Certified

Certified Refer
Reference
rence
Materials
for
UV,
U Visible,
V, V NIR
isible, N and
IR and IIR
R
Molecular
M Spectroscopy
olecular S pectroscopy

RM-06HLKCTX/USP/MT
RM-06
6HL
LKC
CTX/USP/M
MT
Set Serial No: 250
25002
002

Customer Detai
Details:
aills:
ai
TEC
T
TE C Instrume
Instrumental
ent
ntal S.A,,
B
Bl
Blas
as Parer
Parera
era 1007,,
er
Florida,
Floridda,
a, B
Buenos Aires
Argentina
g

The customer information stated on this page,

number 1 of 3 applies to all certificates.

UKAS accreditation applies to all

Wavelength,
Transmission/Absorbance, Stray
Light references, and those used for
Resolution measurements.
0659 4001
 Reference Material Certificate of Calibration and Traceability

Potassium Dichromate in Perchloric acid sealed in Far UV quartz cells

Starna Scientific Ltd for use as a linearity and photometric accuracy reference in the UV.
52/54 Fowler Rd
HAINAULT Certificate Number: 110468
Essex IG6 3UT Certificate
Certific
icat
atee Da
Date
Date:
te:: 09 September 2021
England Expirati
Expiration
Expi tion
ti on D
Date: 10 September 2023
Analysis
A nal
aly
al Number:
ysis Numumbe
umb r:
be MT09092101
email: starna@starna.com Sett Serial Numbe
Se Number:
ber:
be r
r: 25002
Cell Serial Number
Number:
er:: 66166
Page Number 2 of 3 Blank Serial Number: 68591
68

Description of Referen
Reference
nce M
Material:
aterial:
Potassium Dichromate crystal
crystals,
als,
s, SRM
RM 935a, are purchased from NIST and solutions aare re p
pre
prepared
r pared
re d ac
according to the
procedure supplied with th thee ccrystals.
rys
ysta
ystals. The solutions are sealed by heat fusion in high
ta quality
gh q
quali
lity
ty FFar UV V qu
quartz cells.
The sealed solutions are then
re tthe
h n anaanalyzed performance
nalyzed and certified for use in assessing the perfor
orm
ormance o off spe
spectrophotometers
pect
pe c rophot
ct otom
ometers
region
in the Ultraviolet reg
egio
ion in aaccordance with the instructions that are issued with th N
NIST SRM M 93935a.
935a
5a.
5a

Certified
dVValues
allues of Reference Material:
The Potass
Potassium
ssiu
ium Di
Dichro
Dichromate
roma
romate
ma te ffil
filled
ille
il led
le d ce
cell
cells
llss ar
ll aree me
meas
measured
asur
asured aaga
ured
ur against
gain
ga inst
in st aan
n air re
refe
reference.
fere
fe renc
re ncee.
nc e. ThThee nenett ab
abso
absorbance
sorb
sorban
rb ance
an ce v
val
values
alue
al u s
listed
are list
sted
ed in th
the table be
be
below. procedures
Under the analytical procedure used,
res us
re used
ed, as outlined by NIST in the Appen
ed Appendix
end
en NIST
dix NI
N IS
ST
Special
Spec
Sp ecia Publications
ial Pu
Pubblications 2
bl 260-54.
260
60-5
60-5
60-54.
4. TThe combined
he ccom
ommbib ned aanalytical
d annal
alyt
al ytic
ytical
ic al aand instrumental
nd iin
nstr
tru
umen
umen
um entatall un
ta uncertainties
unce
cert
ce rtai
rtaint
ai ntie
nt iess at tthe
ie he 9
95% confidence
5% ccon
onfi
on fi nc
fi
fidenc level
nce leve (k=2)
vell (k
(k=2
=2)
=2)
=2
0.0037
is 0
0.003037
03 7 A at 20 mg/l, 0.0045 A at 40 0 mg/l, l, 0.0049 A at 60 mg/l,
0 mg g/l 0.0058
/l, 0.
0.00
0058
00 58 A aatt 80 m mg/l,
mg/g/l, aand
g/l,
g/ 0.0068
nd 0
0.0
.006
.0 8 A aatt 1
068
06 100
10 mg/l.
00 mgg/l.
g/

The reported expanded uncertainty is b

Th baseded
do standard
on the st
staandard unc
an uncertainty
ncertain
nc inty
in multiplied
ty m
mul
ulti
ul tipl
ti plie
plied
ie coverage
d by a cco
over
erag
ag fa
age
ag factor
fact
ctor
ct
k = 2, providing a level of confidence
ce o approximately
of apppr
proximat
pr ateel
at el 95%. T
ely The
he uncertainty evaluation has been car carried
arried
ar ed o
out
in a requirements.
accordance with UKAS requiremen entts.
en ts

Nominal
Nomi
mina Concentration:
n l Co
C ncentration:
Wavelength:
Waave
W ave
vele
vele
leng
leng
ngth
th::
th Absorbance
Ab
A bso
sorb
r an
rbance
ance
ce Normal
N
Noorm
rmal
al Cert.
C t.
Potassium
Pota
Po tass
s ium Di
Dich
Dichromate
chro
chromate B
ro Bl
Blank
lank
lan
lank
350 nm 0.0319
319
iall No: 68
Cell Seria
Serial 6859
591
59
685911 313 nm 0.0332
332
257 nm 399
0.0399
235
35 nm
n 0.0437
0.0437
Potassium Dichroma
Dichromate
mate
te 6
600 mg
mg/l
/l
350 nm 0.6661
0. 661
0.6 61 0.6342
Cell Serial No: 66166 313 nm 0.3218
0.32
218 0.2885
257 nm 0.8935
35 0.8536
235 nm 0.7831
0.78
0.7831 0.7394
 Page Number 3 of 3

Set Serial Number: 25002 UKAS Accredited Calibration Laboratory No.

Certificate Number: 110468 0659
Calibration Date: 09 September 2021
Analysis Date:: 09 September 2021

Certifying Instrument Qualification: Duration of Certificate:

All calibration is performed on one of a series of reference This certification is valid for a maximum period of two
spectrophotometers. The instrument is tested and confirmed
confirirme
med d to years from the date of issue or sooner if specified by
perform to the manufacturers written specification ove
over
verr thee thee user's own protocols.
th
analytical range used for the reference material certification.
cer
erti
tifi
fica
cati
ca tion.
ti
The following references and procedures are usused
ed in
n th
the Re-certification
Re-ce
ertification Procedure:
quality performance procedures relating to tthe
he q
qua
qualification
ualification of
ua
the spectrophotometer performance: All re
refe
reference
ference ma
fe materials are originally certified and
supplied ed iin a useabl
ble condition. There is no warranty
useable
Absorbance: NIST SRM 930e & 19
1930, Do
Double Aperture method. for fitness be beyo
beyond
yond ini
yo initial
niti
tial receipt by the customer.
Wavelength: NIST SRM 2034,
203
034,
4, E
Emiss
Emission
ssio
ss ion lines of mercury & deuterium.
io When refer
references
ereence
er cess n
ne
need
eed to o be re-certified or inspected
Stray Light: NIST SRM 2
203
2032,
032, K
KC
KCl,
Cl, KI & lithium carbonate.
Cl for any y re
reason,, cust
customers
stom
st om sh
omers
om shou
should
o ld return them to the
Resolution: Benzene
Benzen
enee va
vapour
vapour,
ur, half width of deuterium 656.1 nm line.
ur Starna
Star
arna
n ISO/IEC
na EC 170
17025
702525 & 17034 34 accredited calibration
labo
lab ratory, where
bo
laboratory, wher
ere al
all prevevio
ev ious dat
io
previous ataa are collated.
data
Calibration
nMMethod:
ethod: On receipt by St Starna
na Scientifi ficc the re
fi
Scientific refe
ferences are
references
measured
meas
me asur
as ured
ur ed ""As
As RRec
Received"
ecei
ec eive
ei ved"
ve d" bbefor
before
oree cl
or clea
cleaning
eani
ea
ani
ning
ng u
und
under
nder
nd er tthe
he
The conditio
conditions
ions
ns ooff aanalysis
nalysis u
used to g
generate the certified re-certification p procedure
procedure. re. "A
re "As
As received" data are
As
values o
onn th
this
is ccertificate are as listed in th
the ch
cha
chart
ar below:
art
ar available
avai
av aila
ai labl
lablee on rreq
bl request.
eque
eq uest
ue st..
st

Material:
Mate
teri
rial:: Sealed Potassium D
Dic
Dichromate
ichrom
ic omate cell
om ll
Storage
S torage aand
nd C
Care:
aree:
Refe
Reference:
ference:
e: 68591
Scale:
Scal
ale: Absorbance Refe
Re
Reference
ference (s) sh shou
should
ouldld always be stored in tthe he box
Range:
Rang
nge:
e: 235 nm tto
o 35
3500 nm
nm prov
pr
provided
ovid
ov ided
id ed and
nd h han
handled
andl
an dled
dl ed wwit
with
ith
it h ex
extr
extreme
trem
tr emee ca
em care
care.
re. Re
re Refere
References
renc
rences
nc es aare
re
Slit wid
width:
idth
th: 1.0 nm frag
fr
fragile
agil
ag ilee an
il and sh
and shou
should
ould b
ould
ou bee in
inse
inserted
sert
se rted and
rt nd rrem
removed
emov
em oved fro
ov from
rom
ro m th
thee
Temperature:
Temperat atur
ure:
e: 23.5 +
23 +/-
/- 0
0.5 ºC inst
in
instrument
stru
st rume
ru ment
me nt tak
taking
akin
ak ing
in g ca
carere n
not
ot tto twtwis
twist
istt or apply llev
is leverage
everage
agai
ag ainst the cell
against ll hhol
oldder, as th
holder, thisis m
ma
mayay crack tthehe reference.
Instructions
Instruction
ns for U
Use:
s e: Dama
Da m ge in the form
ma
Damage rm of scscraratc
ra tch
tc
scratches he or con
hes ontamination may
contamination
alte
al terr th
alter the certififie
ied
certified d va
valuluees
lu
values es ssig
ignificant
ig
significantly ntly
ly ssuc
uch th
such that
at they y
Determine the Absorbance
Absorbrban
ance off ea each
ch ccel
cell
elll ag
agai
against
ains
nstt th
thee su
supp
supplied
ppli
lied
ed needed rre-
re-certifying
e-ce
certif
ce ifyi
yingng oor eveve
even
en com
en complete
mpl
pletete re
repl
replacement.
placemenent.
t. F
For
blank at each of the four li
list
listed
sted wavel
wavelengths.
elen
el engths. Repeat several
en cleaning see ee tthe
he g gui
guidance
uida
uid nce no
da note
notes
tes at the back of this
times. To assess photometric ic aacc
accuracy,
ccuracy, y, ccom
compare
ompare the net
om booklet.
Absorbance reading at each concentration
conce centration on aand
n wavelength to
nd
the published value on the certifica
certificate.
cate
te.. The ababso
absolute
solu
so lute
lu Cali
Ca
Calibration
lib
li bratio
ion
ion performe
performed
med by:
difference between the mean measured ed v value and nd tthe
h certified
he
value will not exceed the sum of the certi certified
tifi
fied
ed uncer
uncertainty
erta
ertain
ta inty
in
and the specified accuracy of the instrument,
instrument nt,, if the ins
instrument
nstr
trum
tr ument
um
is performing correctly. S. P.
P Beaird
Calibration
Ca Technician
Instrument Dependencies: Approved Signatory:
Ap

Th instrument
The inst nt mustt be ddesigned
ig d to bbe used
ed iin th
the ultr
ultraviolet
iolet
region down to 230 nm and have a spectral bandpass of 1.6 nm
or less. Consult your instrument owners manual for this A. Wakelin CSci CChem MRSC
information. Calibration Manager

All rights reserved. This certificate may not be reproduced other than in full, except with the prior written approval of the issuing
laboratory. This certification must not be used by a third party to claim product endorsement by the accreditation body or any agency
of the U.S. or U.K. governments
 Reference Material Certificate of Calibration
and Traceability

Starna Scientific Ltd Holmium oxide in perchloric acid sealed in a quartz cell
52/54 Fowler Rd for use as a wavelength accuracy reference in the UV
HAINAULT and visible spectrum.
Essex IG6 3UT
England
g Set Serial Number: 25002
Tel. +44 (0)20 8500 1264 Cell Serial Number: 66696
Number:
Certificate Nu
Numb
mber
mb er::
er 110469
email: starna@starna.com Certific
icat
ate Da
Certificate Date
te:
te
Date: 09 September 2021
Expiration
Expi
Expira
ration
on D
Date: 10 S
Sep
September
eptember 2023
Analys
An y is number:
ys
Analysis MT090909
092101
MT09092101
Page Number 2 of 3

Description of Reference
Refereence M
Material:
aterial:
This reference material consists
consnsisists
ts off an aqueous solution of 4% holmium oxide in 1 10%
0% per
perchloric
erch
chloric ac
ch acid
id which is
permanently sealed by heat at ffusio
fusion
ion in a high quality far UV quartz cell. The refe
io reference
fere
ference ma
re materi
material
rial
al iis desi
designed
sign
gned for the
verification and calibra
calibration
rati
tion
on ooff the wavelength scales of visible and ultraviol
ultraviolet
olet
ol et spectroph
spectrophotometers
photom
ometer erss having
er ng n
nominal
spectral bandwidths
bandwidththss of 3 nnmm or less.

Certified
dVValues
alues ooff Reference Material:
The holmium
holm
lmiuium ox
oxide filled cel
cell
elll is measure
el measured
red
red in tthe
he abs
absorbance
bsorba
bs bance mo
ba mode de aga
against
gain
inst
in st aan ai
air bl
blank,
k, ove
over
ver th
the wave
wavelength
veleng
ve ngth
ngth rran
range
an
ange
an
of 660
60 tto
o 23
2300 nm. For each spectral band
bandwidth,
ndw
nd width,
h, a baseline co
correc
correction
ection is performed with an empty cell
ec cell h
holde
holder.
der.
de
The 14 m ma
maximum
aximum absorption peaks are id identifi
identified
fied and ccer
fi certified
ertified tto
er o be w
wit
within
ithi
it hin
hin th
thee ex
expe
expected
pect
pe cted
ct ed w
wav
wavelength
avel
avelen
el eng
en gt ra
gt
gth ra e to
range tolerancee
for ea
each
ch spectral bandwidth (SBW), as sp specifie
specified
ied by tthe
ie he NIST re
he refe
reference
ferenc
fe ncee co
nc cont
control.
ntro
nt rol.
ro l.

The combined
combin
ined
in ed analytical and instrument uncertainties at th
thee 95%
% conf
confidence
nfid
nfiden
idence
en ce llevel
el iis 0.
0.11
11 n
nm
nm.
m.

The reported
repo expanded
ported exp anded uncertainty
xpan
xpan unce
cert
cer
ce rta
rta
aiint
int
nty is based
is b
bas
ased o
as
ased onn th standard
the st
sta
anda
dard
da
ard uncertainty
rd u
unc
nceer
nc ertta
ain
ain
inty m multiplied
mu ulti
ullti
tipl
plie
pl ied
ied by coverage
by a ccov
over
ov erag
er ffactor
agee fa
ag act
act
ctor
or
k = 2, providing
pro
rovi
viding a llev
level
evel
ev e off co
el cconfidence
conf
onf
nfidence
f ce ooff ap
aapproximately
appr
ppr
prox
oximat
oxx atatel
el 95
ely
el 95%
95%.
5%. The
he u
unc
uncertainty
ncer
nc eerrta
tain
inty
inty eeva
evaluation
valu
va luat
luat
luatio
ion
io n ha
hass be
been
een
en ccarri
carried
rieed
ri ed o
ou
out
ut
in accordanc
accordance
ncee with U
UKAS
KA AS requirements.

SBW Wavelengths
Wavelength
thss in nan
nanometers
anom
anometer
om erss of p
pea
peak
eakk ma
m
maxima
xima
ma as re
referenc
referenced
nced
ed tto
o ai
air,
r, +
+/- 0.11
1 nm

0.10 640.41 536.42 485.

48
485.20
5 20 467.
46
467.78
7.78
7. 78 452.02 416.02 385.36 361.27 345.46
46 3
333.48
33.
3.448
48 287.03
03 278.15 249.78 240.97

0.25 640.41 536.43 485.21 467.79

46 451.
45
451.98
1.98
1. 416.04 385.39 361.27
27 345.45
45 333.47
47 287.04 278.15 249.79 240.98

0.50 640.43 536.45 485.21 467.80 451.91 416.07

41 385.45 361.27
27 345.43 333.47 287.08 278.15 249.81 241.02

1.00 640.50 536.56 485.23 467.82 451.45

45 416.25 385.
38
385.61
5.61
5. 61 361.25 345.38
34 333.48 287.22 278.13 249.89 241.12

1.50 640.62 536.71 485.26 467.86 451.33 416.

416.42
6.42
42 385.
38
385.70
5.70
70 361.18
36 345.38 333.49 287.40 278.11 249.98 241.13

2.00 640.79 536.86 485.25 467.90 451.32 416.57 385.80 361.12 345.42 333.47 287.52 278.10 250.03 241.12

3.00 641.15 537.21 485.21 468.11 451.36 416.89 386.00 361.11 345.53 333.47 287.57 278.05 250.07 241.04

This certification is issued in accordance with the laboratory accreditation requirements of the United Kingdom Accreditation Service
It provides traceability of measurement to recognized national standards, and to units of measurement realised at the National
Physical Laboratory, or other recognized standards laboratories.
 Page Number 3 of 3

Cell Serial Number: 66696 UKAS Accredited Calibration Laboratory No.

Certificate Number: 110469 0659
Calibration Date: 09 September 2021
Analysis number: MT09092101

Certifying Instrument Qualification: Duration of Certificate:

All calibration is performed on one of a series of reference This certification is valid for a maximum period of two
spectrophotometers. The instrument is tested and confirmed to years from the date of issue or sooner if specified by
perform to the manufacturers written specification over th thee the user's own protocols.
analytical range used for the reference material certification.
certifi
fica
cati
tion
on..
on
The following references and procedures are used
ed iin
n thee Re-certification
Re
e-certification Procedure:
quality performance procedures relating to the
he q
qua
qualification
uali
lifi
li fication of
fi
the spectrophotometer performance: A
Al
All
lll referenc
reference ncee materials are originally certified and
supp
ppli
pp
suppliedlied in a us
li usea
e ble condition. There is no warranty
useable
Absorbance: NIST SRM 930e & 1930
1930,
30,, Do
Doub
Double
uble
uble Aperture method. for fitn
fitness
tnes
tn ess beyond
es nd iinitial receipt by the customer.
Wavelength: NIST SRM 2034, Emission
Emi
miss
ssion
n li
lines of mercury & deuterium. When ref references
efer
ef eren
er ences need
en ed to be re-certified or inspected
Stray Light: NIST SRM 2032,
2, K
KCl, KI & lithium carbonate. for any reas reason,
aso
as on, cu
customer
customerserss should return them to the
Resolution: Benzene vapour,
vapo
pour
ur, ha
half
lf w
width of deuterium 656.1 nm line. Starna IISO ISO/IEC
SO/IEC
SO EC 170
17025
7025
70 2 &1 170
17034
7034 accredited calibration
labora rato
ra
laboratory,tory, wher ere al
where all pr
previous us d
data are collated.
Calibration M
Method:
ethod: On rreceipt by St Starna
na Scicien
entific th
en
Scientific thee references are
measured "As R
me Rec
Received"
ecei
eived" befbefore
efor
efore
or
r clea
cleaning
eani
ning under the
The conditionsns ooff anal
analysis
alysis u
al use
used
sed
se d to g
gen
generate
ener
en erat
eratee th
at thee ce
cert
certified
rtif
rtif
rt ifie
ied
ie d re-certification
re-c
re-cer
-c erti
er tifi
ti fica
fi cati
ca tion
ti on p
pro
procedure.
rocce
ro cedu
cedu
dure
dure
re. "AsAs rrec
received"
ecei
ec eive
eived"
ved" d
d" dat
data
ataa ar
at aree
values on th
this
is ccerti
certificate
tifi
ti ficate are
fi re as listed in the chart below: available on request.

Cell P
Pat
Pathlength:
athl
hlengt
gth:
gt h:
h: 10 +/- 0.01 mm
Cell M
Mater
Material:
eria
erial:
ia Spectrosil Quartz Storage
S torage aand
nd C
Care:
aree:
Reference:
Refe
fere
renc
ren e: Air
Reference (s) should alw lway
lw
alwaysays be ssto
ay tored in the box
stored
Scal
Scale:
ale: Absorbance prov
pr
provided
ovidided
id ed and nd hhan
handled
andl
dled
dl ed wit
with
ith
it h extreme care care.
re.. F
re Fi
Filters
ilt
lters aree
Range:
Rang
nge: 660 nm tto o 23
2300 nm
nm frag
fr
fragile
agil
ag ilee an
il aandd sh
shou
should
ould
ou ld bbee in
inse
inserted
sert
se rted
rt ed aand
nd rrem
removed
emo
em oved from
oved m th
the
Slit wid
width:
idth
th: Multip
Multiple
iple
ip le inst
in
instrument
stru
st rume
ru ment
me ent
nt ttak
taking
akin
akin
ak ingg ca
care
re nnot
ot tto tw
ot twis
twist
istt or
is or aapp
apply
pply
pp ly llev
leverage
ever
ev erag
eragee
ag
Temperature:
Temperat atur
ure:
e 23.5 +
23 +/-
/- 0
0.5 ºC agai
ag
against
ains
ai nst th
nst
ns thee ce
cell
ll hhol
holder,
olde
ol d r, aass th
de this m mayay ccra
crack
rack
ra ck thehe ffil
filter.
ilter.
il
Dam
Da mage in the form
Damage rm of scratc tch
tc
scratcheshes
hees or contami mination may
contamination
alte
al terr the certifie
te
alter ied va
certified values ssig igni
ig nifi
ni ficantly ssuc
fi
significantly uch that they
such
Instructionss ffor
or U
Use:
s e: need
ne ed rre-
e certif ifyi
re-certifyingying
ng oorr ev
even
en ccompletee re replplacememen
replacement. e t. FFor
or
clea
cl eani
ning
cleaning ng ssee tthehe g gui
uida
uidanc
danc
da
guidancencee notess at the b back of tthi hiss
this
Carefully insert the holmium
holmi
mium
um fille
filled
led
led ce
cell
ll iinto the cell holde
holder
der of booklet. t.
your instrument, touching on only
ly the fro
frosted
rost
sted
st eed
d sides or by holding
the top of the cell. The cell is ffra
fragile
ragi
gile and
nd ssho
should
hould always be
ho
handled with care. Leave the refer
reference
eren
ence cell ho hold
holder
lder empty as
ld Cali
Calibration
libr
li bratio
br ion
n performe
performed
medd by:
all measurements are to be made against
agai
ains
nst air. In th thee
Absornbance mode, scan to find the peak k ma
m
maxima
xima aandnd then
nd
compare them to the certified wavelengths on
this certificate as indicated for the spectral ban bandwidth
andw
dwidth (SB
(SBW)
SBW)
SB W)
W)
used by your instrument. If you find any significant nt
differences, it is recommended that an approved S. P. Beaird
manufacturer's representative inspects the instrument to Calibration Technician
determine the source of the difference. Approved Signatory:

Instrument Dependencies:
The instrument to be tested should have a SBW not
exceeding 3 nanometers. Consult the instrument owners
handbook for this information. A. Wakelin CSci CChem MRSC
Calibration Manager
All rights reserved. This certificate may not be reproduced other than in full, except with the prior written approval of the issuing
laboratory. This certification must not be used by a third party to claim product endorsement by the accreditation body or any agency
of the U.S. or U.K. governments
 Reference Material Certificate of Analysis

Calibration Lab. Potassium chloride aqueous solutions, sealed

Starna Scientific Ltd in far UV quartz cells, for use as a stray light
52/54 Fowler Rd reference in the ultraviolet spectrum.
HAINAULT
Essex IG6 3UT Certificate
Certif
Ce ific
if icate Nu
ic Numb
Number:
m er: 110470
England Certificate
Cert
Ce rtificate Date:
rt 09 September 2021
Tel. +44 (0)20 8501 5550 Expiration
E xpiration Date: 10 September 2023
Analysis Number: MT09092101
email: starna@starna.com Set Serial Number: 25002
25
'10 mm Cell' Serial Number: 65145
6514
65145
Page Number 2 of 3 '5 mm Cell' Serial Number: 65133
6513
1333

Description of R
Reference
eferrence Material:
This reference consists
con
onsi
sistss of two high quality far UV quartz cells. Both cells cco contain
on
ontain a 12 g/ll aque
aqueous
ueous so solu
solution
lution off po
lu potassium chloride,
where the 'Sample'
'Sam
amplp e' ccel
cell
ell is 10 mm, and the 'Reference' cell is 5 mm path le
el leng
length.
ngthth. Both cells aare p permanent
permanently ntly
nt ly sealedd by heat fusion.
Used in th this
is fforma
format,
mat, the
ma he eess
essential
ssen
ssenti
en tial
ti al sspe
spectral
pect
pe ctra
ct rall ch
ra char
characteristic
arac
aracte
ac teri
te rist
ri stic
stic o
off th
the solution on iiss th
that
at iitt pr
prod
produces
oduc
od uces
uc es a ppea
peak
eak of k
ea
eak kno
known
nown A
no Abs
Absorbance
bsor
sorba
bancncee value. *
The memeas
measured
asured
ed wwavelen
wavelength
ength positions this peak at the maxim
en maximum
imu um A
um Abs
Absorbance
bso
bs orbance value. The reference mater material
erial is d
er designed for the
detection
detetect
ctio
ion of sstray lightht aatt wa
wave
wavelengths
vele
ve leng
le ngth
ng thss below
th w th
thee me
meas
measured
asur
asured
ur ed v value
value.
ue.. AS
ue ASTM TM E E38
E387-04(2014)
387-
38 7-04
7- 04(2
04 (201
(2 014)
01 4) g
giv
gives
ives
iv es tthi
this
hiss wa
hi wavele
wavelength
length ran
le range
ange
an ge aass 17
ge 175
5 to 2
200
00 n
nm.
m.
A cocomm
commonly
mmmonly used wavelength by inst instrument
strru
st ru nt manufacturers
rument manufacturers, rs,, et
rs etc.
c. ffor
or tthi
or this
hiss re
refe
reference
fere
ferenc
re ncee is 1
nc 198
98 n
nm.
m. A Allll p
pro
procedures
roce
ro cedu
ce
cedu
durres
re ar
aree im
impl
implemented
plem
pl e ented d in
aaccordance
accord
co rdance with ISO/IEC 17025 and IS
rd ISO Gu Guide 34. Addi Additional
diti
di tional
ti al iinformation can be found on the Starn Starna
rna we
rn web sitete aat www.
www.starna.com.
w.
w.starna.c
w. .com
om.

Measured
Measured Values of Refe
Reference
erencce Ma
Material:
aterial:
The po
pota
potassium
tassium chloride filled cell is scanned in Absorbanc
ta Absorbance ncee mo
nc mode over thee ra ran
range
nge 210 to 1 190
90 nnm,
m using the '5 mm' blan blank
ank
an k as the
reference.
referenc
nce.
nce. T
The Absorba
Absorbance
banc
ba nce pe
nc
nce peak
ak iiss ve
veri
verified
rifi
rified
fi ed aand
nd ccer
certified
erti
ertifi
ti fied
fi ed tto
o be ccor
correct,
orre
orrect
re ct,, in val
ct validating
alid
al idat
id atin
at ing thee pu
in puri
purity
rity
ri ty o
off p
pot
potassium
otas
otassi
assi
as sium
um cchl
chloride
hlor
hlor
hlorid
idee an
id andd it
its use
use as
a an
a
indicator of iins
in instrumental
ns
ns
nstrumen
enta
en tall st
ta stray li
ligh
light.
ght.
ght.
gh
`
The co
comb
combined
mbined anaanalytical
naly
nalyti
lytica
ti c l and instrumental uncertainties at the 95% confidence
ce llev
level
e el iis 0.11 nm.
The reported
report
rted expanded
ed expande
ded
de d un
un
uncertainty multiplied
is based on the standard uncertainty multiplie
ied
ied by a coverage fa
factor providing
or k=2, pr
prov
rov
ovid
i in
ing
g a coverage
ge
probability of approximately
approxima
a ate
tely 95%.
ly 9 5%. The uncertainty evaluation has been carried out in a with
accordance w ith U
UKASS rrequirements.
equ
quir
irements.
ir

Peak Wavelen
Wavelength
engt
gth (n
(nm)
(nm)*
m)*
m) * Measur
Measured
Meas ured
ed P
Pea
Peak
eak
k St
Stray Light
Ligh
Lightt Stray Light
St Ligh
Lightt
+/- 0.11 nm Absorbance
A
Absorbance value (A) Absorbance value
ue ((S)
S) Transm
Transmission
smission (T/%)
199.9 greater
grea
greate
eater than 0.70 A
te greater than 2
2.0
2.00
.00 A
.0 less
le than 1.0 T/%

IMPORTANT
* nd tthe Absor
The Peak Wavelength (nm) and orba
orbanc
ban e value (A) will vvar
Absorbance ary sign
ar
vary gnif
gnifiicantly
if ic
significantly from
fr om one instrument to another.

* The measurement details and information

inform
rmat
ation shown
show
own
ow n ab
a
above
ove simp
simply
m ly ind
mp indicates
nd
dic
icates the p
pu
purity
urity and suitability for use of the stray light
reference, permanently heat-fused seal
sealed
aled
ed into the cu
cuve
cuvette.
vett
vette.
e.

* The value assignments shown above must N NOT

OT be used to verify eithe
herr the wavelength and/or the Transmittance/Absorbance
either
scale of the spectrophotometer under test.

* Irrespective of the actual wavelength observed, the Absorbance value measured at the peak will give an estimate of the
instrumental stray light in this area of the spectrum - see "Suggested Instructions for Use" on page 3.

* If this resultant peak within the measured range indicates an Absorbance of greater than 0.7 A then the instrument is
deemed to be compliant with General Chapter "Ultraviolet-Visible Spectroscopy" <857> , of the United States
 Page Number 3 of 3
'10 mm Cell' Serial Number: 65145
'5 mm Cell' Serial Number: 65133 





UKAS Accredited Calibration Laboratory No. 0659
Certificate Number: 110470 
Calibration Date: 09 September 2021

Certifying Instrument Qualification: Duration of Certificate:

All calibration is performed on one of a series of high This certificate is valid for a maximum period of two
performance reference spectrophotometers. The instruments are years from the date of issue or sooner if specified by
tested and qualified to the manufacturers' published specification the user's own protocols. Although the references are
over the analytical range used for the reference material covered by a Lifetime Guarantee, this is subject to
certification. The following primary references fundamental
fundam
amen
ental ccertain
ce rtain conditions, see Guidance Notes.
procedures, are used in the qualification of the refer
reference
eren
ence
spectrophotometers: Re-certification
Re-c
certification Procedure:
Absorbance: NIST SRM 930e & 1930, Double
le A
Apert
Aperture
rtur
rt u e method.
ur
Wavelength: NIST SRM 2034, Emission li
line
lines
nes of m
mercury & deuterium. Alll re
Al referencee ma m
materials
terials are certified and supplied in a
Stray Light: NIST SRM 2032, KCl, KI & lithi
lithium
hiu
hium carbonate. useable
usea
eabl
blee conditio
bl condition.
ion.
n. There is no warranty for fitness
Resolution: Benzene vapour, half
lf w
wid
width
idth o
off deuterium 656.1 nm line. beyondnd iini
initial
niitial receipt
n recei
eipt
pt by the customer.
When refer eren
er e ce
en
referencesces needed tto be re-certified or inspected
Measurement Method:
Meth
hod: for any y re
reason
reason,on, cu
cust
stomers
t
customers s should return them to the
Starna
na IISO/IEC EC 170
7025
70
1702525 & 170
25 7034 accredited
17034
The conditions of analysis
analalys
ysis
is u use
used
sed to generate the measured
se cali
ca
calibration
libration labo
li laboratory,
bora
rato
tory, where
wh al
wh alll original data is
values on this certifi
certificate
fica
cate aar
are
rree as listed in the chart below: collated.
co

Cell Path length:

leng
ngthth: 10 +/-
/- 0
0.01 mm On receipt by Starna Sci Scientific
cieenti
ci tifi
ti ficc the references are
fi
Cell Material:
Matereria
ial: Spectr
Spectrosil
tros
tr osil
os il Q
Qua
Quartz
uart
ua rtzz
rt m
me
meas
measured
eas
asur
ured
ur ed ''As
As RRec
Received',
ecei
eceive
ei ved'
ve d', befo
d' before
fore cle
fo cleaning
lean
leanin
an ing
in g un
unde
under
derr th
de thee
Reference:
Refere
renc
nce: 1.2 % KCl in a 5 mm m path
th length cell rece
re
recertification
cert
rtif
ific
if icat
atio
ion
n pr
proc
procedure.
oced
edur
ed ure.
ure. 'As
e. As RecReceived'
eceeived'
ec d' d
data is
Scale:
Scal
ale:
e: Absorbance avai
av
available
aila
ai labl
blee on rreq
request.
eque
equest
ue st..
st
Range:
Rang
nge:e: 210 nm to 190 nm
Band
nd w wid
width:
idth:
id 1.0 nm +/- 0.04 nm
Temp
Temperature:
mperaturure:
ure: 23.5 +/- 1.0 ºC Storage
S torage aand
nd C
Care:
are:
Peak
ak rread at: 199.9 ++/-
/- 0
0.1
0.11
.11
.11 nm
nm
Refe
Re fere
fe renc
rences
nces
nc
References es ssho
houl
houl
ho uld al
should alwa
ways
wa
always ys be st
ys stor
ored iin
ored
or
stored n the bo
boxx pr
provo id
ov ided
provided ed
Suggested
Sugges
sted In
Instructions
nstrucctions ffor
or U
Use:
s e: &h ha
and
ndle
led
le
handled d wi
with eext
with xtre
xt reme
re
extrememe ccare.e. Q
Qua
uart
ua rtz cells ar
rt
Quartz are fragil ile &
il
fragile
should be inserted
sh inseserted
ed and rem emov
em oved
ov
removed ed ffrom thee in
instrument by
Scan over the requ
required
quired wav
wavelength
avel
av elen
ele gth range in Absorbance hold
ho
holding
lding the ce
ld cell cap
cap,
ap, taki
taking
kin
ki ng ccare not to ttwist or apply
ng
in either 'Double Be
Beam
Beam'
am' or ''Si
'Single
Sing
Si ngle
ngle Bea
Beam'
eam'
m' m
mod
mode
odee as
a fol
follows:
ollo
lows:: leve
le
leverage
verarage
ra g aga
against
gaininst
st tthe
he cce
cell
ell ho
el holder, as tthi
this
hiss mayy cr
crack th thee
cell
ce lls.
s. D
cells. Damamag
am
Damage agee in tthe
he ffor
orm
or
formm of sscr
crat
atches
scratcheses mmay
ay aalt
lter
er tthe
alter he
Single Beam Mode: certified vavalulues
lu
values es ssig
igni
ig n ficantly
ni
significantly ly ssuch that they need
* With an empty sample hol
holder,
olde
der,
r, basel
baseline/zero
elin
ine/
ine/ze
e/z ro the instrument re-certifying
re-certifyfyin
fy ing an
in and
d may, ass w with
ith cracks, require complete
against air. repl
replacement.
plac
acemen
ac ent.t. F
For clean
cleaning
anin
ing see Guidance Notes.
* Sequentially scan both the '5 mm
mm'
m' an
and '10 mmmm'' references, and
save the data values.
* Mathematically subtract the '5 mm' dat
data,
ata,
a, ffrom th
thee co
corresponding Cali
Ca
Calibration
lib
li bration pe
performed by:
'10 mm' data.

Double Beam Mode:

* Place the '5 mm' in the Reference Beam, and the '10
0 mm
mm' in
the sample beam.
S. P. Beaird
In either mode, the resultant spectrum should contain a clearly Calibration Technician
defined peak, at an associated maximum Absorbance value. Approved Signatory:
This measured peak maximum Absorbance value will be
related to the stray light value (S) by the following equation:

S = 0.25*10-2A
Where A is the measured peak maximum Absorbance value. A. Wakelin CSci CChem MRSC
Calibration Manager
All rights reserved. This certificate may not be reproduced other than in full, except with the prior written approval of the issuing laboratory.
This certification must not be used by a third party to claim product endorsement by the accreditation body or any agency of the U.S. or
 Reference Material Certificate of Calibration

Starna Scientific Ltd Toluene in hexane and hexane blank sealed in a quartz cell for
52/54 Fowler Rd use as a spectral resolution reference in the ultraviolet spectrum.
HAINAULT
Essex IG6 3UT Certificate Number: 110471
England Certificate Da
Date
Date:
te: 09 September 2021
Tel. +44 (0)20 8501 5550 Expiration
Expirati
tion
on Date:
D e: 10 September 2023
Analysis
Anal
alys
ysis
is N
No:
o: MT09092101
MT09
MT 09092101
Email: starna@starna.com Set Serial
Set S ri
Se rial
al Number:
N 250022
Cell
Ce Serial
Seerial Number:
S 65690
Page Number 2 of 3 Blan
Bl
Blank
a k Serial Number:
an 65675
6567
656 5

Description of Referen
Reference
ence M
Material:

This reference materiall co consis

consists
ists
ists of two Far UV quartz cells, both of which have been
en ppermane
permanently
nently
ly ssea
sealed.
ealed.
ea
One cell is filled with
wit
ithh a 0.02
0.02%
02%
02 % v/v solution of toluene in hexane. The other cell is a bblank, filled
ed wwit
with
ith he
hexa
hexane
x ne onl
only.
nly.
y.
The reference mamate
material
terial iiss designed for the verification of spectral resolution.

Certified
Certif
ifie
ied
d Va
Valu
Values
alu
lues
es ooff Re
Refe
Reference
fere
ferenc
re ncee Ma
nc Mate
Material:
ate
teri
teri
rial
al::
al

The tolu
The toluene
luen
lu e e in hexane filled cell is mea
en measured
easu
ea sured ag
su ag
against the hexane
ne bbla
blank
lank
la nk aatt th
thee ma
maxi
maximum
ximu
xi mum
mu m ab
abso
absorbance
sorb
so rban
rb ance
ance cclo
closest
lose
lo sest
sest tto 26
26 nm and
268.7 nd tthe
minimum
m
mi nimu
ni mum absorbance closest to 267.0 nm
mu nm. UsUsin
Using
ing the resultss fr
in from tth
the
he analysis, a ratio of absorbance maxim
he maximum
imum
im um ddivid
divided
ided
id ed by
absorbance
abso
ab sorbance minimum is calculated for eea
so each
ac ba
ach
ac ban
bandwidth.
ndwidth.
The
T he combined analytical and instrument
instrumental
ntal unc
nt uncertainties
nceertainties
nc es at the 95%% co
cconfidence
nfididen
id ence
en ce llev
level
evel
ev el ((k=
(k=2)
k=2)
k=2) iiss 0.
0.00
0.0049
0049
0049 AA.
The rreported
Thhe re based
ported expanded uncertainty is bba
as oonn the standa
ased standard
dard
da uncertainty
rd uncer
erta
tain
tainty
in multiplied
ty m
multipl
plied by a coverag
pl coverage
age factor
ag
k = 2, pproviding a level of confidence of approximately 95%.
%. T
The uuncertainty evaluation
ty eeva
valuation has be
been
en ccarried out
in accordance
accororda
ordan e with UKAS
danc
da UKA requirements.
KASS re
KA requ
quir
quirem
ir ements
em ts..
ts
Nominal Co
No Conc
Concentration
ncen
nc e tratio
ion
io n 0.
00.02
0.0
.02 % vv/v
/v Wa
Wavelength:
ave
veleng
ngth
ng th::
th Absorbance
A
Abbso
sorb
rban
rban
rbance
c
ce SBW
SBW

Blank Serial No: 65

nk Ser 65675
6567
675
67 268.60 0.7730 1.00
267.00 0.3381
Cell Serial No: 656900
Ratio
Rati
Ratio 2.299
2.2

Spectral Resolution:
Resol
olut
ution:

The chart below gives the expected

expe
pect
cted range ffor
or tthe values of the ratio based on the sp
spe
spectral
ectral bba
bandwidth
an
andwidth aand
nd temperature of
measurement.
THIS CHART IS PROVIDED FOR INF
INFORMATION
NFORM
OR ATION
ON
N ON
ONL
ONLY
Y - IN ADDITION TO
O TH
THE CERT
CERTIFIED
ERTIFIED VAL
ERT VALUES SHOWN ABOVE

Spectral
al B
Bandwidth
andwi
widt
dth
Temperature 0.5 nm 1.0 nm 1.5 nm 2.0 nm 3.0 nm
of measurement +/- 0.1 nm +/- 0.
0.11 nm
n +/- 0.1 nm +/- 0.1 nm +/- 0.1 nm

20 +/- 1 ºC 2.4 - 2.5 2.0 - 2.1 1.6 - 1.7 1.3 - 1.4 1.0 - 1.1

25 +/- 1 ºC 2.3 - 2.4 1.9 - 2.0 1.6 - 1.7 1.3 - 1.4 1.0 - 1.1

30 +/- 1 ºC 2.1 - 2.2 1.8 - 1.9 1.5 - 1.6 1.3 - 1.4 1.0 - 1.1

This certification is issued in accordance with the laboratory accreditation requirements of the United Kingdom Accreditation Service. It provides traceability of
measurement to recognized national standards, and to units of measurement realised at the National Physical Laboratory or other recognised standards laboratories.
 Page Number 3 of 3

Set Serial Number: 25002 UKAS Accredited Calibration Laboratory No.

Certificate Number: 110471 0659
Calibration Date: 09 September 2021
Analysis Date:: 09 September 2021

Certifying Instrument Qualification: Duration of Certificate:

All calibration is performed on one of a series of reference This certification is valid for a maximum period of two
spectrophotometers. The instrument is tested and confirmed
confir irme
med d to yyears
ye ars from the date of issue or sooner if specified by
perform to the manufacturers written specification oveover
verr thee thee user's own protocols.
th
analytical range used for the reference material certification.
cer
erti
tifi
fica
cati
ca tion.
ti Altho
Although
houg
ugh the references are covered by a lifetime guarantee
The following references and procedures are usused
ed inn th
the this is su
subj
subject
bject to certain conditions, see guidance notes.
quality performance procedures relating to tthe
he qua
qualification
uali
ua lification of
li
the spectrophotometer performance: Re-certification
Re-certiffication Procedure:
Absorbance: NIST SRM 930e & 19
1930
1930,
30, Do
Doub
Double
oub
u le Aperture method. All refere
reference
renc
re ncce materi
n materials
rial
als are originally certified and
Wavelength: NIST SRM 2034,
2034
34,, Em
Emissi
Emission
sion
si on lines of mercury & deuterium. supplied in a us usea
useable
eable co
ea cond
condition.
ndition. There is no warranty
Stray Light: NIST SRM 203
2032,
032,
2, KCl
KCl,
Cl, KI & lithium carbonate.
Cl for fitnes
fitness
esss beyo
es beyond
yond
nd iini
initial
n tial rrec
receipt
eceipt by the customer.
Resolution: Benzene v
vap
vapour,
apour,
r, h
ha
half
alf width of deuterium 656.1 nm line.
al When en rreference
referencesces ne
need
ed tto be rre-
re-certified
e-certified or inspected
forr any
an reason,, cust stomer
customers erss should
er ld rreturn them to the
Validation Method:
Method: Star
St arna ISO/IEC
Starna EC 1707025 & 1
17025 170
70 acc
7034
70
17034 ccre
redited calibration
accredited
labo
labora
bo rato
ratory
to
laboratory, ry,, wh
ry wher
her
eree al
where alll pr
prev
evious d
ev
previous dat
ata ar
data aree co
collated.
The conditions
conditio
ions
ns of an
analysis uused to establish the range valuess
on this ce
cert
certificate
rtif
ificat
atee are as liste
at listed
ted
ted in tthe
he ccha
chart
hart
ha rt below
below:
ow::
ow On rrec
receipt
ecei
ec eipt
ei pt bbyy St
Star
Starna
arna
arna S Sci
Scientific
cieen
ci en fi
entifi
fic the re
refe
references
fere
fe
fere
renc
nces
nc es aare
re
measured
meas
me asur
ureded ""As
As RRec
Received"
eceieiveved"
d" b bef
before
efor
efor cl
ore
or cle
cleaning
eaning
ng u
under tthe
he
Cell P
Pat
Pathlength:
athl
hlen
hlength:
en 10 +/- 0.01 mm re-c
re
re-certification
-cer
-c erti
er tifi
ti fica
fi cati
cation
ti on p
pro
procedure.
roce
ro cedu
ce dure
du re. "A
re "As
As rec
received"
eceived" d
ec dat
data
ata are
at
Cell M
Mat
Material:
aterial:
at Spectrosil Quartz avai
av
available
aila
ai labl
lablee on
bl on rreq
request.
eque
equest
ue st..
st
Refe
Reference:
ferenc
nce:
nce
e: Hexane
Scale:
Scal
ale: Absorbance
Range:
Rang
nge: 265 nm to 270 nm Storage
S torag
ge an
and
nd Care:
Slit w
wid
width:
idth: 1.0 nm
m
Temperature:
Temperat atur
ure: 23 +
23.5
23 +/-
/- 1
1.0 ºC Refe
Re
Reference
fere
fe renc
ren e (s
nc (s)) sh
shou
should
ou
o uld aalw
always
lwaay
lw ay be ssto
ays stored
tore
to red
re d in the
he b
bo
box
ox
ox
prov
pr ovided and h
provided ha andl
dled with ex
handled extr
trem
tr eme care. Re
em
extreme References are
Instructions
Instructio
ons for U
Use:
se: frag
fr ag and shou
ag
agile
fragile ould b
should be inse
sert
se rteed
rt
inserteded aannd remov
and oved
removed ed from the
inst
stru
rument takin
ru
instrument takingingg ca
care
re n
no
ot tto
ot
not o twist or aapply leverage
Carefully insert the ttol
toluene
olue
uene
ne iin
n he
hexa
hexane
x ne ffil
xa filled
ille
ledd ce
cell
ll aand h
hexan
hexane
anee bl
blan
blank
ankk aagainst
aga
gain
inst
st tthe
h cel
cell
elll ho
hold
holder,
lder
lde , as tthis ma
er may y crac
crack
ackk the re
refe
reference.
fere
renc
nce.
e.
into the cell holder of yo
your
ur instr
instrument
trum
um
ment
ent to
en touchi
touching
hing o onl
only
nly th
the fr
frostedd DDamage
amag
am agee in tthehe ffor
form
orm
or m of scratch
scratches
ches
es o orr co
cont
contamination
ntam
aminatatio
ion
n may
sides, or by holding the top o off the cell
cell.
ll.. Ce
ll C
Cells
e are fragile and should
ells alter the ce cert
certified
rtif
rt ifie
if ied
ie d values
va sig
significantly
igni
nificantly such that they
always be handled with care. D Det
Determine
etermine tthe he absorbance of the need re-re-certifying
e-cce
e- certif
ifyi
ying or even
yi en complete replacement. For
toluene in hexane cell against the he h
hex
hexane
e ane bl blan
blank
ank
an k at each of the clea
cleaning
eani
ea ning see
ni ee g guidanc
guidancencee notes.
wavelengths. Calculate the ratio as on p page tw twoo of
of this certificate.

Instrument Dependencies:
Calibration
Calibrat
atio
ion pe
performed by
by:
The instrument to be tested should have a spectral b
ban
bandwidth
andwidth
th
not exceeding 2 nm. Consult your instrument's user m man
manual
anual
for this information.
S. P. Beaird Calibration Technician
igna
nato
tory
ry:
Approved Signatory:

A. Wakelin CSci CChem MRSC

Calibration Manager
All rights reserved. This certificate may not be reproduced other than in full, except with the prior written approval of the issuing
laboratory. This certification must not be used by a third party to claim product endorsement by the accreditation body or any agency
of the U.S. or U.K. governments