United States Department of Agriculture

Research, Education, and Economics

Agricultural Research Service

UNITED STATES DEPARTMENT OF AGRICULTURE

Agricultural Research Services
Washington, D.C.

and

INSTITUTO PARAGUAYO DE TECNOLOGÍA AGRARIA

(IPTA)
San Lorenzo, Paraguay

and

CÁMARA PARAGUAYO DE EXPORTADORES DE CEREALES Y OLEAGINOSAS

(CAPECO)
Asunción, Paraguay

NOTICE OF RELEASE OF DS5-67

The Agricultural Research Service of the U.S. Department of Agriculture, the Instituto
Paraguayo de Tecnología Agraria, and the Cámara Paraguaya de Exportadores de Cereales y
Oleaginosas announce the release of soybean [Glycine max (L.) Merr.] DS5-67, an improved
maturity group (MG) V non-GMO germplasm line with resistance to soybean rust (SBR) caused
by Phakopsora pachyrhizi Syd. Resistance to SBR was derived from MG VIII ‘Ankur’ (PI
462312). This is the first improved MG V U.S. germplasm release with Rpp3 resistance to SBR.
Scientists participating in the development of DS5-67 were J.R. Smith, R.D. Frederick, A.M.
Gillen, J.D. Ray, D.R. Walker, and G. Cai (USDA-ARS, Stoneville, MS; Ft. Detrick, MD;
Urbana, IL and West Lafayette, IN), E. Rodriguez (CAPECO, Asunción, Paraguay), and W.
Morel (IPTA, Capitán Miranda, Paraguay). DS5-67 is being released because it is competitive
under rust pressure, has Rpp3 resistance to SBR and Rps1k resistance to Phytophthora root and
stem rot (PRSR) caused by Phytophthora sojae, and has moderately high protein content. It will
be valuable for improving SBR and PRSR resistance, while increasing seed protein content.

DS5-67 was derived from a single F5 plant from the cross ‘Williams 82’ × Ankur made at
Stoneville, MS in 2004. F1 and F2 plants were grown during the winters of 2004-05 and 2005-
06, respectively, at the USDA-ARS Tropical Agriculture Research Station near Isabela, PR. A
total of 110 F2:3 families were developed and used in a study that determined that the genomic
location of the Rpp3 rust resistance gene was on chromosome 6. DS5-67 was the 67th family
assayed at Ft. Detrick, MD, where it was found to be homozygous resistant with a Reddish-
Brown (RB) reaction, indicative of partial resistance to the IN73-1 isolate. Based on the above

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assays, remnant F3 seeds of family 67 were grown in Stoneville in 2006, where single F3 plants
were selected. Similarly, F4 and F5 single plants were selected at Stoneville in 2007 and 2008,
respectively. In 2009, the F5-derived 6-line DS5-67 was bulk harvested at Stoneville.

DS5-67, Ankur, and Williams 82 were assayed at Ft. Detrick, MD in 2009, 2010, and 2012 with
multiple SBR isolates. Williams 82 had a Tan-colored (TAN) susceptible reaction to all isolates,
whereas DS5-67 and Ankur had RB reactions to SA01-1, LA04-1, and IN73-1, but TAN
reactions to CO04-1, VT05-1, ZM01-1, and TW80-2. DS5-67 was tested with an unusually
virulent SBR field population in Quincy, Florida in 2012, where it had a TAN reaction. Prior
and subsequent Quincy SBR populations were considerably less virulent on Rpp3 resistance,
indicating that DS5-67 may still have effective SBR resistance in the southeastern U.S.

Seed yield was measured for DS5-67 in side-by-side fungicide-treated (triazoles and
strobilurins)/non-treated split plots under natural SBR pressure in 2012 and 2013 at Capitán
Miranda, Paraguay. Averaged across years, DS5-67 yielded 2,129 kg/ha in treated plots and
1,699 kg/ha in untreated plots (a 20% yield reduction due to SBR) compared to MG V cultivar
Jake, which yielded 3,463 kg/ha in treated plots and 1,709 kg/ha in untreated plots (a 51% yield
reduction due to SBR). In the same trials, G01-PR16 (with resistance derived from Hyuuga),
‘CM422’ (Rpp4), and ‘Dillon’ suffered 22%, 26%, and 51% yield loss, respectively, from SBR.

At Stoneville over 4 years (2010-2013), DS5-67 yielded less than Jake (2,224 kg/ha vs. 3,998
kg/ha), was one day later in maturity, was taller (59 cm) than Jake (52 cm), was similar in seed
germination (76% and 78%, respectively), and had the same lodging score (1.0) as Jake.
Averaged over 2011-2013, seed size of DS5-67 was smaller (13.0 g/100 seed) than that of Jake
(14.2 g/100 seed). In 2019, DS5-67 averaged 38% protein (51.2% estimated protein meal) and
18.7% oil (13% moisture), whereas seed of ‘AG5335’ averaged 34.6%, (48.3%), and 20%,
respectively. In the USDA Uniform Soybean Tests, Southern States-Preliminary Tests averaged
across 20 locations over 2 years (2011 and 2012), DS5-67 yielded less than ‘AG5606’ (2,526
kg/ha versus 3,554 kg/ha), but its seed contained 36.8% protein and 18.4% oil (13% moisture),
while seed of AG5606 contained 35.1% protein and 19.1% oil).

DS5-67 has white flowers, tawny pubescence, brown pod walls, yellow seed coats, and light
brown hila. It carries the Rpp3 gene for resistance to SBR, is resistant to stem canker (Diaporthe
phaseolorum var. meridionalis), has the Rps1k gene for resistance to PRSR races 1, 3, 4, 7, and
17, and may be segregating for an additional Rps gene segregating for resistance to race 25.
DS5-67 is susceptible to bacterial pustule (Xanthomonas axonopodis pv. glycines) and
Cercospora leaf blight (Cercospora flagellaris, formerly C. kikuchii).

A limited quantity of seed is available from J.R. Smith (USDA-ARS, Crop Genetics Research
Unit, PO Box 345, Stoneville, MS 38776, rusty.smith@usda.gov). Seed of this release will be
deposited in the National Plant Germplasm System, where it will be available for research
purposes, including development and commercialization of new cultivars. It is requested that
appropriate recognition be made when DS5-67 contributes to the development of new
germplasm lines and cultivars.

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