Basically,in regenerative medicne, we have three regenerative technologies

working together to fully maintain our vital human organs. Restoring and rejuvenating the stem cell population is
Replenish: Stem cells, the regenerative engine of the body rejuvenating yourself.
Rejuvenate: Blood molecules (PRP W/Cytokines (parabiosis).
Replace: Organ regeneration and bioprinting

One option to accomplish this restoration and rejuvenation is to extract and concentrate your own autologous adult stem cells from
places like your adipose (or fat) tissue or bone marrow.
The nine hallmarks of aging
Genomic instability Cellular processes cause damage to genes, expose to increased radiation or free radicals, known to accelerate aging.
Telomere attrition Telomeres shorten as DNA replicates; if a reaches a critical shortness, cell stop dividing, increase incidence of disease.
Epigenetic alterations Environmental factors will change how genes are expressed
Loss of proteostasis: Different proteins in the body no longer fold and function as they are supposed to, resulting in diseases (Neuro/cancer)
Nutrient levels can influence metabolic pathways. The affected are proteins like IGF-1, mTOR, sirtuins, and AMPK.
Deregulated nutrient-sensing:
Changing levels of these proteins’ pathways has implications on longevity.
Mitochondrial dysfunction Mitochondria begin to decline in performance as we age. Decreased performance results in aging.
Cellular senescence: Cells age, stop working and cannot be removed from the body. They build up and typically cause increased inflammation.
Stem cell exhaustion As we age, our supply of stem cells begins to diminish as much as 100 to 10,000-fold in different tissues and organs.
Altered intercellular communication: Communication mechanisms that cells use, disrupted as cells age, resulting in decreased information cells to cell.

Aging is a balance between entropy (aging or slow breaking down of tissue) and rebuilding (stem cells).

Blood molecules (PRP)

Source Type Product Indication Principal Activity Harvesting
PRP Longevity Wrinkles, Homing, Angiogenesis, grow tisuees, Blood (20cc) + Centrifuge
Blood Products PRP Cytokine Longevity Regeneration tissue, antiinflammatory, Stimulate Blood (40cc) + Centrifuge + clot + Break
Monocytes Treat disease Blood (80cc) + Filter

CELL THERAPY
Source Type Advantages Disadaentages Cell Source Harvesting Technique
High availability and accessible. Inferior osteogenic and chondrogenic potential in Direct excision during
Subcutaneo
Stem cell isolation of up to 500 times more than BM. comparison to BM-MSCs. surgery, and Liposuction
us white
Cells proliferate faster than BM-MSCs Cell yield and differentiation potential is dependent (e.g. Coleman technique);
adipose
Adipose Tissue (AT) The immunosuppressive effects are stronger than on donor characteristics (i.e., age).
tissue from
comparatively easier,
those of BM-MSCs. safer, and considerably
trunk and
Secretion of angiogenic and antiapoptotic cytokines. larger amounts of
extremities
More prone to differentiate towards adipocyte lineage samples are accessible

The most extensively investigated. Considered to be Invasive and painful collection procedure. Bone
Bone marrow (trephine)
the gold standard. Procurement carries the risk of infection. marrow,
biopsy and aspiration;
The most common cellular source in clinical trials. Limited supply. usually
serious complications
Bone Marrow (BM) Established clinical history. Cell yield and differentiation potential is dependent from iliac,
such as pain, bleeding,
High chondrogenic and osteogenic potential. on donor characteristics (i.e., age). femur, tibia
infection and death exist
Less proliferative rate in comparison to BM-MSCs and
but rare
and UC-MSCs (mean doubling time of 4 ± 1 days). sternum
Safe and non-invasive collection procedure. UC-MSCs are less effective in inducing osteogenesis
Abundant supply. compared to BM-MSCs.
UC-MSCs do not age over passages (i.e., senescence).
Hypoimmunogenicity.
Umbilical Cord (UC) Lower risk of graft-versus-host diseases (GvHD).
Higher proliferation potential compared with BM
and AT (mean doubling time is 30 h).
Higher expansion and engraftment capacity than BM-
MSCs.