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kineticII Lab1
kineticII Lab1
Title
Aim:
- To examine and confirm Beer's law using the sodium salicylate solution as an example.
- To find out the equation used to describe the straight line of the calibration curve.
- To determine the concentration of unknown sample from the standard calibration curve of sodium salicylate.
- Cuvettes. - Dropper.
Procedure:
1 - Calculate the required volume for other concentrations.
2 - Measure the Abs for each solution & for unknown sample.
3 - Present Data on graph paper and find out the equation of the calibration curve for sodium salicylate at 310 nm.
4 - Determine the concentration of unknown sample from the standard calibration curve of sodium salicylate.
Results:
Absorbance Concentration
2.5 0.225
5 0.239
10 0.274
15 0.311
20 0.35
Calculations:
0.025% =
0.025 g 100 ml
Xg 1 ml
6
(0.025 x 10 ) / 100 = 250 ug/ml
.............................................................................
B:
y = 0.0072x + 0.2044
0.276 = 0.0072x + 0.2044
= 9.94 ug/ml
Conclusion: