PROPOSED SCHEMATIC DIAGRAM FOR HPC REPEATABILITY IN WATER
CULTURE FOR INOCULATION
SAMPLE INOCULUM PREPARATION
ANALYSIS PROPER (DILUTION)
100
Neat Sx
ANALYSIS PROPER (INOCULATION)
Inoculate appropriate
volume of inoculum to ten
(10) sets of duplicates for
repeatability check
1000mL Sterile distilled water
1mL
• Transfer 1mL of sample inoculum
to 1,000 mL of Sterile distilled
water.
This will serve as 100.
• Properly mix sample inoculum for
2 minutes.
100 bottle (Neat sample) will be used
1mL for inoculation of 1:1 sample and 1:10
sample.
Diluted sample on the other hand will
be used for inoculation of 1:100 and
1:1000 sample.
99mL diluent
100
Inoculate appropriate
1mL 0.1mL
volume of inoculum to ten
(10) sets of duplicates for
repeatability check
1:1 (100) 1:10 (10-1)
 PROPOSED SCHEMATIC DIAGRAM FOR HPC REPEATABILITY IN WATER

For 10-2 and 10-3 Dilutions refer to the procedure below:

1:100 Diluted Sx

1mL 0.1mL
Inoculate appropriate Inoculate appropriate
volume of inoculum to ten volume of inoculum to ten
(10) sets of duplicates for (10) sets of duplicates for
repeatability check repeatability check

1:100 (10-2) 1:1000 (10-3)

Pour 15 – 20mL of Tryptone glucose yeast agar (TGYA) or Plate Count Agar (PCA), swirl

Swirl and let cool

Let cool and Incubate invertedly at 35oC for 48 hours ± 3 hours