Capillary

chromatography

INTRODUCTION

CEC is a combination of HPLC

and capillary electrophoresis.
Separation is achieved by
electrophoretic migration of
solutes and differential
partitioning.

PRINCIPLE

Electroosmosis is the motion

of liquid induced by an
applied potential across a
porous material, capillary
tube, membrane or any
other fluid conduit.
Electrophoresis is the
solute's response to applied
electrical field in which
cations move towards
cathode, anions move
towards anode and neutral
species stay stationary.
INSTRUMENTATION

The capillary is packed with

stationary phase. To
introduce the sample, the
capillary inlet is placed into a
vial containing the sample
and then returned to the
source vial.
The migration of the
analytes is then initiated by
an electric field that is
applied between the source
and destination vials.
The analytes separate as APPLICATIONS
they migrate due to their
electrophoretic mobility and
Analysis of pharmaceuticals
are detected near the outlet
compounds, biological and
of the capillary.
food components and
The output of the detector is
agricultural compounds.
sent to a computer.
Analysis of biomolecules like
The data is then displayed as
amino acids, enzymes,
an electropherogram, which
protein separation etc.
reports detector response as
a function of time.

REFERENCES
(n.d.). Retrieved from https://www.youtube.com/watch?v=55M8l2-6fJY
(n.d.). Retrieved from
https://en.wikipedia.org/wiki/Capillary_electrochromatography#:~:text=%20Capillary%20electrochromatograph
y%20%28%20CEC%29%20is%20a%20chromatographic,analytical%20techniques%2C%20high-
performance%20liquid%20chromatography%20and%20capillary%20electrophoresis.