Urine Sediment Guide

All images from the SediVue Dx ®

Urine Sediment Analyzer

Reference bar = 20 microns

Blood cells

Figure 1. Red blood cells Figure 2. Crenated red blood cells Figure 3. White blood cells Figure 4. White blood cells

Epithelial cells

Figure 5. Squamous epithelial cells Figure 6. Squamous epithelial cells Figure 7. Numerous transitional (nonsquamous) Figure 8. Numerous transitional (nonsquamous)
epithelial cells with RBCs and WBCs epithelial cells (Possible transitional cell
carcinoma. Confirm with dry-slide cytology.)

Bacteria

Figure 9. Rods with white blood cells Figure 10. Rods with white and red blood cells Figure 11. Cocci with white blood cells Figure 12. Cocci in chains

Casts

Figure 13. Left and right, hyaline cast Figure 14. Cellular (nonhyaline) cast Figure 15. Numerous granular (nonhyaline) casts Figure 16. Waxy (nonhyaline) cast

Crystals

Figure 17. Large struvite crystals Figure 18. Numerous small struvite crystals Figure 19. Large calcium oxalate dihydrate Figure 20. Numerous calcium oxalatedihydrate
crystals crystals

Figure 22. Calcium oxalate monohydrate Figure 23. Ammonium biurate (thorn apple) Figure 24. Bilirubin crystal with WBCs
Figure 21. Calcium oxalate monohydrate crystals; left, dumbbells; right, hemp seed crystals
(picket fence) crystals

Figure 26. Cystine crystals with red blood Figure 27. Uric acid crystals Figure 28. Likely drug-related crystals
Figure 25. Cholesterol crystals cells

Miscellaneous

Figure 29. Lipids Figure 30. Amorphous crystalline debris Figure 31. Hyphae Figure 32. Sperm with white blood cells

Figure 33. Left, Pearsonema spp. Figure 34. Left, glove powder; right, pollen Figure 35. Fiber Figure 36. Dust mite
(Capillaria spp.) ova; right, macrocanidia
Conventional microscopy
All images, unless otherwise indicated, are representative of a high power (40x objective) field of view.
Blood cells
Cells

Figure 1. Erythrocytes and one Figure 2. Erythrocytes and two Figure 3. Numerous leukocytes andfew
squamous epithelial cell leukocytes (black arrows) rod-shaped bacteria
Epithelial cells

Figure 4. Squamous epithelial cells Figure 5. Epithelial cells (black arrows), Figure 6. Transitional epithelialcells
RBC (red arrows) and WBC (blue arrows)

Figure 9. Transitional cell carcinoma,

Figure 7. Left, Transitional cell Figure 8. Transitional cell carcinoma air-dried and Diff-Quik* stained
carcinoma; right, NMB wet prep (NMB wet prep on right)

Bacteria

Figure 10. Many rod-shaped Figure 11. Many leukocytes and large Figure 12. Numerous bacteria and
bacteria,100× objective field of view rod-shaped bacteria (black arrows) leukocytes
Casts

Figure 13. Hyaline cast (borders Figure 14 Left, granular cast; right, Figure 15. Waxy cast
outlined) mixed waxy and granular cast
Crystals

Figure 16. Struvite Figure 17. Amorphous (NMB wet Figure 18. Bilirubin
prep on right)

Figure 19. Ammonium biurate Figure 20. Left, calcium oxalate Figure 21. Drug (Tribrissen*) crystals,
monohydrate; right, calcium oxalate 10× objective field of view
dihydrate
Miscellaneous

Figure 22. Left, fat droplets (red Figure 23. Pearsonema plica Figure 24. Contaminant fragmented
arrows, RBC); right, sperm fiber
Conventional images and information provided by Dennis B. DeNicola, DVM, PhD, DACVP; Rick L. Cowell, DVM, MS, MRCVS, DACVP; and Graham Bilbrough, MA, VetMB, CertVA, MRCVS.
 How to perform a dry prep/line smear
Melakukan prep kering atau apusan garis adalah cara yang sangat hemat biaya untuk
mengkonfirmasi ada atau tidaknya bakteri, membedakan antara kokus dan batang
pendek, dan untuk mengkarakterisasi berbagai elemen seluler dalam sampel urin.
1.Label slide Anda dengan tepat.
2.Isi tabung sentrifus dengan urin segar yang tercampur dengan baik yang diambil
dari bagian bawah tabung sampel.
3.Centrifuge sampel (dan tabung keseimbangan) pada pengaturan Urine (atau 400 g).
Catatan: Jika centrifuge Anda tidak memiliki Urine
pengaturan, lihat manual operatornya untuk pengaturan dan waktu sentrifugasi.
4.Setelah sentrifugasi, pelet terkonsentrasi dari elemen yang terbentuk harus terlihat
di bagian bawah tabung.
Aspirasi supernatan secara perlahan hingga mencapai pelet, sisakan urin dalam
jumlah yang sangat sedikit untuk mengsuspensikan kembali pelet.
Catatan: Jika sampel sangat hiposelular, mungkin sangat sulit untuk melihat pelet.
5. Jentik perlahan bagian bawah tabung beberapa kali dengan jari Anda untuk
menangguhkan kembali dengan lembut

6. Dengan menggunakan pipet baru, tuangkan setetes sampel pada kaca objek, mirip
dengan pembuatan film darah.
7.Tempatkan kaca objek pengoles kaca yang bersih pada kaca objek berlabel, kira-
kira 30°–40°, di depan tetesan urin.
8. Kembalikan slide spreader ke dalam drop yang memungkinkan material menyebar
di sepanjang tepi slide spreader.
9. Pindahkan slide penyebar ke ujung slide spesimen, jaga agar keduanya tetap
bersentuhan.
10. Di tengah slide, hentikan penyebaran sampel urin secara tiba-tiba dan angkat
slide spreader lurus ke atas hingga membentuk garis bahan.
11. Keringkan secara menyeluruh lalu nodai slide menggunakan pewarnaan
hematologi/sitologi rutin Anda (mis., Diff-Quik*).
12. Tinjau secara mikroskopis.