ST.

ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com

Name: Score:
Course, Year & Section: Date performed:

Activity 4: Test for Lipids

Objectives:
At the end of the experiment student should be able to:
 Examine physical and chemical properties of lipids.
 Compare the properties of soap with detergent.

Procedure:
A. Spot Test
1. Using the oils available in your home identify three, that you could use in this
experiment it could be an essential oil, cooking oil or coconut oil.
2. Place a drop of that sample on a piece of bond paper.
3. Allow it to dry and note any transparency produced.

Test Sample Observation

B. Surface Tension
1. Using a clean and dry dropper and record the number of drops that will be
formed from 1ml of pure distilled water in a glass.
2. Now rinse the dropper with a soap solution.
3. Record the number of drops from 1ml of the soap solution without rinsing the
dropper fill it with pure distilled water and again note the number of drops.
Soap Solution Number of Drops
 C. Dietary Fat Chart

D. Nutritional Label Analysis

1. Find the nutrition Facts label on one food item wrapper, then answer the
questions on your nutritional label analysis hand out. When you are finished,
answer the same questions for the remaining two food items.
2. Next, choose four different fat-containing food items and determine a serving size
for each so that the total amount of food you select contains one day’s worth of
recommended dietary fat for teen.
Note:
 Total fat calories for one day for girls average about 650 calories (more for
athletes). Ten percent or less of total daily calories may come from saturated
fats.
 Total fat calories for one day for boys average about 700 calories (more for
athletes). Ten percent or less of total daily calories may come from saturated
fats.
 Grams of saturated fat should be no more than 24 for girls and no more than 27
for boys.

Food Item 1 Food Item 2 Food Item 3

How many calories
per serving from fat?

What is the percent

daily value from fat?

How much saturated

fat does the item
contain?
How much
monounsaturated
(heart healthy) fat
does the item
contain?

How much
polyunsaturated
(heart-healthy) fat
does the item
contain?

How much trans fat

does the item
contain?

Does the food item

have high sodium,
which may also play a
role in heart disease?

Type of Fat Description Foods Potential Effect Potential Effect

containing the on HDL on LDL
fat
Saturated from animal butter, meat, Raises Raises
based food whole milk,
items and some coconut oil
plants

Monounsaturated This type of fat Olive oil, canola Raises Reduce

is found in a oil, peanut oil,
variety of foods safflower oil,
and oils.  sesame oil
Polyunsaturated

Trans Fat

Learning Questions:
1. What important roles do lipids perform in living organisms?

2. What is the difference between good and bad cholesterol?