Study of Cleaning Agents Performance

in Removing Pork Substance on Food

Equipment After Ritual Purification

by:
Rosy Hutami, STP, M.Si1
Dr. Ir. Joko Hermanianto2
Dr. Nancy Dewi Yuliana, STP, M.Sc2,3
1 Department of Food Technology and Nutrition, Faculty of Halal Food
Science, Djuanda University, Bogor, Indonesia
2Department of Food Science and Technology, Faculty of Agricultural

Technology, Bogor Agricultural University Bogor, Indonesia

3Halal Science Center, Bogor Agricultural University Bogor, Indonesia

The 2nd Bogor International Conference for Applied Science

Introduction

 Halal food includes not only ingredients, but also including

the purity of the equipment it uses.
 There are often found clean food equipments, but not pure
according to sharia. This food equipment must be purified by
shariah purification in order not to contaminate and alter the
halal status of the food that is in contact with it.
 However, ritual purification, which generally uses soil as one
of its ingredients, can sometimes damage the equipments

 Thus, scholars' agreements in some countries allow the use of

other cleaning agents to replace the soil.
 This study is the first study in Indonesia that compared the
ability of diver cleaning agents in removing pork substance.
The 2nd Bogor International Conference for Applied Science
 Purpose

The purpose of this study was to show the performance of

cleaning agents including earth, acid, alkaline, and
detergent in removing DNA and fatty acid residue on food
equipment after ritual purification.

Methods
Target Analysis Methods of Analysis
Pork DNA residue Polymerase Chain
Reaction (PCR) with
cytochrome B as
target gene
Pork Fatty Acid Gas chromatography-
residue mass spectrometry
(GC-MS) analysis

The 2nd Bogor International Conference for Applied Science

Result and DIscussion
Pork DNA Residue Analysis by Polymerase Chain Reaction

Amplifikasi Gen Sitokrom b (cyt b) pada Sampel

398 pb

Picture 1 P : Pork possitive control, N: non template control, 1: earth

solution 0.5% purification, 2: commercial detergent solution 0.5%
purification, 3: clay detergent solution 0.5% purification, 4: HCl solution
0.5% purification, 5: HNO3 solution 0.5% purification, dan 6: NaOH solution
0.5% purification
The 2nd Bogor International Conference for Applied Science
 Result and DIscussion
Pork Fatty Acid Residue Analysis by Polymerase Chain Reaction
pork negative
possitive control
control

commercial clay
detergent detergent

The 2nd Bogor International Conference for Applied Science

 Result and DIscussion
Pork Fatty Acid Residue Analysis by Polymerase Chain Reaction

HCl HNO3

NaOH Earth

The 2nd Bogor International Conference for Applied Science

Conclusion

Earth solution was the best cleaning agent in

removing pork DNA residue and commercial
detergent was the best cleaning agents in
removing fatty acid residue.

-Thank you -

The 2nd Bogor International Conference for Applied Science