Selection Scheme II

5´ 3´ Discarded fDNA
A
A
Desired reaction B

BA Selection by affinity-
chromatography
A
h·

BA

Photocleavage

B A

A
5´ 3´
Amplification (PCR)
Primer extention with natural dNTP´s
with modified dNTP`s

Regular DNA
Mod. DNA (fDNA)
 Desired Reaction:
NH2

B N N A
O N N
O -O P O O
O-
HN NH
O O OH
H H O
H
N
S N R
H
O
O O
O P O 5´ fDNA 3´
NH2 H N
H O-
N O
O NO2

HN NH
H H O R O NO2
H H
N N
S N N O
H H H O-
O O N
O P O 5´ fDNA 3´
O O

BA