A. Study the microscope and label the parts on the figure below.

Be able to point out correctly

to the instructor all the parts of the microscope and their uses before labeling
the figure.
Eyepieces (Ocular Lens) -
magnifies the image of the Observation tube - hold the
specimen eyepiece.

Revolving Nosepiece - enables the MicroscopeFra

microscope user to quickly change me
objective lens magnifications and while
keeping the specimen centered.
Main Switch - turns
the illuminator off
and on

Objetive lens
Stage - is where the specimen
is placed Light Intnsity knob
Specimen holder

Iris Diaphgram knob

Course Focus knob
Condenser Fine Focus knob-
Substage light - located in the Fine focus moves
base, the light passes directly the stage in smaller
upward through the increments to
Base - is provide a clear
microscope. the main view of the
support of specimen
the
microscope
Objective lens - consists of several lenses to magnify an object and
project a larger image.

Specimen holder- allows the slide to be easily inserted or

removed from the microscope.
Light Intnsity knob - adjusts the intensity of the light
Iris Diaphagram knob - the arm attached to the base of the passing through the microscope
condenser that regulates the amount of light passing through the
condenser.
Course Focus knob - moves the stage to provide
general focus on the specimen
Condenser - These are lenses that are used to collect
and focus light from the illuminator into the
specimen
Microscope Frame -allows attachment of the focus wheels and the
stage to the microscope.
B. Questions. Answer the following in 7-10 sentences only. (5 point-credit each)

1. When mounting a specimen on a slide, always cover it with a cover glass before examining
under a microscope. Why?

ANS : We should always cover it with a cover glass before examining under a microscope, when
mounting a specimen on a slide. Because the cover glass protects the microscope and prevents the
slide from drying out when it's being examined. It helps keep what you are looking at getting on the
lens of your microscope. Also to keep solid specimens pressed flat, and liquid samples shaped into a
flat layer of even thickness. Cover glass will also give the viewer better control over the specimen.
Without a cover glass the objective lens on the microscope would get dirty.

2. Why is it always necessary to focus with the fine adjustment in high magnifications?

ANS : It is always necessary to focus with the fine adjustment in high magnifications to sharpen the
focus of the image. Also the fine adjustment only moves the tube a teeny tiny bit and very slowly so
you don’t have great risk of damaging the slide or lens. Because if you use course adjustment when
using high magnifactions, it could break the slide or the microscope. Using course adjustment the
body tube of the microscope will hit the microscope slide and cause the slide to break or crack.

3. Explain the proper technique for transporting the microscope.

ANS : The proper technique for transporting the microscope, you should always use one hand to
support the base and the other to hold the arm of the microscope. Always carry it gently and place
it down carefully, so that no damage any of the more delicate components. Remember that you
should never handle the lenses, and be careful not to hold it by the stage. In order to avoid any trip
hazards, the wire should also be softly wrapped slightly above the base, the stage should be in its
lowest positon and the lowestpower objective should be in place.

4. Name some outstanding scientists who contributed to the perfection of the microscope.

ANS : A Dutch father-son team named Hans and Zacharias Janssen invented the first so-called
compound microscope in the late 16th century, but in 1609 Galileo Galilei perfected the first device
known as a microscope.
C. The following statements are true or false. If true, write T on the answer blank. If false, correct the
statement by writing on the blank the proper word or phrase to replace the one that is underlined.

1. The microscope lens may be cleaned with any soft tissue.

ANS : False - with grit-free lens paper

2. The microscope should be stored with the oil immersion lens in

position over the stage.
ANS : False – lowest power

3. When beginning to focus, use the lowest-power lens.

ANS : True

4. When focusing, always focus toward the specimen.

ANS : True

5. A coverslip should always be used with wet mounts and the high
power and oil lenses.

ANS : True
Match the microscope structures in column B with the statements in column A that
identify or describe them.
Column A
1. platform on which the slide rests for viewing
ANS : j. stage
2. used to increase the amount of light passing through the specimen

ANS : b. condenser

3. secure(s) the slide to the stage

ANS : e. mechanical stage

4. delivers a concentrated beam of light to the specimen

ANS : d. iris diaphragm
5. used for precise focusing once initial focusing has been done
ANS : c. fine adjustment knob
6. carries the objective lenses; rotates so that the different objective lenses can be brought
into position over the specimen
ANS : f. nosepiece

Column B

a. coarse adjustment knob

b. condenser

c. fine adjustment knob

d. iris diaphragm

e. mechanical stage

f. nosepiece

g. objective lenses
h. ocular
i. spring clips
j. stage