Name: ANTONIEGA, KEN PALERMO Section: STEM 12 - ANTHRACITE

GENERAL BIOLOGY II
Learning Activity Sheet 1: GENETIC ENGINEERING

I. Enumerate and discuss the process involved in Genetic Engineering.

STEP 1: Identity section of DNA that contains required gene from source chromosome.
STEP 2: extract required gene,
STEP 3: insert the required gene into vector/bacterial plasmid,
STEP 4: insert plasmid into the host cell,
STEP 5: grow transformed cells to produce a GM organism.

II. Study the figures below. Choose the correct process of genetic engineering inside the box and
write your answers on the box provided for each step.

* Multiplying the plasmid in bacteria * identification of the gene interest

* Removal of plasmid from bacterial cell * required gene is inserted into bacterial plasmid
* Identified gene is extracted from the chromosome

Identification of the gene Identified gene is extracted

interest from the chromosome

Required gene is inserted

into bacterial plasmid

Multiplying the plasmid in

bacteria

Removal of plasmid from bacterial

cell
III. Give one example made by Genetic Engineering. Cite your source.
Glow-in-the-Dark Cat
The Glow-In-The-Dark Kitty
A fluorescent green cat could help in the fight against AIDS
Author: Sarah Zielinski

A variant of the green fluorescent protein that lights up the crystal jelly, a type of jellyfish found off the
West Coast of the United States, is responsible for the cat's glow. Scientists discovered years ago that the
GFP gene serves as an excellent identifier for inserting a new gene into an organism. They could easily
see if they were successful by adding a version of GFP along with their gene of choice, as the creature
would glow. Many glowing animals have been created since the technology was initially invented,
including pigs, mice, dogs, and even pet store fish.
The Mayo Clinic researchers put a version of the GFP gene, along with a gene from the rhesus macaque
that prevents the feline immunodeficiency virus (FIV)—the virus that causes feline AIDS—into the
unfertilized eggs of a cat in this latest study, which was published in Nature Methods. After the eggs were
fertilized, the kittens became green, indicating that they contained the anti-FIV gene as well. Even better,
the anti-FIV gene was passed down to succeeding generations of cats.
However, the ultimate goal of this line of research is to figure out how to make humans immune to HIV,
the virus that causes AIDS. "We want to see if we can protect the domestic cat from its AIDS virus, if we
can protect any animal from its own AIDS virus, including ours," Poeschla told LiveScience.

(Mayo Clinic reports that a luminous cat could aid in the fight against AIDS.)

Reference:
https://www.smithsonianmag.com/science-nature/the-glow-in-the-dark-kitty-77372763/