ST.

ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com

Name: Score:

Course, Year & Section: Date performed:

Activity 4: Test for Lipids

Objectives:

At the end of the experiment student should be able to:

● Examine physical and chemical properties of lipids.

● Compare the properties of soap with detergent.

Procedure:

A. Spot Test
1. Using the oils available in your home identify three, that you could use in this experiment it could
be an essential oil, cooking oil or coconut oil.
2. Place a drop of that sample on a piece of bond paper.
3. Allow it to dry and note any transparency produced.

Test Sample Observation

B. Surface Tension
1. Using a clean and dry dropper and record the number of drops that will be formed from 1ml of
pure distilled water in a glass.

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 ST. ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com

2. Now rinse the dropper with a soap solution.

3. Record the number of drops from 1ml of the soap solution without rinsing the dropper fill it with
pure distilled water and again note the number of drops.

Soap Solution Number of Drops

C. Nutritional Label Analysis

1. Find the nutrition Facts label on one food item wrapper, then answer the questions on your
nutritional label analysis hand out. When you are finished, answer the same questions for the
remaining two food items.
2. Next, choose four different fat-containing food items and determine a serving size for each so that
the total amount of food you select contains one day’s worth of recommended dietary fat for teen.

Note:

● Total fat calories for one day for girls average about 650 calories (more for athletes). Ten percent
or less of total daily calories may come from saturated fats.
● Total fat calories for one day for boys average about 700 calories (more for athletes). Ten
percent or less of total daily calories may come from saturated fats.
● Grams of saturated fat should be no more than 24 for girls and no more than 27 for boys.

Food Item 1 Food Item 2 Food Item 3

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 ST. ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com

How many calories per

serving from fat?

What is the percent daily

value from fat?

How much saturated fat

does the item contain?

How much
monounsaturated (heart
healthy) fat does the item
contain?

How much
polyunsaturated (heart-
healthy) fat does the item
contain?

How much trans fat does

the item contain?

Does the food item have

high sodium, which may
also play a role in heart
disease?

D. Dietary Fat Chart

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 ST. ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com

Type of Fat Description Foods containing Potential Effect on Potential Effect on

the fat HDL LDL

Saturated from animal based butter, meat, whole Raises Raises

food items and some milk, coconut oil
plants

Monounsaturated This type of fat is Olive oil, canola oil, Raises Reduce
found in a variety of peanut oil, safflower
foods and oils.  oil, sesame oil

Polyunsaturated

Trans Fat

Learning Questions:

1. What important roles do lipids perform in living organisms?

2. What is the difference between good and bad cholesterol?

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 ST. ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com

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