_______________________________________________________________________________________________________________________

Patient's Name : Haresh Ghelanee Colle. Date/Time : 17/01/2022 10:54

Age/Sex : 24 Years / Male Sample Location : FACILITY CENTRE
Referred by : Self Ref.No. : COVID-61628-22
Reg. Date/Time : 17/01/2022 10:55 *7720*
_______________________________________________________________________________________________________________________

NOVEL CORONA VIRUS / 2019 nCoV / SARS-CoV-2 ICMR APPROVED

Qualitative RT PCR by ROTOR GENE Q , GERMANY
_________________________________________________________________________________________________________________________

SAMPLE TYPE 1. Nasopharyngeal swab 2. Oropharyngeal swab

METHOD REAL TIME PCR

TARGET RESULT CT VALUES INTENDED USE

N gene Detected 16 Confirmation

( Nucleocapsid gene )

ORF 1ab gene Detected 16 Confirmation

RESULT N , ORF 1ab gene detected

INTERPRETATION 2019-nCoV Detected

ICMR NO

PASSPORT NO

CT VALUE > 33 : LOW VIRAL LOAD , CT VALUE BETWEEN 23 TO 33 : MODERATE VIRAL LOAD ,
CT VALUE < 23 : HIGH VIRAL LOAD CT VALUE > 37 : NEGATIVE
_________________________________________________________________________________________________________________________
Note : Viral load differentiation is for better understanding of diseased condition and patient treatment . Low viral load

must be correlated with clinical and radiological correlation for better interpretation .

DISCLAIMER

Test results are highly dependent on sample type and its sensitivity , sampling techniques , biological variation
and patients clinical condition.
Presence of PCR inhibitors ( can not be traced by technologist ) or viral load below LOD for the ICMR approved kits
used and or mutant genotypes may result in false negative report. False positive results may be obtained due to
background RNA enviornmental contamination in lab before it is identified and corrective and preventive actions
are taken . Results must be correlate clinically and other relavant history. This test is not accredited by FDA , NABL
or CAP. Each batch of assays is run along with positive and negative control and kits approved by ICMR. There are
all chances of result variations between various perforing labs and institute due to preanalytical , analytical and
postanalytical variables. like sample collection methods , expertize , sample transport and preservation , difference
in kit sensitivity , interinstrumental variations and rest ,

Page 1 of 2
_______________________________________________________________________________________________________________________

Patient's Name : Haresh Ghelanee Colle. Date/Time : 17/01/2022 10:54

Age/Sex : 24 Years / Male Sample Location : FACILITY CENTRE
Referred by : Self Ref.No. : COVID-61628-22
Reg. Date/Time : 17/01/2022 10:55 *7720*
_______________________________________________________________________________________________________________________

Fact File

Coronavirus is a single-stranded positive-sense RNA virus with an envelope of about 80 to 1 20 nm in diameter. lts genetic
material is the largest of all RNA viruses and is an important pathogen of many domestic animals, pets, and human
diseases. It can cause a variety of acute and chronic diseases. Common signs of a person infected with a coronavirus
include respiratory symptoms, fever, cough, shortness of breath, and dyspnea. In more severe cases, infection can cause
pneumonia, severe acute respiratory syndrome, kidney failure, and even death. The 2019 new coronavirus. or
'2019--nCoV". was discovered because of Wuhan Viral Pneumonia cases in 2019, and was named by the World Health
Organization on January 12, 2020, confirming that it can cause colds and the Middle East Respiratory Syndrome (MERS)
and more serious diseases such as acute respiratory syndrome (SARS).

A novel coronavirus (nCoV) identified in China as COVID 19 or SARSCoV-2 is a new strain that has not been previously
identified in humans. Since then COVID-19 has spread to many more countries - cases have been reported in all over the
world. Coronavtuses are zoonotic and are transmitted between animals and people. At present, there is no cure for
COVID-19. Standard recommendations to prevent infection spread include regular hand washing, covering mouth and
nose when coughing and sneezing, thoroughly cooking meat and eggs. Avoid close contact with anyone showing
symptoms of respiratory illness such as coughing and sneezing. Early detection is very crucial for clinical management
and disease spread. Real time PCR technology is recommended for early detection by CDC.
SENSITIVITY : 100 RNA copies / reaction
SPECIFICITY : Highly specific as no cross reactivity with most of the coronaviruses , influenza , parainfluenza viruses ,
rhinoviruses , Adenoviruses Legionella pneumophilia serotypes and Mycoplasma pneumoniae

If a sample with a variant that has the 69-70del mutation is tested using the TaqPath COVID-19 assay, it will result in an S
gene "dropout." Because of this, the test may signal the presence of the 69-70del mutation and, potentially, the B. 1.1. 7
lineage (variant 501Y. V1). Recently, multiple novel strains of SARS-CoV-2 have been found to share the same deletion of
amino acids H69 and V70 in the virus S gene. This includes strain B.1.1.7 / SARS-CoV-2 VUI 202012/01, which has been
found to be more infectious than other strains of SARS-CoV-2 The new SARS-CoV-2 lineage-B.1.1.7-poses a challenge to
these efforts. According to the European Centre for Disease Control and Prevention (eCDC), "while it is known and
expected that viruses constantly change through mutation leading to the emergence of new variants, preliminary
analysis in the UK suggests that this variant is significantly more transmissible than previously circulating variants" .

Verified By : ALPES dr. kaushik patel

Verification Time : 17/01/2022 14:26:00 Print Time : 17/01/2022 18:04:00 md(path.&bact.)
-----------------------end of report-----------------------

Page 2 of 2