SCIENCE

FOURTH YEAR - MYP

FIRST YEAR - BGE
2022 – 2023

Name: Lia Andrade, Jose Pineida, Zahid Cabrera Grade: 1B

Teacher: Marcela Cisneros Flores

DIRECTIONS
- Read carefully the instructions and the evaluation criteria.
- Academic dishonesty is penalized with 0 according to Arts 226, 330 of the LOEI.

SCIENCE LAB REPORT

EVALUATION CRITERIA

Theoretical Analysis Evaluation Communication total

framework
/2 /2 /2 /2 /8

1. TOPIC: Microscopy and Cells

2. OBJECTIVES:

To describe the parts and functions of the compound light and dissecting microscope.
To state the steps in proper order for bringing the cell’s image into focus with the compound light
microscope.
To calculate the total magnification and the estimated size of the Cell’s image.
To identify the differences between cells and classify them.

Research Questions: What is the estimated size of cells?

Hypothesis: The size of the cells is very small to be seen by naked eye, their size can vary from
one to a hundred micrometers

3. THEORETICAL FRAMEWORK:

A microscope slide is a thin sheet of glass used to hold objects for examination under a
microscope. A standard microscope slide is 75 x 25 mm and 1.2 mm thick. A range of other
sizes is available for various special purposes. A simple light microscope manipulates how
light enters the eye using a convex lens, where both sides of the lens are curved outwards.
When light reflects off of an object being viewed under the microscope and passes through
the lens, it bends towards the eye. This makes the object look bigger than it actually is. A
light microscope can be used to determine the actual cell size. Using a light (optical)
microscope, you can easily determine the cell size.

4. MATERIALS:

Microscope:(PARTS)
Objective lenses (4x, 10x, 40x ,100x)
Immersion oil
Bacteria, plant and animal cell samples

5. PROCEDURE:
 ✓ Choose Bacteria Gram Stain Mix

✓ Focus the specimen in 4x

✓ See the specimen in 10x, 40x, 100x

✓ Repeat the same process using Plant cells slide and one more time using Human
Blood cells

6. SAFETY PERSONAL AND ENVIROMENTAL MEASURES:

The first suggestion for this experiment is that if you're going to the lab, you should wear a
gown that hasn't been used outside of the lab, tie it tightly, and cover your hands with
gloves. Long hair should also be pulled back.

7. DATA COLLECTION AND PROCESSING:

Variable Identification – Microscope Magnification:

Table 1
Table title

Experimental Measurement
Description
Variables Units
Independent Objective lenses -
Dependent Magnification power x

Table description: Table 1 shows that the response variable of this experiment was the
magnification power which was produced by the different objective lenses.

Calculations:

Total magnification Power 4x

Eyepiece lens x Chosen objective lens = TMP

10x X 4x = TMP

40x = TMP

Total magnification Power 10x

Eyepiece lens x Chosen objective lens = TMP

10x X 10x = TMP

100x = TMP
Total magnification Power 40x

Eyepiece lens x Chosen objective lens = TMP

10x X 40x = TMP

400x = TMP

Total magnification Power 100x

Eyepiece lens x Chose objective lens = TMP

10x X 100x = TMP

1000x = TMP

Variable Identification – Size of the cell:

Table 2
Table title

Experimental Measurement
Description
Variables Units
Independent Type of cell -
Dependent Size of cell µm

Table description: Table 2 shows that the response variable of this experiment was the size of the
cell which was produced by the type of cell.

Calculations:

Estimated size for Bacterial cell

FOV1 x TMP1 = FOV2 x TMP2

1.2mm x 40 = FOV2 x 1000

1.2mm x 40 = FOV2
1000

0.048mm = x 1000µm = 48µm = FOV2

1mm

Real cell size = FOV2 .

# of cells

Real cell size = 0.048mm

4

Real cell size = 0.012mm

0.012mm x 1000µm = 12µm

1mm
Real cell size = 12µm
Estimated size for Plant cells

FOV1 x TMP1 = FOV2 x TMP2

1.2mm x 40 = FOV2 x 400

1.2mm x 40 = FOV2
400

0.12mm x 1000µm = 120µm = FOV2

1mm

Real cell size = FOV2 .

# of cells

Real cell size = 0.12mm

2

Real cell size = 0.06mm

0.06mm x 1000µm = 60µm

1mm

Real cell size = 60µm

Estimate size for Animal cell

FOV1 x TMP1 = FOV2 x TMP2

1.2mm x 40 = FOV2 x 1000

1.2mm x 40 = FOV2
1000

0.048mm x 1000µm = 48µm = FOV2

1mm

Real cell size = FOV2 .

# of cells

Real cell size = 0.048mm

5

Real cell size = 0.0096mm

0.0096mm x 1000µm = 9.6µm

1mm

Real cell size = 9.6µm

Data:

Table 3
Table title

Bacterial cell Plant cell Animal cell

10x estimated size 1200µm 6000µm 960µm
40x estimated size 4800µm 24000µm 3840µm

Table description: Table 3 shows the estimated size produced by the chosen objective lenses.
which is being studied on different types of cells?

Variable Identification:

Table 4
Table title

Description Bacterial cell Plant cell Animal cell

Image

Shape Bacilus Rectangular Cocus

Sizes Light microspe Light microscope Light microscope
Number of cells Unicellular Multicellular Multicellular
Nutrition Heterotroph Autotroph Heterotroph
Membrane bound Prokaryotic Eukaryotic Eukaryotic
structures
Kingdom or Domain Bacteria Plant Animal
Grade of complexity Mild Mild Mild

Table description: Table description: Table 4 shows the criteria’s in which a cell can be divided
onto and the different classification of these 3 types of cell.

8. DISCUSSION:

The cell size depends on the type of cell, and each one of the cells has different properties
and parts. Most of the cells can be easily seen with the 100x objective lens, but you need
the immersion oil to use that objective lens.

9. CONCLUSIONS:

What we can conclude from this experiment is that the cell size can vary from their type, this
meaning that the cell will vary in every other aspect too, most of the time they do.
10. RECOMMENDATIONS:

11. REFERENCES: utilice normas APA

How to Mount Microscope Slides. (n.d.). Microscope.com.

https://www.microscope.com/education-center/how-to-guides/mount-slides/

Microscopes. (n.d.). https://education.nationalgeographic.org/resource/microscopes/

Study Mind. (2023, February 13). ᐉ How to Calculate the Actual Size of Cell? Formula and

Examples. https://studymind.co.uk/notes/calculating-cell-size/

ANNEXES: Virtual Lab Screenshots