Determination of Peroxide Value

I. Introduction
Peroxide values is a measure of the peroxides contained in the soil. The
peroxides present are determined by titration against thiosulfate in the presence of KI
and by using starch soluble as its indicator.
II. Apparatus and Equipment
 Balance Machine
 Burette with stand
 Graduated Cylinder
 Erlenmeyer flask
 Funnel
 Beaker
 Hot plate

III. Reagents
 Acetic acid
 Chloroform
 Sodium thiosulfate
 Starch Soluble
 Potassium Iodine

IV. Chemical Preparations

a. Acetic acid and Chloroform(3:2)
 Mix 3 volume of acetic acid and 2 volume of chloroform.
b. 1% Startch Solution
1. Weigh 1 gram of soluble starch and transfer the weighted starch in
50mL boiling water.
2. Continue boiling and stirring until a transparent solution obtained.
3. Filter the solution using a filter paper to obtained the clear solution.

c. Potassium Iodide Solution

1. Dissolve 10g of potassium iodide in distilled water to make 100mL
2. Store in light resistant container

d. 0.1N Sodium thiosulfate Preparation

1. Dissolve 0.25 g sodium thiosulfate + 0.02g of sodium carbonate in enough
recently boiled and cooled water to make 250mL
0.1N Sodium thiosulfate Standardization
1. Dissolve 0.125g potassium dichromate in 25mL water in io
 V. Sample Preparation
Weigh 5 grams of the sample in a 250mL conical flask
+
30mL of acetic acid and chloroform. Shake and mix the sample
+
0.5mL of Saturated potassium iodide and shake
Note: Allow the solution to stand for 1 min.
+
30mL of distilled water and titrate with standard thiosulfate solution
+
Thiosulfate solution until the color of titrated solution becomes light yellow
+
Add 1mL of Starch indicator solution and continue the titration till the disappearance of blue
color
+
Carry out a blank determination without the sample
+
Note down the titration value

VI. Calculation

[1000 (VT – VB)N]/W

Where:
VT=Volume of Sample Titration (mL)
VB= Volume of Blank Titration (mL)
N= Normality of Sodium thiosulfate solution
W= is the weight (g)
 Determination of Acid Value

I. Introduction
The acidity of fats and fixed oils may be expressed as the number of mL of 0.1 N
alkali required to neutralize the free acids in 10.0f substance. The a

II. Apparatus an Equipment

 Balance Machine
 Burette with stand
 Graduated cylinder
 Conical flask
 Hot plate

III. Reagents
 Sodium Hydroxide
 Phenolphthalein
 Ethanol

IV. Chemical Preparation

a. Preparation of Phenolphthalein Indicator

1. Dissolve 2g of Phenolphthalein indicator powder into 50 mL of distilled water.
2. Transfer the solution into a conical flask and make 100 m

b. Preparation of Potassium Hydroxide Phthalate

1. Dissolve 5.1 grams of KHP into 200 ml of distilled water and make a final volume of 250
mL..
c. Preparation of 0.1 N Sodium Hydroxide Preparation
1. Dissolve 1g Sodium Hydroxide pellets into 200mL of distilled water and make a final
volume of 250mL.
2. Transfer a 10mL volume of KHP to a conical flask then add 2-3 drops of
phenolphthalein.
3. Titrate the solution with 0.1 N NaOH until faint pink color appears.

d. Standardization of 0.1N Sodium Hydroxide Solution

1.
V. Sample Preparation

Weigh 10 grams of the sample. Set aside.

Measure 50mL of ethanol and pour it into the conical flask

+
2-3 drops of Phenolphthalein indicator solution and shake
+
0.1N NaOH dropwise until a light pink solution is formed
(To neutralize the ethanol)

Add neutralize ethanol to a flask containing the sample. Shake the flask to mix the solution.
 Boil the solution until the sample is dissolved completely. Shake the flask frequently.

Take 0.1 N NaOH (standardized) in a burette

Start titration by adding 1 mL of phenolphthalein indicator. Pink solution indicates the end point
of the titration.

VI. Calculation:

56.11V × N/W

Where:
56.11=MW of Potassium Hydroxide
V=volume in mL
N= Normality of KOH/NaOH
W=Weight in grams