EXPERIMENT NO.

IDENTIFICATION OF AMINO ACIDS THROUGH COLOR REACTION TESTS

SUBMITTED BY:

Kristen Kyle H. Endrina

Course and Sec

BSN 1-C

SUBMITTED TO:

Ms. Velasco

Date

December
 I. Introduction

Amino Acids are the monomers of protein. They are the building blocks of it. We all know that
protein is one of the biomolecules needed by human beings. That is why the formation of these
amino acids is also important to know. There are about 300 amino acids but only 20 of them can be
found in the human body. Some are essential and needed by the human body in various amounts
and can be acquired through foods. Examples of these are Tryptophan, Isoleucine, leucine,
Threonine, Methionine, Phenylalanine, Lysine, and Valine. Arginine and Histidine are samples of
Partially Essential. Some are also non-essential and can be synthesized already by the human body.
Examples of these are Cysteine, Proline, Glutamic Acid, Glutamine, Aspartic Acid, Asparagine,
Tyrosine, Alanine, Serine, and Glycine. These qualitative tests will help in determining the presence
of the different amino acids.

LEARNING OBJECTIVE

At the end of the experiment, the students should be able to:

1. Identify the specific amino acids content of the protein samples

MATERIALS:

Equipment and Glasswares:

12 test tubes, test tube rack, test tube holder, test tube brush, medicine droppers, water bath, 1

50 ml graduated cylinder.

Reagents:

Protein samples (5% egg albumin, casein, gelatin, and peptone), 40 % NaOH, lead acetate

solution, bromine water, α naphthol solution, ninhydrin solution, H 2 SO 4, Sodium nitrite solution, 1,2-

naphthoquinone-4-sulfonate, 10% NaOH, Sodium Dithionite (Na 2 S 2 O 4), Sodium nitroprusside, conc.

HNO 3, dilute ammonium hydroxide solution, glyoxylic acid, conc H 2 SO 4.