Next Generation Axil Scientific Pte Ltd

41 Science Park Road, #01-22/23 The Gemini

Sequencing - Singapore Science Park II, Singapore 117610
T: +65 6775 7318
Bioinformatics Analysis F: +65 6775 7211
Request E: ngs@axilscientific.com

CUSTOMER INFORMATION

Name:

Institute/Company Name:

Principal Investigator/Supervisor:
Contact No.: (Office)
Email Address:
Delivery/Collection Address:
Quotation Number:

NGS ID (if available): NGS 14139

NGS PROJECT OUTLINE

Experimental Information
Application Type: For ITS amplicon sequencing project (region ITS1)
*For 16S rRNA/ITS/18S amplicon sequencing project, please complete tab '3. Amplicon Seq Analysis' if comparison studies is needed.

Raw Data Size: 100,000 reads

Species:

Reference Genome Available? Yes ✘ No

To identify diversity of fungal community in soil/guano samples

Project Description/ Objective:

Standard Analysis

Analysis Requested:
 Apical Scientific Sdn Bhd
Lot 7-1 to 7-4, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: ngs@apicalscientific.com

(Mobile)

S1)
mparison studies is needed.

No. of Samples: 3

Accession No.:

guano samples
 NGS BIOINFORMATICS ANALYSIS SERVICES: SAMPLE INFORMATION

Fields marked with * are mandatory

The Sample Name should start with an alphabet and have less than 8 characters.
It can ONLY contains:
a) Alphabets
b) Numbers
c) “.” (dot) symbol

SAMPLE INFORMATION

Tube No. Sample Name* Remarks

1 WBA

2 WBB

3 WBC

10

11

12

13

14

15

16

17
18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

37

38

39

40
 INFORMATION

rs.

GROUPING INFORMATION

Group Name

Healthy

Healthy

Healthy
 NGS BIOINFOMATICS ANALYSIS SERVICES: COMPARISON INFOR

(Only applicable for 16S rRNA/ITS/18S Amplicon Sequencing Projects)

Sequence Region: ITS1

Forward Primer: Reverse Primer:

Forward Overhang Reverse Overhang

Adapter: Adapter:

1. VENN DIAGRAM

Venn Diagram
Sample1/Group1 Sample2/Group2 Sample3/Group3
Number

Venn 1 WBA WBB WBC

Venn 2

Venn 3

Venn 4

Venn 5

2. STANDARD STATISTICS ANALYSIS

The following statistic analysis will be provided ONLY when there is more than 2 groups and a minimum of 3 s
if there are significant differences between groups.

Deseq2 (Note: Min. 3 samples per group)

Group A Group B

Test 1

Test 2

Test 3

Test 4

Test 5

LEfSe (Note: 2 or more groups required)

Group A Group B Group C

 Test 1 WBA WBB WBC

Test 2

Test 3

Test 4

Test 5

3. ADVANCE STATISTICS ANALYSIS

Additional charges will be applied. Leave this Blank if your project did not included Advance Analysis

SiamCat (Note: 2 or more groups required)

Group A Group B

Test 1

Test 2

Test 3

Test 4

Test 5

Correlation Analysis of Sample with Environmental Factors

(Note: Metadata must be provided in the Tab of "Sample Info". For example: pH, diet, etc)
Group A Group B Group C

Test 1

Test 2

Test 3

Test 4

Test 5
RVICES: COMPARISON INFORMATION

(internal use)

(internal use)

Sample4/Group4 Sample5/Group5 Sample6/Group6

groups and a minimum of 3 samples in each group. Results will display only

Anosim, PERMANOVA (Note: Min. 3 samples per group)

Group A Group B

Test 1

Test 2

Test 3

Test 4

Test 5

Group D Group E Group F

ed Advance Analysis

e: pH, diet, etc)

Group D Group E Group F