GARCIA HANNA CASANDRA L.

BSMLS-3-Y1-3
PERFORMANCE TASK A. STAN BIO GLUCOSE OXIDASE METHOD
Solve the following:
Data and Results: Demographics
Au= 0.250
As= 0.230
Computations: put your final in a box.
Glucose(mg/dl) = Au/As x 100
0.250/0.230 x 100
1.086956522 x 100

= 108.69 0r 108.70

Interpretations: Above the reference range

CRITICAL THINKING:
Do not copy your answer from the internet. no cheating. No plagiarism.
1. Compare the method of Stan bio with the Profame in determining the glucose in the serum.
 STANBIO
β-D- Glucose+H20+02 → GODase →H202 +D-gluconic acid
H202 + 4-aminoantipyrine+phenol → POD → Quinone complex + H20
 PROFAME
Glucose condenses with O-toluidine in glacia acetic acid when heated at 100 °C forming N- glycosylamine
Glucose + 0-toluidine →N-glycosylamine

2. Why is it important that the laboratory to establish their own reference range?
 STANBIO- To diagnose and treat hyperglycemia and hypoglycemia, precise glucose assessment is
crucial. Knowing the range is crucial since hyperglycemia can happen due to diabetes mellitus, in
patients receiving intravenous glucose infusions, or under extremely stressful conditions. A
carbohydrate metabolism inborn defect, fasting, an insulinoma, or insulin injection can all cause
hypoglycemia.
 PROFAME- Several hormones, primarily insulin and glucagon, keep the blood glucose levels at
5mmol/L or less. Neurological and other problems could develop if the blood glucose level drops
below this mark (hypoglycemia). On the other hand, hyperglycemia sets in when blood sugar levels
rise to 10 mmol/L above usual. This is a sign of diabetes mellitus.

3. Aside from FBS, what are the other test to measure sugar in the serum?
 Other procedures include the oral glucose tolerance test (OGTT) and intravenous glucose tolerance
test (IVGTT), in which a predetermined amount of glucose is given orally or intravenously,
 respectively, and subsequent blood sugar tests are taken to assess how well the body handles the
substance.

4. what are the specimen considerations for glucose measurement.

 SERUM- Within 30 minutes after collection, remove from the clot to stop glycolysis.
 PLASMA- Any of the typical anticoagulants may be used if plasma is quickly separated from cells
after centrifugation, however fluoride-containing anticoagulants are advised.

PERFORMANCE TASK B: GLUCOSE DETERMINATION USING PROFAME

Answer the following study questions about glucose
Guidelines
Study Questions:
1. Name the three classes of carbohydrates according to number of sugar units.
 Monosaccharides
 Oligosaccharides
 Polysaccharides

2. What is the main role of Carbohydrates in the body?

 The main source of energy for the body and the preferred energy source for the brain are
Carbohydrates. Your kidneys, central nervous system, heart, brain, and muscles can all benefit from
it. For instance, fiber is a carbohydrate that supports digestion, makes you feel satisfied, and lowers
blood cholesterol levels

3. Explain hyperglycemia and hypoglycemia.

 When blood sugar levels are excessively high, hyperglycemia develops. Those with untreated
diabetes can have hyperglycemia. When blood sugar levels are too low, hypoglycemia develops.
This is typically an adverse effect of blood sugar-lowering drug therapy.

4. Explain the difference between NIDDM and IDDM.

 In those with NIDDM, a loss of insulin-normally sensitive tissue is what causes hyperglycemia (also
known as insulin resistance). When an autoimmune condition such as IDDM is present, the pancreas
cells' limited ability to produce insulin results in hyperglycemia. Both types have different
approaches to treating the illness and its side effects.

5. What methods provide a high degree of specificity for estimating glucose?

 Enzymatic methods offer the highest level of glucose specificity, making them excellent for
estimating actual blood glucose. Only blood plasma or serum is utilized in this technique. If serum or
plasma is prepared within 30 minutes of the glucose collection, the glucose is stable for 24 hours at
2-8°C.

Data and Results (Demographic Information’s of Patient):

ABSORBANCE OF UNKNOWN: 0.020
ABSORBANCE OF STANDARD: 0.025
Computations: put your final answer in a box:
Glucose(mg/dl) = Au/As x 100
0.020/0.025 x 100
0.8 x 100

= 80

Interpretations:
Within the reference range