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Microbial Genetics: Genome organization in bacteria; Plasmid - Types of plasmids, Plasmid

replication and partitioning, Host range, plasmid-incompatibility, plasmid amplification,


Regulation of copy number, curing of plasmids; Mechanisms of Genetic Exchange -
Transformation - Discovery, mechanism of natural competence; mapping through co-
transformation frequencies; Conjugation - Discovery, mechanism, Hfr and Fʹ strains, Interrupted
mating technique and time of entry mapping; Transduction - Generalized transduction,
specialized transduction, LFT & HFT lysates, abortive transduction.

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