Eppendorf Mar/31/2022

Document information

Software: realplex 2.2

File Name: admin\Gene Tech Lab\Gene Tech

220331
Printed by: admin
Created: Mar/31/2022 11:17
Serial No. Thermo Module: 6325 30070
Serial No. realplex Module.: 630211235

Acquisition Start Time: admin Mar/31/2022 11:34

Acquisition End Time: admin Mar/31/2022 13:39
Last updated: admin n/a

Background: ep clear strip 0.1ml+flat cap Jan/26/2011 16:28

Color Calibration: SYBR Nov/29/2010 08:16

Gene Tech 220331 Quantification Mar/31/2022 14:09

Inverted Data: OFF

Comment:

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 Eppendorf Mar/31/2022

Plate layout

1 2 3 4 5 6 7 8 9 10 11 12

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 Eppendorf Mar/31/2022

PCR Program

Program Header
Lid Temp 105 °C TSP Heated Lid Yes
Temp. Mode Standard Switch off lid at low block temp No
Impulse No Simulate Mastercycler gradient No

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 Eppendorf Mar/31/2022

Raw Data SYBR

Fluorescence Profile

Time [min]
A1, ASK - Control, SYBR
0 10 20 30 40 50 60 70 80 90 100 110 120
A2, ASK -ve, SYBR
4500 A3, ASK -ve, SYBR
4000 B1, SF - Control, SYBR
3500 B2, SF +ve, SYBR
B3, SF +ve, SYBR
3000
Fluorescence

C1, HNA C, SYBR

2500 C2, HNA +ve, SYBR
2000 C3, HNA +ve, SYBR
D1, FIA C, SYBR
1500
D2, FIA +ve, SYBR
1000 D3, FIA +ve, SYBR
500 E1, PC +ve, SYBR
0 E2, PC +ve, SYBR
E3, PC C, SYBR
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 F1, LAF -ve, SYBR
Cycle

Temperature Profile

100

80
Temperature [°C]

60

40

20

00
0 10 20 30 40 50 60 70 80 90 100 110 120
Time [min]

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 Eppendorf Mar/31/2022

Quantification SYBR

Pos Name Ct SYBR Ct Mean SYBR Ct Dev. SYBR Amount SYBR Target SYBR

A1 ASK - Control 31.08 -

A2 ASK -ve 23.94 23.48 0.66 -

A3 ASK -ve 23.02 23.48 0.66 -

B1 SF - Control 33.99 -

B2 SF +ve 35.71 36.13 0.60 -

B3 SF +ve 36.55 36.13 0.60 -

C1 HNA C 35.05 -

C2 HNA +ve 34.50 34.63 0.18 -

C3 HNA +ve 34.76 34.63 0.18 -

D1 FIA C 32.43 -

D2 FIA +ve 33.22 33.20 0.02 -

D3 FIA +ve 33.19 33.20 0.02 -

E1 PC +ve 36.09 35.40 0.97 -

E2 PC +ve 34.72 35.40 0.97 -

E3 PC C 34.08 -

F1 LAF -ve 23.79 24.96 1.65 -

F2 LAF -ve 26.13 24.96 1.65 -

F3 LAF C 34.08 -

G1 EJA +ve -

G2 EJA +ve 35.63 -

G3 EJA C 35.35 -

H1 TM -ve 21.73 27.38 7.99 -

H2 TM -ve 33.03 27.38 7.99 -

H3 TM C 24.46 -

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 Eppendorf Mar/31/2022

Amplification Plot

A1, ASK - Control, SYBR

A2, ASK -ve, SYBR
1000
A3, ASK -ve, SYBR
B1, SF - Control, SYBR
Fluorescence (norm)

B2, SF +ve, SYBR

B3, SF +ve, SYBR
100
C1, HNA C, SYBR
C2, HNA +ve, SYBR
C3, HNA +ve, SYBR
10 D1, FIA C, SYBR
D2, FIA +ve, SYBR
D3, FIA +ve, SYBR
E1, PC +ve, SYBR
1 E2, PC +ve, SYBR
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 E3, PC C, SYBR
Cycle Number F1, LAF -ve, SYBR

Threshold 111 (Noiseband)

Baseline automatic, Drift correction OFF

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