4/3/2012

Running GC

Running GC
1. Launch the
“TotalChrom”
Software
2. Click on “Run”
3. Select “Take Control…”
4. Select “Clarus 580”
5. Click “OK”

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Running GC
• The GC touch screen panel should show
that the GC is in external control.
• Once in external control:
• Build Method
• Build Sequence

Build Method

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Build Method

1. Click on “Method”
button

Build Method

2. Select “Create
new method”
3. Click on “OK”

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Build Method
4. Type in a description
5. Click “Next”

6. Type in a header text

7. Click “Next”

• The Data Acquisition

window should look like the
following
8. Click “Next”

Build Method

• Autosampler tab should

look like the following
• Adjust as you see fit
• What is shown is a good
standard
9. Click “Oven/Inlets” Tab

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Build Method

10. Adjust the Oven Ramp

program as directed.
• Make sure to hit enter
on the keyboard after
every entry
11. Adjust the “Injector A”
temperature. Typically this
is set around 225 C.
12. Click on “Carrier” Tab

Build Method

13. Adjust the Carrier tab as

directed.
• Typical settings are
shown.
14. Click on “Detectors” Tab

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Build Method

15. Adjust the Detectors tab as

directed.
• Typical settings are
shown.
16. Click on “Next”

(No Need to Mess with)

Build Method

Click “Next”

Click
Click “Next” “Finish”

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Build Method

17. File  Save

18. Create a unique name
19. Click on “Save”
20. Close Method window

Build Sequence

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Build Sequence

1. Click on “Sequence”
button

Build Sequence

2. Select “Create new sequence”

3. Click on “OK”

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Build Sequence

4. Select the listed options

5. Click on “OK”

Build Sequence
6. Type in a unique name
7. Type in which vial to be used
8. Select a method (brings up a
new window)
9. Click on “Add+Close” if all vials
are added or click on “Add” to
add more vials

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Build Sequence

10. File  Save

11. Create a unique name
12. Click on “Save”
13. Close Sequence window

Running Sequence

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Running Sequence

1. Click on “Setup” button

Running Sequence

1. Select a sequence (new

window will open / not
shown)
2. Click OK

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Running Sequence
• The GC will take some
time to upload the
sequence information,
equilibrate, and do pre-
run operation
• You will know that the
sequence upload properly
because the Instrument-
Hands On and
Instrument-Modify will no
longer be grayed out
• Once the everything is
ready the lettering here
should be in green.
• I/F will read Ready
3. Click on Run
4. Start Run

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