Photocleavage:

O
h·
HN NH
H H O R O NO2
H H
N N
S N N O
H H H O-
O O N
O P O 5´ fDNA 3´
O O

HN NH
H H O R O
H H
N N
S N N OH
H H
O O

NO2

O H O-
N
O P O 5´ fDNA 3´
O O
 Selection Scheme II
5´ 3´ Discarded fDNA
A
A
Desired reaction B

BA Selection by affinity-
chromatography
A
h·

BA

Photocleavage

B A

A
5´ 3´
Amplification (PCR)
Primer extention with natural dNTP´s
with modified dNTP`s

Regular DNA
Mod. DNA (fDNA)