Faculty of Pharmacy

Department of Pharmaceutical Chemistry

Analytical Quality Control (PCC 402 / PC 511 / PHR 525)
Tutorial 8
Qualification of UV/Vis spectrophotometers

Limit of stray light

1) Give the specific expression for
a. The portion of light passing through solution which is not part of the normal λ
.
b. The detected light outside the bandwidth of the selected λ.
2) How many types of stray light can interfere in measuring absorbance. Which type
would seriously affect the magnitude of absorbance?
Give an expression to relate absorbance to stray light
3) Give an expression to relate absorbance to stray light at high concentration

4) Assuming constant transmittance at 1% of the incident light

Calculate absorbance at 0 % stray light & at 1.0 % stray light of the intensity of the
incident light. Compare and comment
5) a. At constant transmittance, the higher the stray light
i. The lower the absorbance
ii. The higher the absorbance
iii. The higher the transmittance
iv. No correlation
b. The preferred range of measuring absorbance is
i. 0.1 – 1.3
ii. 0.3 – 1.0
iii. 0.0 – 1.0
iv. None of these
c. Stray light can be tested by a solution of
i. KCl
ii. NaI
iii. NaNO2
iv. All of these
6) How can you test for the presence of stray light?