Name: B-Aliifah-205070100111051

Exercise 1: Cell Transport Mechanisms and Permeability: Activity 5: Simulating Active Transport Lab Report

Pre-lab Quiz Results

You scored 50% by answering 2 out of 4 questions correctly.

1. The movement of sodium and potassium maintained by the Na+ -K+ pump
Your answer : a. is against a concentration gradient.
Correct answer: e. requires energy and is against a concentration gradient.

2. The sodium-potassium pump is classified as a(n)

You correctly answered: a. antiporter.

3. The sodium-potassium pump moves _____ sodium ions and _____ potassium ions simultaneously.
Your answer : c. 3, 3
Correct answer: b. 3, 2

4. Solutes that require active transport for movement might be too large to pass or might be
You correctly answered: a. lipid insoluble.

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Experiment Results
Predict Question:
Predict Question 1: What do you think will result from these experimental conditions?
Your answer : a. Na+ will be maximally transported.

Predict Question 2: Do you think the addition of glucose carriers will affect the transport of sodium or potassium?
Your answer : d. No, it will not affect the transport of either ion.

Stop & Think Questions:

Why did the sodium transport stop before the transport was completed?
You correctly answered: c. The ATP was depleted.

Why was the equilibrium for the solutes reached earlier?

You correctly answered: c. There were more pumps for transport.

Experiment Data:

Run Number Solute ATP Start Conc. L Start Conc. Pumps Carriers Rate
R
1 Na+ Cl- 1 9.00 0.00 500 --- 0.0188
1 K+ Cl- 1 0.00 6.00 500 --- 0.0125
1 Glucose --- 0.00 0.00 --- 0 0.0000
2 Na+ Cl- 3 9.00 0.00 500 --- 0.0025
2 K+ Cl- 3 0.00 6.00 500 --- 0.0017
2 Glucose --- 0.00 0.00 --- 0 0.0000
3 Na+ Cl- 3 9.00 0.00 500 --- 0.0000
3 K+ Cl- 3 0.00 0.00 500 --- 0.0000
3 Glucose --- 0.00 0.00 --- 0 0.0000
4 Na+ Cl- 3 9.00 0.00 800 --- 0.0042
4 K+ Cl- 3 0.00 6.00 800 --- 0.0028
4 Glucose --- 0.00 0.00 --- 0 0.0000
5 Na+ Cl- 3 9.00 0.00 800 --- 0.0042
5 K+ Cl- 3 0.00 6.00 800 --- 0.0028
5 Glucose --- 0.00 10.00 --- 400 0.0028#

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Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. What happened when you increased the amount of ATP dispensed with the same concentration of sodium and
potassium on either side of the membrane?
You correctly answered: b. More ions were transported.

2. At what concentration of ATP were the sodium and potassium maximally transported?
You correctly answered: d. 3 mM ATP

3. What was the effect of adding more Na+ -K+ pumps to the simulated cell?
You correctly answered: a. Transport of the ions was faster.

4. Describe the effect of adding glucose carriers to the sodium and potassium transport.
You correctly answered: c. There was no change in the transport rate because glucose is transported independently.

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Review Sheet Results
1. Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell,
instead of other concentration ratios.
Your answer:
Agar banyak sodium (Na) yang keluar dan potassium (K) yang masuk pada transport membran seimbang, maka rasionya
harus 3:2

2. Explain why there was no sodium transport even though ATP was present. How well did the results compare with your
prediction?
Your answer:
Saya pada awalnya berasumsi jika sodium nantinya akan ditranspor secara maksimal meskipun terdapat ATP. Namun,
ternyata sodium tidak ditranspor karena air berpindah masuk ke membran dengan cara osmosis.

3. Explain why the addition of glucose carriers had no effect on sodium or potassium transport. How well did the results
compare with your prediction?
Your answer:
Glukosa tidak mempengaruhi transport sodium dan potassium karena ia melakukan difusi terfasilitasi dengan menggunakan
protein membran.

4. Do you think glucose is being actively transported or transported by facilitated diffusion in this experiment? Explain your
answer.
Your answer:
Glukosa mengalami difusi terfasilitasi yang menggunakan protein membran. Karena ukuran molekulnya yang besar,
terdapat perbedaan konsentrasi dan bahan berpindah sesuai gradien konsentrasi agar keseimbangan tercapai.

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