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  • Improvement of Soil Cohesion Using Microbial Acitvity

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    Rida Boualil
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    This document summarizes research on improving soil cohesion through microbial activity. The researchers grew Bacillus subtilis bacteria that produce the urease enzyme under urine-based growth medium. They then injected the bacteria and a solution of urea and calcium chloride into sand samples over multiple days. They found that bacterial urease activity, which facilitates the precipitation of calcite from urea and calcium, peaked on the second day of incubation. On this same day, soil cohesion reached its highest value, while the friction angle was at its lowest. The researchers were able to improve soil properties through microbial induced calcite precipitation.

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    This document summarizes research on improving soil cohesion through microbial activity. The researchers grew Bacillus subtilis bacteria that produce the urease enzyme under urine-based growth medium. They then injected the bacteria and a solution of urea and calcium chloride into sand samples over multiple days. They found that bacterial urease activity, which facilitates the precipitation of calcite from urea and calcium, peaked on the second day of incubation. On this same day, soil cohesion reached its highest value, while the friction angle was at its lowest. The researchers were able to improve soil properties through microbial induced calcite precipitation.

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    © All Rights Reserved
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    12 views1 page

    Improvement of Soil Cohesion Using Microbial Acitvity

    Uploaded by

    Rida Boualil
    This document summarizes research on improving soil cohesion through microbial activity. The researchers grew Bacillus subtilis bacteria that produce the urease enzyme under urine-based growth medium. They then injected the bacteria and a solution of urea and calcium chloride into sand samples over multiple days. They found that bacterial urease activity, which facilitates the precipitation of calcite from urea and calcium, peaked on the second day of incubation. On this same day, soil cohesion reached its highest value, while the friction angle was at its lowest. The researchers were able to improve soil properties through microbial induced calcite precipitation.

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    Geotechnical Engineering Journal of the SEAGS & AGSSEA Vol. 51 No.

    4 December 2020 ISSN 0046-5828

    Improvement of Soil Cohesion Using Microbial Acitvity


    Yustian Heri Suprapto1, Budi Susilo Soepandji1, Wiwik Rahayu1, and Puspita Lisdiyanti2
    1
    Department of Civil Engineering, Faculty of Engineering Universitas Indonesia, Indonesia
    2
    Central Research of Biotechnology of Indonesian Institute of Sciences, Indonesia
    E-mail: yustian.heri11@ui.ac.id

    ABSTRACT: Bio-cementation process or commonly known as MICP (Microbial Induced Calcite Precipitation) on soil is strongly influenced
    by urease enzyme activity. High of urease activity the precipitation of urea and calcium chloride into calcite particles will also increase. The
    urease enzyme is obtained from B.subtilis bacteria with isolate number P3BG41 and P3BG43. The bacteria was grown under B4 urine medium
    at 37oC and pH +7 for five days observation. The B.subtilis was then daily measured its optical density and urease activity. The bacteria and
    combination of urea (CO(NH2)2) and calcium chloride (CaCl2) were daily injected into the sand to obtain the optimum results from the calcite
    precipitation. The highest value of urease enzyme activity occurs on the second day incubation. While the optical density was reduced on the
    second day, the soil cohesion reaches the highest value at that day. However, the friction angle value on the second day has the lowest point
    compared to the other day.

    KEYWORDS: B.subtilis, Calcium chloride, Cohesion, Friction angle, Urea.

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