandu, Madhu & Subhash, Randive & Pravin, Nashte &
diesel engine using novel emulsified lemon peel oil biofuel. Environmental
According to Darko, K. et al. (2009), to reduce the costs associated with the
production of biofuel and the cost of electricity, it is highly recommended to use soybean
straw such as Neoplanta, Ika, Podravka, Tisa, and Vita as an alternative to more
extensively in North America, Asia, and South America. The United States began
expanding its oilseed crop in the 1940s and now accounts for 32% of global soybean
production, while Brazil accounts for 28%. Soybeans, which originated in Southeast
Asia, were first domesticated in China in the 11th century BC. Soybeans have spread
throughout the Corn Belt since their introduction in 1765 and reached their peak
acreage in the 1920s, when they were primarily used for forage (Gibson & Benson,
more energy than is needed to grow the crops and convert them into biofuels. It
produces 93% more energy than it consumes. In accordance with Boumahdi (2019),
soy oil extraction is required for the production of soybean oil, wherein the most
common form of this product is refined, bleached, and deodorized (RBD). Cleaning and
degumming soybean oil are performed during the refining process to remove any
remaining impurities and lecithin. The following step is bleaching to remove any
remaining pigments and residues. And lastly, the oil is deodorized by going through a
process called steam distillation, which gets rid of any volatile components that are still
left.
References
Boumahdi, F. (2019, April 30). RBD Soybean Oil Applications. Eco Link.
https://ecolink.com/info/rbd-soybean-oil-applications/
Darko, K., Branko, S., Vlado, G., Voća, N., Rozman, V., & Luka, Š. (2009, December).
https://www.researchgate.net/profile/Neven-Voca/publication/41120409_Soybea
n_Biomass_as_a_Renewable_Energy_Resource/links/
5732f9df08ae9f741b25504e/Soybean-Biomass-as-a-Renewable-Energy-
Resource.pdf
Gibson, L. & Benson, G. (2005). Origin, History, and Uses of Soybeans (Glycine max).
University Of Minnesota. (2006, July 11). Soybean Biodiesel has higher net energy
https://news.mongabay.com/2006/07/soybean-biodiesel-has-higher-net-energy-
benefit-than-corn-ethanol/amp/