MCQs Based on Affinity Chromatography(BT19CME028,83,39)

Question 1: The Working Principal of Affinity Chromatography Depends on

A. Density
B. Interaction
C. Solubility
D. None of the Above

ANSWER: B Interaction

Question 2: Which one of the Following works as Support medium in the process

A. Ligand
B. Spacer
C. Matrix
D. None of the above

ANSWER: C Matrix

Question 3: The (1) prevents the non-specific interaction of the (2) with the (3)
itself.

A. (1) Spacer , (2) Ligand ,(3) Matrix

B. (1) Ligand , (2) Spacer ,(3) Matrix
C. (1) Matrix , (2) Ligand ,(3) Spacer
D. (1)Ligand , (2) Matrix , (3) Spacer

ANSWER: A (1) Spacer , (2) Ligand ,(3) Matrix

Question 4: The Ligand Binds with the target molecules ......

A. Ireversibly
B. Reversibly
C. With a ionic bond
D. None of the above

ANSWER: B Reversibly
Question 5: Which of the following is not a highly specific biological interaction to
be used in affinity chromatography ?

A. Cations-anions
B. Antigen-antibody
C. Enzymes-substrate
D. Receptor-ligand

ANSWER: A Cations-anions

Question 6: Which of this is not true while selecting a solid matrix or column
during affinity chromatography ?

A. The matrix should interact weakly with enzymes

B. The matrix should be based on inorganic compounds
C. The matrix should exhibit good flow property
D. The matrix should be highly porous

ANSWER: B The matrix should be based on inorganic compounds

Question 7: Affinity Chromatography can be used for

A. ..the separation of proteins according to their iso-electric points

B. ..the concentration of a compound
C. ..the purification of compounds from a complex matrix
D. ..the determination of protein molecular weights

ANSWER; B ..the concentration of a compound

Question 8: Arrange the working of Affinity chromatography

a. Setting up column
b. Target molecule binds to affinity ligand
c. Target molecule is eluted from the column
d. Disruption of interaction of ligand and target molecule
e. Sample is flowed into the column
f. Sample washed to remove unbound components
A. abdcfe
B. acbfed
C. aebfdc
D. Aebdfc

ANSWER: C aebfdc

Question 9: Which one is not a Advantage of Affinity Chromatography

A. Matrix being solid

B. Resolution of separation
C. Is a reproducible process
D. Takes a lot of skill to handle

ANSWER: D Takes a lot of skill to handle

Question 10: Which one is not a Application of Affinity Chromatography

A. Purify and concentrate a Enzyme Solution

B. Reduce the unwanted substance from the mixture
C. Separation based on cation-anion interaction
D. It is used to purify nucleic acid,antibodies,enzymes.etc

ANSWER: C Separation based on cation-anion interaction