Collection of urine for Collect 10-mL of

urine around mid-

URINE the diagnosis of day from 10 AM to
Schistosoma infection 2 PM

Avoid the first jet of urine.

Collect the central part of the
urine or also called terminal
urine

URINE
EXAMINATION

Shake the urine

Sedimentation specimen well and
Allow the
method pour into a conical urine to
urine flask sediment for 1
hour

Examine the
Withdraw the
deposit of the
supernatant then
centrifuge sample
transfer it into a
for the sample of Centrifuge at
2000 g for 2 centrifuge
ova using 10×
minutes
objective

Place a polycarbonate
Syringe filtration or nylon filter (pore
method size 12-20µm) in the
filter holder

Draw 10mL of the

Carefully unscrew the filter Expel the urine from urine into the syringe
holder, draw air into the the syringe into the and attach the filter
syringe, reacttach the syringe filter holder over a holder to the bottom
Keep the urine
to the holder, and expel the air bucket or sink at unit level of the syringe

Unscrew the filter holder, remove Examine the whole

the filter with the forceps, and filter under the
place it on a microscope Add 1 drop of microscope at 40x
Lugol’s iodine
and wait for 15
seconds for the
stain to penetrate
Schistosome eggs are
stain in orange when
viewed under
microscope