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    This document describes research into 3D bioprinting cardiac tissues using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. The researchers developed a collagen-based bioink that supports the direct 3D printing of cardiomyocytes. They printed ring-shaped cardiac tissues that remained stable over 30 days of cultivation. Within 3 days, the tissues began contracting, with contractions increasing in strength and synchrony over time. The hiPSC-cardiomyocytes displayed organized sarcomeres and responded to pharmacological stimuli. Additionally, multi-layered tissues were printed using two distinct bioinks. 3D bioprinting shows promise for generating engineered cardiac tissues with complex architectures and improved functionality.

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    This document describes research into 3D bioprinting cardiac tissues using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. The researchers developed a collagen-based bioink that supports the direct 3D printing of cardiomyocytes. They printed ring-shaped cardiac tissues that remained stable over 30 days of cultivation. Within 3 days, the tissues began contracting, with contractions increasing in strength and synchrony over time. The hiPSC-cardiomyocytes displayed organized sarcomeres and responded to pharmacological stimuli. Additionally, multi-layered tissues were printed using two distinct bioinks. 3D bioprinting shows promise for generating engineered cardiac tissues with complex architectures and improved functionality.

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    10 views1 page

    2 2D Printing

    Uploaded by

    Sofia Botti
    This document describes research into 3D bioprinting cardiac tissues using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. The researchers developed a collagen-based bioink that supports the direct 3D printing of cardiomyocytes. They printed ring-shaped cardiac tissues that remained stable over 30 days of cultivation. Within 3 days, the tissues began contracting, with contractions increasing in strength and synchrony over time. The hiPSC-cardiomyocytes displayed organized sarcomeres and responded to pharmacological stimuli. Additionally, multi-layered tissues were printed using two distinct bioinks. 3D bioprinting shows promise for generating engineered cardiac tissues with complex architectures and improved functionality.

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    Downloaded from https://academic.oup.com/eurheartj/article/42/Supplement_1/ehab724.

    3236/6394870 by Istituto di Matematica Applicata e Tecnologie Informatiche "Enrico Magenes" del CNR user on 28 March 2023
    3236 Basic Science – Cardiac Biology and Physiology, Biomaterials, Tissue Engineering

    Direct 3D printing of hiPSC-cardiomyocytes in collagen-based bioinks


    T.U. Esser, F.B. Engel
    Friedrich Alexander University, Department of Nephropathology, Erlangen, Germany
    Funding Acknowledgement: Type of funding sources: Public grant(s) – National budget only. Main funding source(s): Deutsche
    Forschungsgemeinschaft (DFG)

    Background: Cardiac tissue engineering is an effective strategy to gen- bath. Cardiomyocytes were differentiated in 2D monolayer and expanded
    erate tissues for drug testing and disease modelling as well as for cardiac by CHIR99021-treatment and regular passaging. Cells were encapsulated
    repair. Tissues produced by casting show good functionality and advanced in a rat collagen-I based bioink and printed into support bath prior to gela-
    maturation, but do not replicate the native tissue architecture and hierar- tion. After bioink gelation at 37°C, support bath was removed, and con-
    chy. Additive manufacturing technologies, such as 3D bioprinting, enable structs cultivated free-floating for up to 30 days.
    the generation of hierarchically structured tissues with complex geome- Results: We printed ring-shaped cardiac tissues measuring 5 x 5 x 1 mm,
    tries. This technology has been used previously to generate models of the which remained stable over the course of cultivation. First contractions
    heart. However, these approaches either showed limited tissue function- were observed after three days, which increased in magnitude and syn-
    ality or required a two-step procedure using a structural and a cell-laden chronized across the tissue with prolonged culture. HiPSC-cardiomyocytes
    bioink. displayed striated sarcomeres and were responsive to pharmacological
    Purpose: Here, we aimed to develop a collagen-based bioink, which en- stimulation. In addition, using two distinct bioinks, multi-layered constructs
    ables direct 3D-bioprinting of hiPSC-derived cardiomyocytes and supports were generated.
    the formation of functional cardiac tissue. Conclusion: 3D-bioprinting is a promising tool to generate engineered car-
    Methods: To generate cardiac tissues, a commercial pneumatic extrusion diac tissues with complex geometries and improved functionality through
    bioprinter with custom modifications to enable passive cooling of the bioink designed hierarchy. Our collagen-based bioink and associated printing
    was used. Gelatin/gum arabic microparticles were obtained through com- strategy enables the fabrication of Collagen-based contractile cardiac tis-
    plex coacervation, compacted by centrifugation and utilized as support sues in a direct manner.

    Contractions of printed cardiac tissue

    ESC Congress 2021 – The Digital Experience


    27– 30 August 2021

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