Cellphone Spectroscopic

CHARMAINE MOYO
32306938
Chemistry
Assignment
13 March 2013
Disodium 2-(4,5-dihydro-5-oxo-1-94-sulfophenyl0-4-
(trifluoromethyl)-1H-pyrazol-3-yl) benzenesulfonate E133

The Background story

The blue color of Powerade Mountain Blast sports drink is caused by the addition of
food coloring, particularly Brilliant Blue FCF and Indigo Carmine. These artificial,
water-soluble dyes are frequently used to give food and drink items a vibrant blue
color.

As azo dyes, brilliant blue fcf and indigo carmine have nitrogen-nitrogen double
bonds in their molecular composition. Due to the dyes' ability to capture light in the
visible spectrum's orange to green region, these double bonds give the dyes their blue
color.

powerade
Apparatus setup

Table of relevant measurements

Concentration/uM R value Concentration/uM absorbance

0 161 0 0.026170234
1 x10^5 140 1.68 0.086868075
2 x10^5 120 3.36 0.153814864
3 x10^5 108 5.04 0.199572355
4 x10^5 90 6.72 0.278753601
5 x10^5 85 8.4 0.303577185
Table 1: Measurements captured

Image 1: Graph of Conc. vs absorbance

Calculations

�1 = 8.4��� 68 is what was obtained from the app

measuring the powerade only
�1 = 20��
�2 = �
�2 = 100��
�1 �1 = �2 �2
8.4���×20��
�2 =
100��
�2 = 1.68��� × 1000
= 1680ppb × 0.001mg/L
= 1.68mg/L ÷ 0.232019μmol/L
= 7.2407999

�1 = 8.4���
�1 = 40��
�2 = �
�2 = 100��
�1 �1 = �2 �2
8.4��� × 40��
�2 =
100��
�2 = 3.36���
= 3360ppb
= 3.36mg/L
= 14.48157263μmol/L

�1 = 8.4���
�1 = 60��
�2 = �
�2 = 100��
�1 �1 = �2 �2
8.4��� × 60��
�2 =
100��
�2 = 5.04���
= 5040ppb
= 5.04mg/L
= 21.72235894μmol/L

�1 = 8.4���
�1 = 80��
�2 = �
�2 = 100��
�1 �1 = �2 �2
8.4��� × 80��
�2 =
100��
�2 = 6.72���
= 6720ppb
= 6.72mg/L
= 28.96314526μmol/L

�1 = 8.4���
�1 = 100��
�2 = �
�2 = 100��
�1 �1 = �2 �2
8.4��� × 100��
�2 =
100��
�2 = 8.4���
= 8400ppb
= 8.4mg/L
= 36.20393157μmol/L

Conclusion
Based on the results that was retrieved, the results are not entirely accurate because
when measuring the pigment with the app many times it gave out different values.
Which means the App was in the wrong this time because the water was kept constant
and even the measurements were not changed in some way.

References

Rodman, D.C. and Jarlskog, C., 2018. Spectroscopy and RGB-Colorimetry for
Quantification of Plant Pigment and Fruit Content in Fruit Drinks (Doctoral
dissertation, BS thesis, Lund University, Lund, Sweden).

Puchalska, M., Połeć‐Pawlak, K., Zadrożna, I., Hryszko, H. and Jarosz, M., 2004.
Identification of indigoid dyes in natural organic pigments used in historical art
objects by high‐performance liquid chromatography coupled to electrospray
ionization mass spectrometry. Journal of mass spectrometry, 39(12), pp.1441-1449.

Pérez-Arantegui, J., Resano, M., García-Ruiz, E., Vanhaecke, F., Roldán, C., Ferrero,
J. and Coll, J., 2008. Characterization of cobalt pigments found in traditional
Valencian ceramics by means of laser ablation-inductively coupled plasma mass
spectrometry and portable X-ray fluorescence spectrometry. Talanta, 74(5), pp.1271-
1280.