No Type/Name Region Ligand Id/Refere Evidence Note

nce
1 CHAIN 1-400 "PRO_000 "MAP kinase-activated protein kinase
0086288" 2"

2 DOMAIN 64-325 evidence="ECO:0000255| "Protein kinase"

PROSITE-
ProRule:PRU00159"

3 REGION 1-43 evidence="ECO:0000256| "Disordered"

SAM:MobiDB-lite"

4 REGION 328-364 "ECO:0000250" "Autoinhibitory helix"

5 REGION 366-390 "p38 MAPK-binding site"

6 MOTIF 356-365 "Nuclear export signal (NES)"

7 MOTIF 371-374 "Bipartite nuclear localization signal 1"

8 MOTIF 385-389 "Bipartite nuclear localization signal 2"

9 COMPBIAS 10-43 "ECO:0000256|SAM:Mob "Pro residues"

iDB-lite"

10 ACT_SITE 186 "Proton acceptor"

11 BINDING 70-78 "ATP" "ChEBI:C "ECO:0000255|PROSITE-

HEBI:306 ProRule:PRU00159"
16"

12 BINDING 93 "ATP" "ChEBI:C "ECO:0000255|PROSITE-

HEBI:306 ProRule:PRU00159"
16"

13 BINDING 139-141 "staurosp "ChEBI:C

orine" HEBI:574
91"

14 MOD_RES 9 "ECO:0000269|PubMed:8 "Phosphoserine"

846784"

15 MOD_RES 25 "ECO:0000269|PubMed:8 "Phosphothreonine"

846784"

16 MOD_RES 222 "ECO:0000269|PubMed:8 "Phosphothreonine; by MAPK14"

846784"

17 MOD_RES 272 "ECO:0000269|PubMed:8 "Phosphoserine; by MAPK14"

846784"

18 MOD_RES 328 "ECO:0000250" "Phosphoserine; by autocatalysis"

19 MOD_RES 334 "ECO:0000269|PubMed:8 "Phosphothreonine; by MAPK14"

846784,
ECO:0007744|PubMed:23
186163,
ECO:0007744|PubMed:24
275569"
20 CROSSLNK 353 "ECO:0000269|PubMed:2 "Glycyl lysine isopeptide (Lys-Gly)
1131586" (interchain with G-Cter in SUMO)"

21 VAR_SEQ 354-400 "VSP_004 "ECO:0000303|PubMed:8 "EEMTSALATMRVDYEQIKIKKIED

910" 179591" ASNPLLLKRRKKARALEAAALAH
-> GCLHDKNSDQATWLTRL (in
isoform 2)"

22 VARIANT 173 "VAR_040 "ECO:0000269|PubMed:1 "A -> G (in dbSNP:rs35671930)"

753" 7344846"

23 VARIANT 361 "VAR_040 "ECO:0000269|PubMed:1 "A -> S (in dbSNP:rs55894011)"

754" 7344846"

24 MUTAGEN 93 "ECO:0000269|PubMed:1 "K->R: Kinase defective mutant,

4517288" abolishes activity."

25 MUTAGEN 207 "ECO:0000269|PubMed:8 "D->A: Kinase defective mutant,

846784" abolishes activity."

26 MUTAGEN 222 "ECO:0000269|PubMed:1 "T->A: Strong decrease in kinase

4517288, activity."
ECO:0000269|PubMed:15
014438,
ECO:0000269|PubMed:88
46784"

27 MUTAGEN 222 "ECO:0000269|PubMed:1 "T->D: Mimicks phosphorylation state,

4517288, leading to slight increase of basal
ECO:0000269|PubMed:15 kinase activity."
014438,
ECO:0000269|PubMed:88
46784"

28 MUTAGEN 222 "ECO:0000269|PubMed:1 "T->E: Mimicks phosphorylation state

4517288, and constitutive protein kinase activity;
ECO:0000269|PubMed:15 when associated with E-334."
014438,
ECO:0000269|PubMed:88
46784"

29 MUTAGEN 272 "ECO:0000269|PubMed:8 "S->A: Strong decrease in kinase

846784" activity."

30 MUTAGEN 272 "ECO:0000269|PubMed:8 "S->D: Mimicks phosphorylation state,

846784" leading to slight increase of basal
kinase activity."

31 MUTAGEN 334 "ECO:0000269|PubMed:1 "T->A: Slight decrease in kinase

4517288, activity."
ECO:0000269|PubMed:15
014438,
ECO:0000269|PubMed:88
46784"

32 MUTAGEN 334 "ECO:0000269|PubMed:1 "T->D,E: Mimicks phosphorylation

4517288, state, leading to elevated basal kinase
ECO:0000269|PubMed:15 activity."
014438,
ECO:0000269|PubMed:88
46784"
33 MUTAGEN 334 "ECO:0000269|PubMed:1 "T->E: Mimicks phosphorylation state
4517288, and constitutive protein kinase activity;
ECO:0000269|PubMed:15 when associated with E-222."
014438,
ECO:0000269|PubMed:88
46784"

34 MUTAGEN 353 "ECO:0000269|PubMed:2 "K->R: Induces decreased sumoylation

1131586" and increase in protein kinase activity."

35 CONFLICT 116 "ECO:0000305" "H -> D (in Ref. 5; CAA53094)"

36 CONFLICT 247-248 "ECO:0000305" "WS -> LV (in Ref. 5; CAA53094)"

37 HELIX 45-47 "ECO:0007829|PDB:1NX

K"

38 STRAND 48-50 "ECO:0007829|PDB:1NX

K"
39 HELIX 59-61 "ECO:0007829|PDB:3M2
W"

40 STRAND 63-73 "ECO:0007829|PDB:3M2

W"

41 STRAND 76-83 "ECO:0007829|PDB:3M2

W"

42 TURN 84-86 "ECO:0007829|PDB:3M2

W"

43 STRAND 89-96 "ECO:0007829|PDB:3M2

W"

44 HELIX 99-111 "ECO:0007829|PDB:3M2

W"

45 STRAND 114-117 "ECO:0007829|PDB:3FY

K"

46 STRAND 120-128 "ECO:0007829|PDB:3M2

W"

47 STRAND 131-138 "ECO:0007829|PDB:3M2

W"

48 STRAND 143-145 "ECO:0007829|PDB:1NX

K"

49 HELIX 146-152 "ECO:0007829|PDB:3M2

W"

50 HELIX 154-156 "ECO:0007829|PDB:6T8X

"

51 HELIX 160-179 "ECO:0007829|PDB:3M2

W"

52 HELIX 189-191 "ECO:0007829|PDB:3M2

W"
53 STRAND 192-198 "ECO:0007829|PDB:3M2
W"

54 STRAND 203-205 "ECO:0007829|PDB:3M2

W"

55 STRAND 212-214 "ECO:0007829|PDB:3WI6

"

56 HELIX 227-229 "ECO:0007829|PDB:6T8X

"

57 HELIX 232-234 "ECO:0007829|PDB:1NX

K"

58 HELIX 239-242 "ECO:0007829|PDB:3M2

W"

59 HELIX 243-258 "ECO:0007829|PDB:3M2

W"

60 STRAND 264-266 "ECO:0007829|PDB:4TY

H"

61 STRAND 267-269 "ECO:0007829|PDB:1KW

P"

62 HELIX 275-281 "ECO:0007829|PDB:1NX

K"

63 STRAND 283-285 "ECO:0007829|PDB:2OZ

A"

64 HELIX 288-291 "ECO:0007829|PDB:3M2

W"

65 STRAND 292-294 "ECO:0007829|PDB:6T8X

"

66 HELIX 296-305 "ECO:0007829|PDB:3M2

W"

67 TURN 310-312 "ECO:0007829|PDB:3M2

W"

68 HELIX 316-320 "ECO:0007829|PDB:3M2

W"

69 HELIX 323-326 "ECO:0007829|PDB:3M2

W"

70 HELIX 328-330 "ECO:0007829|PDB:3M2

W"

71 STRAND 335-337 "ECO:0007829|PDB:3M4

2"

72 HELIX 338-344 "ECO:0007829|PDB:3M2

W
73 HELIX 346-348 "ECO:0007829|PDB:3M2
W"

74 HELIX 349-363 "ECO:0007829|PDB:3M2

W"

75 TURN 375-377 "ECO:0007829|PDB:2OK

R"

76 HELIX 381-389
"ECO:0007829|PDB:2OK
R"