01 02 03

USING ORGANS: TISSUE CULTURE SUB-CELLULER

HEART, TRACHEA, & CELL CULTURE: FRACTION:
UTERUS, HEPATOCYTES, NUCLEUS,
INTESTINE, HEPAR ERYTHROCYTES, MITOCHONDRIA,
CANCER CELLS MICROSOMES,
CYTOSOL
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 Tube 1 Tube 2 Tube 3 Tube 0 (0 min)
Enzim + + + +
Substrate + + + +
NADP + + + +
G-6-PO4 + + + +
G-6-PO4 DHD + + + +
MgCl2 + + + +
Buffer Ad 1 ml Ad 1 ml Ad 1 ml Ad 1 ml

Semua dicampur dan dipre-inkubasi pd 37C selama 5 menit kecuali

untuk NADP
 Tube 1 Tube 2 Tube 3
Substrat + + +
Enzim 1 2 3
NADP + + + Tambahkan akhir
G-6-PO4 + + +
G-6-PO4 DHG + + +
MgCl2 + + +
Buffer Ad 1 ml Ad 1 ml Ad 1ml
 Tube 1 Tube 2 Tube 3
Substrat 1 2 3
Enzim + + +
NADP + + + Tambahkan akhir
G-6-PO4 + + +
G-6-PO4 DHG + + +
MgCl2 + + +
Buffer Ad 1 ml Ad 1 ml Ad 1ml